	   SnapGene      &CGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCGCGCAATTAACCCTCACTAAAGGGAACAAAAGCTGGAGCTGCAAGCTTAATGTAGTCTTATGCAATACTCTTGTAGTCTTGCAACATGGTAACGATGAGTTAGCAACATGCCTTACAAGGAGAGAAAAAGCACCGTGCATGCCGATTGGTGGAAGTAAGGTGGTACGATCGTGCCTTATTAGGAAGGCAACAGACGGGTCTGACATGGATTGGACGAACCACTGAATTGCCGCATTGCAGAGATATTGTATTTAAGTGCCTAGCTCGATACATAAACGGGTCTCTCTGGTTAGACCAGATCTGAGCCTGGGAGCTCTCTGGCTAACTAGGGAACCCACTGCTTAAGCCTCAATAAAGCTTGCCTTGAGTGCTTCAAGTAGTGTGTGCCCGTCTGTTGTGTGACTCTGGTAACTAGAGATCCCTCAGACCCTTTTAGTCAGTGTGGAAAATCTCTAGCAGTGGCGCCCGAACAGGGACTTGAAAGCGAAAGGGAAACCAGAGGAGCTCTCTCGACGCAGGACTCGGCTTGCTGAAGCGCGCACGGCAAGAGGCGAGGGGCGGCGACTGGTGAGTACGCCAAAAATTTTGACTAGCGGAGGCTAGAAGGAGAGAGATGGGTGCGAGAGCGTCAGTATTAAGCGGGGGAGAATTAGATCGCGATGGGAAAAAATTCGGTTAAGGCCAGGGGGAAAGAAAAAATATAAATTAAAACATATAGTATGGGCAAGCAGGGAGCTAGAACGATTCGCAGTTAATCCTGGCCTGTTAGAAACATCAGAAGGCTGTAGACAAATACTGGGACAGCTACAACCATCCCTTCAGACAGGATCAGAAGAACTTAGATCATTATATAATACAGTAGCAACCCTCTATTGTGTGCATCAAAGGATAGAGATAAAAGACACCAAGGAAGCTTTAGACAAGATAGAGGAAGAGCAAAACAAAAGTAAGACCACCGCACAGCAAGCGGCCGCTGATCTTCAGACCTGGAGGAGGAGATATGAGGGACAATTGGAGAAGTGAATTATATAAATATAAAGTAGTAAAAATTGAACCATTAGGAGTAGCACCCACCAAGGCAAAGAGAAGAGTGGTGCAGAGAGAAAAAAGAGCAGTGGGAATAGGAGCTTTGTTCCTTGGGTTCTTGGGAGCAGCAGGAAGCACTATGGGCGCAGCGTCAATGACGCTGACGGTACAGGCCAGACAATTATTGTCTGGTATAGTGCAGCAGCAGAACAATTTGCTGAGGGCTATTGAGGCGCAACAGCATCTGTTGCAACTCACAGTCTGGGGCATCAAGCAGCTCCAGGCAAGAATCCTGGCTGTGGAAAGATACCTAAAGGATCAACAGCTCCTGGGGATTTGGGGTTGCTCTGGAAAACTCATTTGCACCACTGCTGTGCCTTGGAATGCTAGTTGGAGTAATAAATCTCTGGAACAGATTTGGAATCACACGACCTGGATGGAGTGGGACAGAGAAATTAACAATTACACAAGCTTAATACACTCCTTAATTGAAGAATCGCAAAACCAGCAAGAAAAGAATGAACAAGAATTATTGGAATTAGATAAATGGGCAAGTTTGTGGAATTGGTTTAACATAACAAATTGGCTGTGGTATATAAAATTATTCATAATGATAGTAGGAGGCTTGGTAGGTTTAAGAATAGTTTTTGCTGTACTTTCTATAGTGAATAGAGTTAGGCAGGGATATTCACCATTATCGTTTCAGACCCACCTCCCAACCCCGAGGGGACCCGACAGGCCCGAAGGAATAGAAGAAGAAGGTGGAGAGAGAGACAGAGACAGATCCATTCGATTAGTGAACGGATCTCGACGGTATCGGTTAACTTTTAAAAGAAAAGGGGGGATTGGGGGGTACAGTGCAGGGGAAAGAATAGTAGACATAATAGCAACAGACATACAAACTAAAGAATTACAAAAACAAATTACAAAATTCAAAATTTTATCGATAAGCTTGGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCAGATCGCCTGGAGACGCCATCCACGCTGTTTTGACCTCCATAGAAGACACCGACTCTAGAgccaccatgtctgaatatattcgggtaaccgaagatgagaacgatgagcccattgaaataccatcggaagacgatgggacggtgctgctctccacggttacagcccagtttccaggggcgtgtgggcttcgctacaggaatccagtgtctcagtgtatgagaggtgtccggctggtagaaggaattctgcatgccccagatgctggctggggaaatctggtgtatgttgtcaactatccaaaagataacaaaagaaaaatggatgagacagatgcttcatcagcagtgaaagtgaaaagagcagtccagaaaacatccgatttaatagtgttgggtctcccatggaaaacaaccgaacaggacctgaaagagtattttagtacctttggagaagttcttatggtgcaggtcaagaaagatcttaagactggtcattcaaaggggtttggctttgttcgttttacggaatatgaaacacaagtgaaagtaatgtcacagcgacatatgatagatggacgatggtgtgactgcaaacttcctaattctaagcaaagccaagatgagcctttgagaagcagaaaagtgtttgtggggcgctgtacagaggacatgactgaggatgagctgcgggagttcttctctcagtacggggatgtgatggatgtcttcatccccaagccattcagggcctttgcctttgttacatttgcagatgatcagattgcgcagtctctttgtggagaggacttgatcattaaaggaatcagcgttcatatatccaatgccgaacctaagcacaatagcaatagacagttagaaagaagtggaagatttggtggtaatccaggtggctttgggaatcagggtggatttggtaatagcagagggggtggagctggtttgggaaacaatcaaggtagtaatatgggtggtgggatgaactttggtgcgttcagcattaatccagccatgatggctgccgcccaggcagcactacagagcagttggggtatgatgggcatgttagccagccagcagaaccagtcaggcccatcgggtaataaccaaaaccaaggcaacatgcagagggagccaaaccaggccttcggttctggaaataactcttatagtggctctaattctggtgcagcaattggttggggatcagcatccaatgcagggtcgggcagtggttttaatggaggctttggctcaagcatggattctaagtcttctggctggggaatgctcgagggcggtggaggcagtggtggaggcggtagcATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAACGGGATCCGGACTAGTTAACGCGTCGACCAGTGGTCGACCCTGTGGAATGTGTGTCAGTTAGGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCAGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCGGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAAAGCTTACCATGACCGAGTACAAGCCCACGGTGCGCCTCGCCACCCGCGACGACGTCCCCAGGGCCGTACGCACCCTCGCCGCCGCGTTCGCCGACTACCCCGCCACGCGCCACACCGTCGATCCGGACCGCCACATCGAGCGGGTCACCGAGCTGCAAGAACTCTTCCTCACGCGCGTCGGGCTCGACATCGGCAAGGTGTGGGTCGCGGACGACGGCGCCGCGGTGGCGGTCTGGACCACGCCGGAGAGCGTCGAAGCGGGGGCGGTGTTCGCCGAGATCGGCCCGCGCATGGCCGAGTTGAGCGGTTCCCGGCTGGCCGCGCAGCAACAGATGGAAGGCCTCCTGGCGCCGCACCGGCCCAAGGAGCCCGCGTGGTTCCTGGCCACCGTCGGCGTCTCGCCCGACCACCAGGGCAAGGGTCTGGGCAGCGCCGTCGTGCTCCCCGGAGTGGAGGCGGCCGAGCGCGCCGGGGTGCCCGCCTTCCTGGAGACCTCCGCGCCCCGCAACCTCCCCTTCTACGAGCGGCTCGGCTTCACCGTCACCGCCGACGTCGAGGTGCCCGAAGGACCGCGCACCTGGTGCATGACCCGCAAGCCCGGTGCCTGACGCCCGCCCCACGACCCGCAGCGCCCGACCGAAAGGAGCGCACGACCCCATGCATCGCCAGTCGACAATCAACCTCTGGATTACAAAATTTGTGAAAGATTGACTGGTATTCTTAACTATGTTGCTCCTTTTACGCTATGTGGATACGCTGCTTTAATGCCTTTGTATCATGCTATTGCTTCCCGTATGGCTTTCATTTTCTCCTCCTTGTATAAATCCTGGTTGCTGTCTCTTTATGAGGAGTTGTGGCCCGTTGTCAGGCAACGTGGCGTGGTGTGCACTGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCATTGCCACCACCTGTCAGCTCCTTTCCGGGACTTTCGCTTTCCCCCTCCCTATTGCCACGGCGGAACTCATCGCCGCCTGCCTTGCCCGCTGCTGGACAGGGGCTCGGCTGTTGGGCACTGACAATTCCGTGGTGTTGTCGGGGAAGCTGACGTCCTTTCCATGGCTGCTCGCCTGTGTTGCCACCTGGATTCTGCGCGGGACGTCCTTCTGCTACGTCCCTTCGGCCCTCAATCCAGCGGACCTTCCTTCCCGCGGCCTGCTGCCGGCTCTGCGGCCTCTTCCGCGTCTTCGCCTTCGCCCTCAGACGAGTCGGATCTCCCTTTGGGCCGCCTCCCCGCCTGGAATTCGAGCTCGGTACCTTTAAGACCAATGACTTACAAGGCAGCTGTAGATCTTAGCCACTTTTTAAAAGAAAAGGGGGGACTGGAAGGGCTAATTCACTCCCAACGAAGACAAGATCTGCTTTTTGCTTGTACTGGGTCTCTCTGGTTAGACCAGATCTGAGCCTGGGAGCTCTCTGGCTAACTAGGGAACCCACTGCTTAAGCCTCAATAAAGCTTGCCTTGAGTGCTTCAAGTAGTGTGTGCCCGTCTGTTGTGTGACTCTGGTAACTAGAGATCCCTCAGACCCTTTTAGTCAGTGTGGAAAATCTCTAGCAGTAGTAGTTCATGTCATCTTATTATTCAGTATTTATAACTTGCAAAGAAATGAATATCAGAGAGTGAGAGGAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGGCTCTAGCTATCCCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCGGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGGACGTACCCAATTCGCCCTATAGTGAGTCGTATTACGCGCGCTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGCTTACAATTTAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAG  R   5   7   !   &   =   :   &   %   4   !   $      )   0   0   /   1   ,   #   ?   4   4   "   &   !         (   +   "       .   :   (   #   $         "            !   !               *         1   C   :   !   !   ?      -   &   +   *   4   C   ,   .      .   0   :   ,   $   0   )   $      3   >   4      ,   <   "   1   :   H   ,       &   +   .      !   .   .   #   1   )          !   '         $   )   (          !      #   #             (   6                 $      $   2      '   4   E   7   4   2   #      ,   +               ,   /   )         (         #   #   (   !      *         (         -          *      ,   "   "   4   /   N   "      &   $   -             %   '   )         +      #   #             )         "   0             &               $   %      X   *   5   +   9   6   /   '   2   (          (             .   L   *      &                      <   /          )   !      )      E   1   %   &   /   /      4       2   3   .   .        5 stŸ ü?GÊ;<=DEFS
sÑ¹O‰vwïˆÙ#¢TU'çOxŽ��WX”Ò Ó ì!"P$Q$R$ö$÷$G%H%I%7 ';ËÜuˆ¡ÍßOm@°žË>T¯	´	
@
\
ž
LÒuŠX‡¦@‰Ú±Öy²‘êþò=»(•Ô ï "±$ë$ø$! ¶ZeHG3	Mº`xŸð œ$Þó(ËÝe—y…Ò!$S$Ó$%& [ Ä‚Òý
Š
t|P®ù¡ægm`�£�VíùèP]1›Yª í!G$J%= i(çEPvÎÏÒÕèVn}«`ð°7	A
Ÿ
Î
„vã|A±íù!�®ž/;E½8Ó ëÝéÓ¬ · ö z!Ã!"k$²$Þ$ì$N%:  T ¶ Ô 	‰¨<®Ý‰¢”Pw!Rq�˜%ÞUB‰µÓXÑø:YˆZÛ`Ÿ*zB—ÿqÅ>û–¢ ð Ø$ù$W%&& Â Á,i0A§Ÿ?


j‹”d§ŠÅ²×þ>§%)”W Õ v!€!+"¥"#]#5$µ%% ÆÌøà±Ì	µ	]
Mh^è=6“ÂÝÍ!;‡J³Û’ó¼é¯ �! $¼$4 †‘)[7í4	è	›
Q£Û’	COay £²xêö%(Tlí[m0Z)}”¨ÁoÞã˜‡[zÉ!J"¬"N#! J”‰š³Ch!HÈB	‰	Ž
®Fj”ñ!ÿOL¡îÛ!ö!`"{#Á$È$%$ H ”fI+l'ð	
`»‚T„¨‚È�ãÕß$æfž¾A†3 ¯!Q#$T$%u% ·	¢
N˜®�4•õß6ôÌ Ó!C$Ô$) ¿"ƒÜ
‹
’
uQçüAbhnÎa<(3W«Ù¼'Hy´ÌÚéK!}!$;$¹$K%0 ? \ ˆ ¬Å(þšR	™	Ã	ö	M
Ûú3¢ð}ž=‚—¤å½0xîú´é^j{Š4 [ /!d!""Ï$0 ‘ ¦ 2þHÿÓ=cØ}ªÍÀRÏl´ºÕ$lç2D‰^cmQV�öœõI•à>Z§!«!‚#ù#H$/ * šê» ÖÏ�	¯MM±ÒB
>Žju�£¶Ï¨Ìëþ2²¸Áì#‰«9“ ž!î!¸#j%|%‡%1 åF’ÐÓÝÐÚaÈ 
¡\hæt>²äîúekR^‘	-0<{žÃ°¶ÛÞê‡­ {!é$&, jlâ‘QSU`wnÖé.=?¶ñóõ÷ýÏ
%Ÿ	…:ä„Fd9T»Á×­h D"#ƒ$š$³$ß$# v S`,3~¬”±8	B
rw¯Ÿ_y¡ #½Ô
�¯7 „ "Ú"Ë%ø%?  b ¾ !)èbÏ˜HWo°
ÿ
.+2n}çB
8"ybtÆÚæH~¾ZÔIäìÈLÐ
 ¸ ÷ !Ä!Ì!ê"d#Œ#l$“$á$í$,%O%Ï%ï% &4  Š©Mü2€•ë0xßÛäçêï
C²‡<5;‹ñW~Þ†4|%O „{˜®Ó„n¼´  !¯"x#®$&4 U Õ n¯ÒØÞŠ£§óA"S¸ô6V7Ôþ &5YnžØãB1‰[aÏ‰r®?—úS^I 3"û"œ$µ$ú$X%" · ÒyðQr™âù&s�ßZ€Š¶ÒùZÜC³°·f* j ü & 
Ê=ÆÕ5ÄŽý½
'c«oáð/ï+ Æü£�¿ñ N!/"Æ#L$p$~$Ù$ç%! PW…¨ë	"+IÇÿ/kŒ‘•È¨Ìöqœ›hÍX  !w!ó!,"<% Ã äýjpŽØ&Y ¦
ôû?·åˆ �!º!F"¼"“#	$+$6$…$¶%Ô% . æ ‚ÂÙB“@ 
?Ð>eÆÿ¨l’*3QU"…"‰"3%­%( ™“-<Â1Æ
¢Ëî‹ù�³ØÈf&t¶ßV“q•¦¨ Ö è ="¦"²" ###^#+ ³Çò‘—õ	*	}	†	7
�
Ñ
FN>D7:”?Ã€ƒ"%u¯Ô�´ô½»Íþ!"($" D ¼(w‰á¥²Òùÿbqò
ô_)dÖT
ðKd±Ù` ¢ !9"½$  P ƒÍù%>	¶	
^
ø
ªdéûˆÞë­	a“F™9ê. r!!$'%. à œ©Ê¢¶ÍÜç¬	å
i…¾ÇéH!{§îù[Î<å;ˆ›½ËÜÜZÚ_õƒ° Ž!k"Ç"ò"t$ë%: ´ ‡’¬½5M®#Aî5	
œ
zMá8DPbz¡¤j¦ëô÷&óÿ^1Æ<¦º¿mo"áä¨çˆƒçÐ!O#÷#$³%( ` t ¼ �*î�é	»
$á�¼Umî¥+D¿Ù
7ó™\† !Ê!­"Ø"‘$:%Í%þ%# ² npª]_	•
x‘™¤ÜH
G\\[ÂËp›13Bmß©#$$ y�òbr\_8¹jNR“³y)ªÑÖHn*~•©Ä%5rœ{t K"Y$ &;kM]i_�
¯• (q.€ƒÖÇ¥'!"ø"«#±#Í# K�Š¥€É	C	Š	n
çGk˜"GÑM|b¢d Ü ÷!a"%#|#É$" « êjÀ´D"	:	a	Ñ	›Þtò7Ui‚œ9ËïùÜ!"Â$%½% •.›�IV	o	¦	Ç	ú	—
|W…±Ëa’PÆ÷9  ' ¯ |ëJzâJì…{)Ž£­äZr�?¤Ðv4 ì 8!‡!$ x U•áï–(ºa¼-ÿãÖàKçB‡"´#ð#/$£%! I ,mx°ñ	
D
H
¶
à
l¼ÊIƒ©ÍÌ%*¿¯D±Ã°!q"Ü"R#V#%! ^g³	ŠÆy·UUŽZ¡Ð¦]ƒÉ†~7gŸÜñÝâ" #U$v%—% k
ž5ëöÅLÖxíu"'4 $D$Õ$%0% ¦f‹ø-	€	×	:
Â
å™¯ì
P˜àl°ùiŒHC "g"î"C%ú% )>¸
x¯ø)
£
	“O˜ÙÎÇ7Üõ”
Í;EÔ!$ ¦0^k–e¥†„·Ä)Ö%* Y À  Cq¹î	
Œ
sèýÜ.4RX€\(°ÙéüÊØÛ 2Ÿì‘ ~!Ù!L#A$º$Í$L%h% Ö#^ƒòBciob=‹~‚µdL!$n$ö%& % d‰¸
“
vÿµZÏé¤ÿJÄÚ½Izñ!Î!ì"##�$.%¡%1  # d ñ ó À	„ÝRÔ$M‰p»ý)o‘¬þ "BŒÅÖ®§ÀÍÖìžê;!˜!!#Ž#<$ $ã$ñ%C 9 ] ßºõÊÞ:Jqt†»¾Á›PS	Ä	÷	­
-•?ÅÖqÜé;{™£ñÓôÛ,-1uyÿ“ûÂk}Ó­`©îq Š!ä!‰#�#>$Ð$á%ó%:  f k õ 0CY­ÆPšµ)ßîçðk„
%#4~~Ÿ>˜v;TƒœKë–ïI µ×©ì  *!0!=!e!#"3#c%! {Úaåÿš	½¼û�Átƒæ¾@DˆŒq—äêò	Ï5 N \ ["! @ ‰ t°{_N
ß–÷&¥d–±ŽØÆ_cŽ|‹}ÒÉ ç!Ð#[$å$? <  ¿”Íg>~ÙÝ
T«ÊÎTpÐÖ:‚£{«JM›ÈèèŠD‘ÑDGW~�ðn–vNXkv ù ¨!¬!·!A",#ú#ý#I$„%Ñ% Û 3IÑÔvÝ	ƒá7\¿�öPÊùâº E#f#ç#•$- ’   FWdÿƒYËd«~ÁSµØ3ERdGbn»Â	'Öá?t{ìÁ ]!/#ƒ#g$§$ï$& | § ù+ê	9M²
Wm»à%m_,ýR“÷KîJM[ÑJš!â#?%�%Ú%+ + ×¼ÓÞOÓ«®�ž¡ª+BavŽ® $r4ó3³¹$;0  Æ!Z#®#Þ%ä%* ì 2›¡	j	Ô
*¦ÇDNt
¿þw¶½ÒÐéï" {r2¬y ” !ï!¹# $¢$%_%4  `Ÿô#L[ëlÔ•	Ê
4°¶N?r¤0«O7;—ìÿ6>æ¼Âí[¬:<ã #!M"Õ#ÿ$Q%k%}%ˆ%Å%C 	 tÂ×ÐàîJ	s	‘	¡	y
0gÁÖ+¦¬²CPoª¶¿ËÕàgk·Àá,D€¤²¶Ç©@Í7XdmyƒŽŠ—Â²Æ ·Ÿ!û!b$, :ÊÛžÌÞÑ�É:R	r¸­_u?›åfl_€ŒìòN�šýñd–'°$Ò$ê$õ$F%. Ó h§æ÷O+h~“ÑÔUv/_Ýˆ´g]iç÷ 5�’
.¼†ÒŸÄÜÿ® ¶ Œ!¤"#Ý$& G �kì¡
¢—N“SìZ|†I"«"z#À$. ‡ � «G™þ'ŸÛb™Õ‘
Ú2L³ïû#/kw	1=C¢³i±·ßëˆ|!!"·#:$¸$0  ká�äRTam‘—-2<>Gµ“òôöü¯
þ
 *q[åsæ=GSº¼Â¯†®	 g c#‹#à$: mLx¦Ôòû14ê/@Ã·áóørãæé5Yî
&±ý4†�;A04Ýà.…3{$îˆ­ÒR¾) H i ú"Å#›$´$, ã’V~;o�×%’Å
!Æó¡þ.ÇÏ=øe›sµÞUgp¥ò!<"E"„"±"ÿ"#’#„$$ ²›'vˆ¨É¤µøþapÐ
ñ
E
©(Gz:Y°Ù^ºØq!8"j"ñ"'$0 _ ñam©¼í4L¸­ŒM^	º
�˜#GàF7i¥óÕò*$0l¾Ø	q§æ‚s … ¨#ö#$Ì%ý%) Œj-i¿L¤\^	9	U	n	¥	Æ	ù	s{æ„—°Ð6~'`-‚›Õa8 c $ w Tàyw•²C
k‰x,ëT�Y âÏz¢…}£u°Ü†!
"p"Û"³#.$ (=¥÷Ö	(
/ØÍjOd—¤ì!hÕB "$%ù%3  X c ¿ ð ÕBcp¸ˆ
ñþY#ˆÛIQý[ÄÕØ�ÉÕðž!Í!ë"K#�#@$m$Ž$Ÿ$Ì$â$-%ð%õ%&> Xs¹ÉO™´Ipsz½Àäïj¬
,$>"p»Þè
}�ÀóöÚ+cu,?St‡›•|ëñbÑ M )!æ!Z"ˆ#Ï#b%4 ; { Ú ø*VéÐ‚X8Êª±
o~9�¢zªÇQçü}JmíÕOÉuLM¹ ø \!e#f$”$î$Ð% "K /f)3¥ÆCÝ¾f¿£zå«!Å!ú!­#a$P%ã%, g�ªÚN˜ýg}&.Üë9³·ö’14Žë¡»ñ…ÑhŒ™&þµ £"#y#9$¯$·$<  ¥3uŒ–§,1F$àò÷qåè4X~ð
 Dòü)pœ ¨-3<Fß÷yíX‡¯´¬…fo¤½ f °"þ"b#Ä#&$" ‹ði£ì ]	m	'
"ˆßåE6ÌSŒXÏ5c}&,Ô`Û…!o"$1 W ï rJUÔoÈ�·yÉ©«
#(=¤¿€¡ò©Ù‡>†PeP¢|êÔKL !ù!Y"ž$Ë$: V qTÓÙ¤ft¢ÿ+E#-ö38W}ñû!(oŸ¶ä'Dõ2x=b…OÐ†‹«éý_eÚK „!¢"ý"#�$¶$H op°56‹Œ�¨©ª¶sœ¡BCDTéõJ27‡ˆ”›S
f
ðúÿ '6ZC+MN…Šs@‰Š‹EFGYZpûüdJ Ž"¡"Ë"ü"9#:#;#<#Y%Z%, ±7Ž«±	ù	…¹8K�•œËŒ	T
g
†
8Õ\†t¯åùRH[q˜»Åè½!4"Ì"™#   Ö ß·ô�Uêx‰E	7[ÙŠ,GI-AŒöT!?!�"5#=#û$[%& 6 q #ŽsúVÌþ0	ƒ	�	
=
h
‡
é–®ÝL^°Ü6±¸I™éÊ+ ý ,!+  2Q¬
GúR‚Ït�à[gû	9DÓ%mš$]´æî~ãr¼ÆÜñôn!5"š#. m ¸ É ÷ Ó8z�²äñ
¨ÈºÁÛ'9L�Ž–�·r€K“ïgD ú�ý\g�k j!œ" ²ä†šãU
‹ÖúI[ŽÁó‡ÓuWS¾!¿"Í"'#ê#! h MÖä	Y
(JÄâ�Óæ·«1axUÀ¥ !5!0"Õ"M$q$ü$\%e%è%. Æ × E[¯Ç¸õžÅá5�¾þ05mŠp
÷8\dÚ‹6š»0˜ ý Vm›‹ Í 2!g!6#Ú$. 6Œ6ðV+yÁF	s
è¬`@µñ°Åð#,ùO¤Ç.^sR�÷� — Þ @!G!O!%"c"�"l#~#Ç#$#  û Ë>àRëÂ¾
_'-HpWVžK÷ÙB¤ß~ ½ ò >#¹%1 H¿X†Â€©Í1	Ž	²	æ	
>
ˆ
JV� ’�Í÷¯ÃÐ“Àås�k�Ý“²¹œY Ð -!x!ô!)"U%) Þ ä QÛ�ûWÿ„	ª	#,�Öù0blÉ©ÊôïY™œ_±JÕËÎ, ^ ‚ þ -"Å"˜$ r ­ $	i
àê�–ý':Š7CšÉåÚ •%  7 ž .^}th	Ÿ	ì	¿È9—Þr9B�ŠýâMZ«êio !!=%! ½k�S¶ZuŠ‘á\h		
ì
Eü &ng¢ï¸äo!6"G",$%' Ä R¯Eq÷ýƒ3HTe§¿ü©
õ%^„¢Úç$Ýæò| ‰ ‚!»!½"
$7$·% å­»CÙõûF¡f	§
Ô÷›ËµsÇõy"{"  3þH'Ðt:@½ !}"”#›#Û#†$Õ%$ ¹ ƒÃÔÜçZå¬×Ü(:Aò'	z	4
~
4Ñ�_ìÐ>÷û´!á!]"†") 3 n „ ç ø ¤l{³@ò©»ÂMª‚�œ	Ó	à	ò¾L” ”Ñ“uñl D!k!V"¿%( Ê ì9ÉC[”ž<	!
@vÁ?fðùtžûž§m +4R]h‚«³7Š"–"�"4%  / �ÚP£—¸²©sÇÙh©E¡—‡ÍþÁ&# ®% š€ä­$£Ú	Ç
?v…9×gæ1X”T¯Œ© é ">"! ø.=c2›Z	c	þ	V
Ìïû\�ŒÍèŽ´ôW·ô3@r× Þ!#_# ³Ãç‡Ë	×£’+FŽ'u·àv�;–P ¿!"§"À"	#(#%# ”å×
Œì;ž	JÂ’/�úÙºÉˆÔWShgÍ§ô³"Î"#ë#Ä$¦%# ´˜Æ@	‡	
Z
™
O^Ï‡?K{Ì;•ã@Ôk&%y¼U "N$s%  B N—È× å)Gù8‚Ä�#X»Ú¬¦ !!!"1")$r$ç$é% ö	+	~	Ï	8
EÅ„v¸‚µÂõ25£ÎÚÁ6!Ö"  i ê ó0’	q	‚
Ò
Û
ç”¹û@°5BÕ¾êbyVÿ¥$ý$]%f%( E Ñ Ø °È)J²�âõ6�³Óú Knd†eïY*ä¼™L¡œF a :"_$Œ$¾$Û$6 Ç eŠŸâ¦Æ¿Çÿ6v…‹’q
ø]ÝŒQD§³¹Â×UÍcñef²ã÷ /þWnÚ£ Ë 3!h!7#  F\½ ¹ö1cr½X	¨	É	ü	9õ`¿7í›‘Õe¿ŠÎ Ù#“% — Ï xžÅÒó
Ûe1Àü Œ !"]$y$  Q ÏC&dG	«eÈàa”È :ø‘Ž H!s!é!R"d"ç"#�#€$ 7�?}zéáA¶%[ßì®$úPš  A!P!&"$ ™ Î7ƒ,Â 	N	·	¿	_
ê­ü‰ò
b~¥ÈSþŽ�Ô/ ˜ œ S!’!"‘"¿#(% € 5„úñWÔ
t
ù
a·±Æñ-¥G/_të ; ß W!u"m#È#"$$  v­Ý¿
õ
` ›q¨\ÂÏã³Óio‰Ì�ö± ¾ ó B#Ò#$2${$º%Â%×%ì%2 á Ìázª#aì§ã	P
<(.I˜¿ê("(XïWŸV=æéò[Û?  !!�!l"È"?#H#i#–#¼#ó# Ë)£»­	æ
j~wÈ^<L�øõ£ÝR~Ý#u$ˆ$!%' ‹ ü ?�S·ÃÎè/
†¶ÂäIg3f|úz˜±œ¾ÝÚ`ùCN„¥àNó"4 &Y‡ÃNdl�û>F¯C®2	³	
?
‰
�
K{ÐLNW†ž¥îø‡Ø¡øæ\0Êé<º©x„Ñ î Ñ!ë!E S µ ˆÀÅ­Îç Qp|Œ—¦ª
$ï6		
Í
Wƒ“â9c{Y°Äìø<­�Á _::ž7ý=I¦A–pèÒèú“u!y!Â!*"$j$V%´%7 I…“ˆ(6²*Bgç	­Ú‘­BEi�¢±wƒ§õ'3k”ôÇ“ŒÀânÔKÞ#âå�@Z2 ®!õ!_"P#%4 ) > 1¾é<Î×Q	�	˜	Â	õ	L
Ì¿QÎk¹Ô°Ñæ2tŒªÎl§»ŽôH”"Gx³‰èZ .!J!ª!�#ø#${%†%2 a u ½ R _+ÜÏÙ$-ž19gm	as,}�ã"ÀÇÚKÖ	¬Ï6 ƒ Ë!C"Ù"é"#‚$’$™$+%Ê%Î%ÿ%# ÑÑïÞüŽ¼
6%m×âªŠðVn}ï²¯ù]mû ÿ ."®"K$ å ÁXê	ú�ÊíËõ‘š¶PäÌ‡ ç ¹!T"ˆ"»";%¬%Ó%, ß »‚=�–Ûô 	)	|	…	«	
6
€
ä
c„½Æ¦êZš®Óc€`E˜8ôÌý- _ ¡ !Æ"&%+ s ± ³ » oq~€«\^i"@yLŽàþ];¥ÃÊš24ACn!à›æ9%²% %:é€`	–
šÝVÊT�pÅ8ÆÊÛ &!ª#Ì# ® {î]ê	G
ß
áIÅH»þ\(ÌYqœ>Ëð®CU#Ÿ#ï#–% ®j
’¥äë—]	“¯øt‹(Df"$% ]í	rÓ´o­û¦�1� :!—! # %, 8 /BzÞ`9ºí^O¼”Ä:z˜•Ò:C‚‹þJê–ãÅ¨Ò¬_|p È ‰!ã!2#Z$/ Ÿ EcÌLfu}SVÉß×ILš+F[a�ÐF’¾ïºÁ&sëIjáu ¶!+#.#æ#ü#>%ƒ%Ž%) _~žÖóZskI	i	 	ÀµÒ*s¥«O+ªNî!:?Ÿò6/;x "!L"Y#ÿ# † ¦9T^j˜	�
Büðc•…H"ª"¶# �K}Óñ:l‡�’â`í
ý
°ý'/rØR¹p!7"ƒ"ù"( '<l¤¾·x[v‹]	Õ	¹
Fz–háN–£)/g�„×p¦å	"§#²#-$% z W÷¸ANi
!oy{ðN€È(![!¬#Î# ŽïIn—¦p¨ª
§"ËWÓ%£e ø!X"$8$Ê$# S°µrøþ,„I1|ÊD	‹	ôH…„èQDºçcÙƒ!¼!�" "# 5 p "ãF�ÎÚUfýCèHl™À#q­îKŸÛœV}âóm!›"¾"&#é#( Å ú L5‹½/4*À	o
ö&_Òå¯Ä"øN£]rU`lwÞ} Š ¼ Ý F!b"Ô"}#¸%! � ¬ Ý -ÁÚ	g	ž	±	œß�Õøœó8€ü&9j›ªÈän ("—$T% ®¼BDôöüµEG‰¦
¨
fÊƒ¡Ù#z"|"%* £æëkY«ÖO¢;	y	Ò	ß	uø^“–�Ï¦Ì=tª²ö%ðú" C!³!¾% ¬#¢b	ëÌV¶:?RÌóÝ!""Ã$%¥%  é –/Å?	p	Î	˜
Î†zË“W4AÁéxT r%( – dÿIœÄÑJu„‘¼W	§	È	û	c…X¾ãC²¸Ìbeâ.û~¾‰ "Ø#x$‹$ 4‚¾	Ç: › R!V!t"~"œ# (â	;}ã']U°QøM!G#h#•#Ü#ò#$1$‡$Á%Ö%- ( „ÄæckEo{–	);fB­P	Á	ô	K
K‚¾AVh…°í‚†¸9å[/çÑ?w1 í ^"$i$  ^Ð<ØÝó(	{	
5

ã
58ì|!�Ëø«ü5 æ ‡"º"#*%É% ° º 9è}[\­!F
ãÄKºß
S€¤®sÄ@Ê 'ï­T#8%* AÕÝYËìK]|´ÃÒI*`�Ïí >žÀ§Ñ^¥ .0:HÇ 9!ˆ!µ!â!1#X#‚%�% y … V¥£|ð9†—Ô	¸
y. M•x×fbo”„‚"µ#, 4 o ù !îHm–´â¼ÍÙ.o)0B¦ó¿¬ä!JVƒUÒáCT_kvØE!l!W"Œ"š"Ÿ"Ó"" œ ,¢AóªN«¡�	°	Že÷ûš’• ÎØ>¥©"±$òm ²!"
%¤%" • è cÃƒ�»Ä>	á	bÍ½T3dá-Lèw}ˆS š U!s"ñ#$0$q%À%4 ;[ÊD\• {ó	
E
J
â
�½Ã7J³¹ÂÞ@Ug„¯·8H	RÎ.¿&æÊ-¬/9GÆ ¹"S#W#7%�%Œ%È%/ ” › í¢…-n/ =	·
AxÌ«öú2I™U‚eŸÍ¤¨,anvà!S^jƒ°×±!r"�"‹"™"ž"	%p%N ¡:yz.Ÿò	I
á
wËªñòóôõ567G1u€�½¾dŸ ¡¢£ãäå+ÀÁ,i�´µ,-.8EF²ÄÖÃ Ä Å —"˜"·"¸"Ý"####q#r#5%6%n%o%€%‹%" J Ë …±"	¹	"
a
mpvˆün,AÂ5�¶³ÅÞ"#s#%›% ‘Û	€ÇQ[¢§^ÉFÿo8Ã6‘´ß"V$%& 0 Ì f$ÆQ¤²˜³›ª¸V�Ú1‹ôfq¢)‡˜ý Âî“"…#Á#$ h†¹‹z}VtÈÑþ.ÚTwª»ˆ -Ý·Þ$ ž ”!##¡#¤#˜%¯%- K ¢ _´		#	º	#
b
nÑ õ„Ê3di‰àäÎ›#(Bhò'?Æ_!£!ã"t#©$ñ$w%œ%  ü’%@RŸ:»h×aTvÊ#M¿U7’ ª œ!E$Ö$% ûð
¤â6\w†ì÷ÆØMyîG��1% © ›®l
´
Èº£(5ê "à"$W$A%% �ÜåÛ	È
�uœ¨_ßç2• Yp•09°Äµ á ×!?"à#%Ü%' ™§gù%/Ç³Ã
–‚«Íü]¹�WÛQ™±ú\j™¸s' "h"ï"†#D%û%) OgdŒ3™ù.	[	�	ÿ	;
W
æš´�½2ŒéõI‘õÑ*4IÃï™#`#Â#$$ 1 ‚ >YRd	Ø	°œð�ÎXr/ˆ�1AÓØ ß!w"”"Ã" ~ Í  óœ¥
í�µáùgm£õþ¡L= D P"‘%+ ¹/y†°èùOŠ
ºØWuÒ“8öû«¼ù¸Òáò<IFÅßŸ •!À!#)#Ê#ä#$%°% ¶W‡Ì	¤
û
 oÉÈUyLO¸Y!"¨"¥## *7?ÄiŒ¤{™ÜcÏÿ,/ó—v�<!ÞíQ 
##o#¢#$%™%#  -´ìîˆ*
Y”P~€Ú¹G�ÛÝx•(ÎÐðw‰.—% Õ!Á" £ µv
§<_ûlÚ³ÈÒÝÊ?jav€‹iÎ¤!u#Å$ $	$
,
c
Ø
é
[o�í1
KÃ“!ŠÕ*Ï×óÇä"ò$  æ`;Ö»	0ž—ö§Ë4.z}‰ÿ…¸á)@¨´"#ì#%x%�%)  L • ¾
	RŸ'fo¦»‡…
OeÅå*9§XTihœ$C](õ`!ÿ!Ï"ª$§% Z µr
[
tˆ "°ÕÝS&w±ÜV“ "$F$O$%   Á DAÐÜÌ D)Ú$¡V « õ !\#4$»$×$M%" “™ &ÇA	ˆ	ï	
�
š
P_Séþl/5Y�§N½ í È!Ú!M#B$Ç$t%0 ¥ ý!º©@La|�Í<–ä;A¼'iˆµØ]b&UËêõýÀËÙ=z83’ c!�!¦!Î$i%  _Çã7djQ]xƒÅ^ÙåcxYzHµ€!è$î%÷%& ÉÅ×ÚbNƒÎ­ƒ¶eŽ»³ M!2"w#o$}$­$æ% - O�˜ü„Ø¥*H>pŒÇ’k2§ !$*$2%& C O ñ$¡Ñæ÷
óCèú9c‡Õ >Síø‚¬	$ät¼ÊÛï‚!"s$ê% xœ
”
w[©»ÐG¤Å¹¾l6ò!Ï!×"$X$�$ ª 	Ð	m
F‘hö{¤ø""÷"$#°#¼% & ¯¹µ
É ¶¥¬)J6ÏÛÂë 7!á"¢% weŠ÷,		9
Á
Æê…Ä KÕÛkwƒ¶Ã	:&3Gí"/%B%$ " $ ò 
‚·LŠè}£ºüþ-n�Ã!A‹¯è¿×ëcŸëØ!"#g%%  e j ô ÙôÝ¯…Þæƒ
Õ%s•p’°H�ŸÁ×Ö�¨Îí <!�#=$ä$à%ò% ¾“Ü	Ü
‚Sà6CCøzÐWÀ ™!@"D#á#¦$Ù%X   ë Á1Óßí	r	”	x
É
Ó
Õ	Nýnª­©µ¾ÊÔßqv� ©µ¼Ñ*A`­àÿ#/Cq±µÆè36–5=Wclx‚�»Zq–Á1:±Å¶   â Ô#¡$þ$%^%Ä%Ý%* F � Ò Éö�Ë*užœÔQq‡f–¬M^ÙKš".Yjv‹ë²M‹! "¿$Ñ$Ü$ô$E%5 ±àšãú&0È;£Â‘´´ûo®
Ä
Å3@ZÆÎrå<Ü-2z #šÝ]¹®ÑQ( G ñ!;"i"ð"Š#‘#+ ^ ¨»hßKKöLT	¤	Å	ø	j�—.ƒ×+ê¤òÔiñÎëk~š|¢tA b r õ#$ü%9 : Ù ³r‡¼¿ã7îeðýºÅÝ	|ŽXÜõ½*+Rsš”üÃû×lÔatªï�å!J#‡#?$`$e$�$a%â%ô%6 î fhÇ|‹¶%Èñ \	l	&
›Þ,£‘0¾R 3E�¨ØêöÎdìºð¡g³+J n"a#Ã#%$/  4e› *è7w†Œ“šR
e
…
ïùµ&*Fw<„Lªäø,ˆõXoÄ
!>!Ê"#4#8#˜#'  l È ö 1Pã�§ÇÀú	/	‚	 
<
$�•J’òfÒ]í5ü€ÛðÉ+!i!2 g DZ®Qàï4ÁlãX
r
ç^5?™´ºÃV€U�—Jö¶Øª0QŸÿš – ¤ Ì 1!4!f!$"k#d%( ã G]|¾©	å	¾UaÉü8ÂîÏ’¿A‰äXr˜áYŒ‰B’Ô] � Ï Ù Ä"”% º2Sds¾	e	ë
ö¡ { !x"Ú#  2 ƒ Û?Z©ñ&	›	3
}
3±¨Àñ½Øsë
†ÐÀ6à!\"•"( ÷bæ Y	Ê	Ù	ý	Ö
>:�‘.‘*u„œç8E�¹Öå0Žó2gf®‹O  A Ÿ
Ú
]øj¸ú?ºÙ$4¢Ù~!"¤$æ$ Î Ð ±ô�ÆÜîPì¦Á �¿ÔöÿdE Ê \$^$’%   ˜ ÎB|cÓM	à$Z¶Çß`}¤ýŒÓ � ‘!è!Q"æ"¾#. Š uy¬"`¦º.
O
ô
v—šµÁ'f2ey—Áâ²Òèhn�ñô¢�}M> !A#»#Ñ#z$ %L = R %0‡'èÍ±ú=P‹¥Ö—	Ì
¬ËÏÙ¬€XvÓ×2;¯j ¬œ9‹©Í÷6’ü‹áH¥@•ÉÓèóJG!;FwùY’I!t!©!­!Á!ê!€#%z%…%* ºÐÀ•ÎÚlfƒ‰Å|`ré­Òbâ—Æ®µOãl  ú ¸!B"S"è"J$�$%%«%Ò% zíéh?Þ
UÉý’É÷X™o=ÅB%!e"ž#Ë#î#%±%& DeH	U»qÑ”¤KN™éE9E‘¬½îñú¹àw � –!#*#-#å#û#þ#Ÿ% ö8Ž·@JÒwü
àAûQzMïZ!©"¦# 4+{!pÊ®Ãíž÷\q›P¹» è# Ü X~Õˆx	Þ	¥
êÉ%8]QË<©ã! B!'"–$S% ½	Í	„âyÊâB·&\¯VÀúQ!"F#g#×#w$Š$  8@X]Ï bjne¬O	À	ë=Ã ¦É]ÿ�îª0 å #0#h$)%  +b¡G8ÌØÃ¯	�¥dS cw
‘×=T|#R ™ T!Ò"$< “ š  „Z„-!	¸	`
€Â6²¸ÝT®¶4F0HQo˜‡-T¼câm+@`uÉßŽ+ÕÂ €"¶"#p#%m%%Š%š%Ç%/ ¡ e#Å|šPÐÙý -0Šóô2SecºÈßã(š"'í>� ^!“!¢!’"#„#£#À#¨$ð$ ¨ úïû³
á¹ºt›Þ`S”L¾/€ à ›!Ö!ß#@%Û%  } � ÿ N˜òX
•�¼H�ø.Ð[0ÒK˜& < O"v"Â"#$�%)  6,.…µëíVN‰ú
XÛbn¸òú–øÏÑïñHxKÄNìX!n#É#ã##%!  
:Õ½+
u
è
ZQ&n²ÇÜ¦	>N-y|þ)8¦`uŠ\þ! ¤ ¨!`œ¯ÛËC‡´%¦ô< ô b!¥!Ç![#$3$Æ$) , N €ÄÆÐÙö
=açPÔR]«äbwãs‚ÉÍ´‘j1º² v#|$¬$å%í% wvÀ
¨F�ÃÔjg«k9%F÷"ö"¯#»%E  ! Ø®½¶ÒÞì“	w
ÔßKrüm©¬¨´½ÉÓÞ|Ÿ¢´Ð,m�¯Â.@GpŠž´À5Bç4<Vbkw�ŒºŒ÷Ï ¿ C#Ó#Ã%Ø%ß%1 Ž âœ¢í£	ipŽÄ2LÄØJ)hð!*XirÍ"ŠêêÖÜ{ÐsP@ ð!I#ô#$d$ó$`%%  í 3{$Àâ	k	%
Q
d
î+¢)/E”¹\©+ûPž‡ôÈ• 	!m"É"j#—#& â ¨ð	%	ä	2
|
Õ
ê
2=T§È-)7 ¸×ãWà‹f…‘­z € !/ ÍA«!b¹L	-
Ù
\Ou™´ÀëÀÿ#Yix·¾Ó>Ñç¹Þðó#¡|3Ø!�!å"@#º#½#£$%/ $çìÌ<¤Õ–	Ë
k1ˆ®ÄiŸ_q˜ü¨Ì5‘¬Šö8˜¤í?çE­Ä´âA$!í# %%y%ž%ª% õ*w	¼	xÉ­Â@[pî

;¨Ö#v$‰$R%4 a \Ÿ"am×®	5±·O1R/P~ <†¹ÇÞâ,Sb\l™&*_È"*=©ä ¡!µ"Ñ"#l%~%‰%Æ%   þ M—U¼ç
¸sš÷¥=Mù{Ï 7Z½Ãý!N"Þ#"%2   M uÃÅÏØÑë’	§æJEQ§¼{³l†Áâ-F��¥³¿ÈfªAæ;8Uj‹E¹öa! "õ"«$3 
 � @ ¸¿áï	K	¢	1
{
1hÃS¡×,³6g~ )hq·ÌÖâh‰¸¸ßÝ¨ez„�úO�†Oc$. ý –ž!Tv	‡­·Ãhpû¬Á™QÆÎ+YaZoDk˜Ã	%^³Ç	§á!) !ü!Ð"©%.  Œ Äéêt	u	0
z
Â å45D}(gp«Àáˆ²EP·ÞÛÜ:Yn�ijD…¦¸ô"¨%  5  qGACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,GCACCCANNNGTT,AACNNNTGGGTGC,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,CACCCA,TCGGTC,TGGGTG,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                      F                                                                                                                                                          	                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
   
                                                                             
                                       
   
                                  -                                             	                                           2                                                           	                                                                                                         	   &   	      '      
   
      	         
            C   0               	   
                              	                                                      	         $   E   +      4   1      (   /      (   ¯   t      ,   .       1   ,   x                                                                                                                                                                 F                                                                          	                                                                                                                                                                                                                   
 
                               
             
 
            -               	               2                     	                                     	 & 	  '  
 
  	   
    C 0     	 
          	                  	   $ E +  4 1  ( /  ( ¯ t  , .   1 , x   •×  —ƒ  —£  —ñ  �¿  ¤‘  ­MÍ'%ÄÞ�¢! €ÃÌÄ”¨K©Þl´ê«ÞA†âíŒÃ	¯#Æ$l%~%î"cµf1yøÓã7Åfõ#7€Š²¿”jû u ;%ÐJ	‘	ÇÓ+®¹¬Ÿ!{åw¶‹‹ `½Óå�;ƒŽÌ	 ] ‰ ì [	°{ÔåçÐJ	j	‘	î	
Ô
ÁÇÓÖ;�èZ–£ÏØ;ƒ¶g¡ý+XŠ®·Ž—½7;—óÿd³¹Ž¬ÍÏù2Ÿ!##_%Ñ% 
€Uë´“ò
ÆîK"“Ï
ÆîÌ!"“Æî"cå	µfç{ !¨!i¨Èu%ï�RÔÚ"
¸Á‰ÓY½ÁÄJØÈ¹Ì®ŠØÈ^#õÌàÍ_qÍ •ßÌW�Ëg­ÌË
5
-Ëß¨ Ã
#Ën¯"Â#‰44Ëg Ø"L=¢ëõ*	}	7
ƒu�´_ëöíÚÊÓ¦:!•O8 ˆ ¿M
((«)}¨O{J"ÏØ%¾ Ú~ZÀ5!) ºLÑtŒÎ"’ 7Ž•œ¶Cù[f‰|l#ÅÙ„#¿õ*	}	7
Ñ
ƒ(«Ùu¯�´þG¥
ç“%ß)è°
bÄIì{ÐY$Ä{Y$Åá3 n ¾k!@îr)ª£*~Ä]5{Y$Î×)è°
.¾PIìY£~æ$¹¿(«ï
C®˜Î
{½@ˆËÂ$>ÊíÈ:é!¢	hgÌzˆ„âoÅ%!Ë#Š$ ¿Q(«ýÄŸ$¡sµÞˆ <¿ËÌM
A(B×$l(‚«½È)}¨OÿZf{¢ð Ã!ì!J"N%u%ÍÌ
¬œ6Á!
] ª#)%¼#$˜’§ 	NÌÓ!9Î	¢� $œ{$a<mÆä	\pT×/!­PhŸ hã@i‰Š,¹ê%#k‹çç‘´½îDó$rÜÚÊó 2$Å$*!Ûëà!›³ó;Æ!ñ 	 åÐÓ-žÛyº)qc$ÎÖ8¹N“yÑ”˜ºÊ’ § B$ê%‘ÛÏ
ä	Tã‹‘�)rè°
.)ª£b¾*~ÄP]Iì5Y{ÐY$VÊÓÃ"“%{$amä	T/ã@¹‹‘îò •0„< Y ñ {Wÿ	ÆµÐ‘	½Ê~ŸÏ-@uˆkŠÀ , ²7@“ìórvŠ;¨!/#_%„%Ú%ä	\Tãi,#k‹ç‘(ã\{½û7@ˆ“%Î	’¡ø×%sµÞU”¥¥"±"##ƒoÙ	å0®] ºJ£ñÓq Bc� ,ç/‚×‹"ž"'}!¹$K%v
ûl³ÈÒÝav€‹)è°
IìÍÌ
‰|¬œ6lÁ!#Å
Ù„#r¼¿	*+s‡#eK™Eî*#û#¶CBŠâe	ö(åãëöíU�É©P(_C	•
»
åGmîãëöíÄŸÖç{q"Y$L î�OŠû
1šWºõ£Ú!Ê8ûÏ5ÓÀ!Ã"#`#Â#$$ª$ãçz"Ð$Õ$ó;: ½,B×çlt�J#?$Oav³ûº`S¾›!á: ltëöíG]¥¼á
 ª##$)%rôÆ[Ë° ë%eM Y2	žx€U	¥	Æ	ù	V°Ê`Å8 &y} 1ÔôÞå@û
,0Š²×%”Õ ¥"#]#“ùØÈV¦²"#Ž $œçÌ<¤Õ–	Ë
pLØJ1®iŸ!XiŠ¨Ìê5‘Š¤?çEó$ %y%
)
“OÙÜ”ÍÔ!÷ýÏ
	9×9 q†»¾TpÐÜ{£ôJMè“ûÂDD�ðkÓ`©îv ä!,#ú#ý#>$á%ó%ÌÖÏ�	h®±B•�Í;j¶‡¨ÌÛ1‰ª¯ �!ž!í!!¯†%¥ a!$)è	Tlí˜[É!¬"¥ágmL= Ìh•Í;‡Û¯ �!9 q†»¾Ü{ô“ûÂkÓ`©îä!>$á%ó%TpÐ£JMèDD�ðv ,#ú#ý#Õ
=-)7¸f­W…¨Iÿ‘Ìöœ›X w!ó!?K{Ì;•ã@&%y� $®#9 × ¹¼q†»¾TpÐÓÜ{£ÞôJM«ž¡è+Ž“®û\ÂD4D�éðü3kÓ¹`©ÊØî2 v ‘ ä!,#ú#ý#>$Í$h%Þ%á%ó%Ž $œçÌ<¤Õ–	Ë
pLØJ!1®iŸ¯!XiŠ†¨Ìê%5‘¥Š¤?çE a!$ó$ %y%aåÿ�tƒæDŒäòN ë	Çqh !<%Q &«à”È :ø‘H!s!é!#£	iŽhðÍê{@ ô#$§j‹”§W v!+"Ö
:�‘.œ¹gf˜•�[& Îf`râÆB"è"�$Ëß°Ë	´	\
L²‘ò»e¥†ÄvJ˜õKÆ@	‡	™
¿
O^*kÂÓiÈ¡¼a d"�#s%º%˜8Î•!f�Ê`rwâ[Æ& B"è"�$^ ¨»KL�—¤òÔñkr õ#$ü%ÎBcÇß`� è!Q"æ"ò‘�
Ñ
¯Ô½þd‰éÿJÚì".%  Ñë’	J§¼{³lÁ-F�³¿Aæ;Uj‹Y qsÜR°Û. ƒùÙ™â
ø
ŠùZÆ¨ FÔê"!$­%ø%ñC9>ê‚Ä $KÕtw&3!"/%¹™ù%/Ç³Ã

@<Í#á\¸Åi' Ÿ ª h"ï"u#Ö$$%"O1R¹ÇÞâ™&=¡!©MüÛê²~ „˜´ x#®$K�Šº	n
aì¼¼¥ÑàlDMà7Kçó‡C Ü !a"|#ð#/$ 2•0ßäçï
C;Þ®„n¼¯"Ä{™Ïÿ,/ó!
#¢# Ô"ø%ƒù
ø
Fê!$�ì¼¥àlD7óC !. Ù™âŠùZÆ¨­%× ¹¼ÓÞ«ž¡+Ž®\4éü3¹ÊØ2 ‘ Í$h%Þ%^ ÎB¨»KcL�—¤òÔñÇß`kr � è!Q"æ"õ#$ü%ûÖ©ï±þ -"0 Q›�q‡“"ÏU j" $ ò 
‚·LŠè}£ºüþ-n�Ã!A‹¯è¿×ëcŸëØ!"#g% ! Ø®½¶ÒÞì“	w
ÔßKrüm©¬¨´½ÉÓÞ|Ÿ¢´Ð,m�¯Â.@GpŠž´À5Bç4<Vbkw�ŒºŒ÷Ï ¿ C#Ó#Ã%Ø%ß%Y À  Cq¹î	
Œ
sèýÜ.4RX€\(°ÙéüÊØÛ 2Ÿì‘ ~!Ù!L#A$º$Í$L%h%
&•.›�IV	o	¦	Ç	ú	—
|W…±Ëa’PÆ÷9 ; { Ú ø*VéÐ‚X8Êª±
o~9�¢zªÇQçü}JmíÕOÉuLM¹ ø \!e#f$”$î$Ð% # d ñ ó À	„ÝRÔ$M‰p»ý)o‘¬þ "BŒÅÖ®§ÀÍÖìžê;!˜!!#Ž#<$ $ã$ñ%ö8Ž·@JÒwü
àAûQzMïZ!©"¦#™“-<Â1Æ
¢Ëî‹ù�³ØÈf&t¶ßV“q•¦¨ Ö è ="¦"²" ###^#* šê» ÖÏ�	¯MM±ÒB
>Žju�£¶Ï¨Ìëþ2²¸Áì#‰«9“ ž!î!¸#j%|%‡%¤ ¨!`œ¯ÛËC‡´%¦ô< ô b!¥!Ç![#$3$Æ$ã G]|¾©	å	¾UaÉü8ÂîÏ’¿A‰äXr˜áYŒ‰B’Ô] � Ï Ù Ä"”%* ; { ‘ ¦ Ú ø"*2KVéþHšÐêÿ‚»X Ó8Ê=cÖªÏØ�	±
 /f}ªÍÀ)3o¥¯R~ÆÏCMl´ºÕ9M�¢±Ýzª¾ÒBÇ$lç
2>DQfŽçju‰�£¶¿Ï^c£m¨üQV}�Ìëöþ2JmzœåíõÕIO•²¸ÁÉàì#u‰«>LZ«9M“ ¹ ø !\!ž!§!«!Å!î!ú!e#‚#­#¸#ù#H$a$f$”$î$P%j%|%‡%Ð%ã%� ¬ Ý ã -G]|¾®º¼ÁBDÚôöüµ2EGS‰ds¾		e	g	ž	©	±	å	¦
¨
ë
œ¾ßU�ÕöøaœÉóü8fÂîÊÏ’¿8A€‰¡äüXr˜á&9Yjƒ›Œ‰¡ÙB’ª#ÈÔä ] n { � Ï Ù !("x"z"|"Ä"Ú#—$%T%”%ö	+	~	Ï	8
EÅ„v¸‚µÂõ25£ÎÚÁ6!Ö"8 /BzÞ`9ºí^O¼”Ä:z˜•Ò:C‚‹þJê–ãÅ¨Ò¬_|p È ‰!ã!2#Z$m ¸ É ÷ Ó8z�²äñ
¨ÈºÁÛ'9L�Ž–�·r€K“ïgD ú�ý\g�k j!œ" þ M—U¼ç
¸sš÷¥=Mù{Ï 7Z½Ãý!N"Þ#"%åF’ÐÓÝÐÚaÈ 
¡\hæt>²äîúekR^‘	-0<{žÃ°¶ÛÞê‡­ {!é$&4 o ù !îHm–´â¼ÍÙ.o)0B¦ó¿¬ä!JVƒUÒáCT_kvØE!l!W"Œ"š"Ÿ"Ó"; G { Ú �ø*VéÐ‚ËX)8£Êk»ìªö	+	~	­	Ï	8
¡
±
æ
¢j~—oN~“9Ew�¢ÈSzªÅì^ÇZQ„ç<Lv|�ø¸õü}‚£µÂÝJRmíÕOÉõ2u5L~£ÎÚM†Á¹ ø 6!\!I"«"Ö"e#z#Ý#f$u$ˆ$”$À$î$!%Ð%ûð
¤â6\w†ì÷ÆØMyîG��1%  A     	¶  &M        &MÁDÏÖsWÏ»üÌ ñ­¥�87
¿L!3\Ö–!);{iÿC©Þ¦:ðì˜¢ Z!0eÖ­Ýtæa§=LÍi·•uS�8ÔébK¦5žè’è×`—JMv>�AU7ÈØñ �IB	ÆÈúitp.� q}áhóhÑ¢jR>NÆvWîà¤}„øîàåäýã¥>ÖTèú:Ô’"?.(ˆ{‚^Doíê’Ò U»“H3ñt¡S_D»±Í°èáLÍÃQ@0Ç‚sÕZ¡äÁED`#2ÒiL E¤lÆUk
Pô TUY•¥]™&S@NEÆ³J[àø¤Wm8‘ÕžÓ�}¿­ÑEéi™gI5þæˆ�µA‘TwÔêGQ‘UÕIGß7ML<áºÑøA„ZR™•™RIU¡}¤MýõQH#DEUH�N¢¾€®€M4Þ`3Lµ‡8p�GZh¸&HÓMZŽ9¡×M¸£^Ý. ÛSNô5ø8�QŸ•×Nø¨ŽàUí¨ßxâ>�c’�%•»�tj·qøÑ¥Ö�Ôég{éhQlÕ}5E…ÔZh‘•€Ô¨�h*]—Vƒˆ&eZke†I:	
”Y*£‰êæHY�Ð­ö›*ÑýûõÿñÐüPYQBDGDtokH\±Âl)z¥QT  	Ò4Qd‘Ùd×GgW•�UÕ§U­V©±”è¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M²¤…òÑ³àG=½Î*‡ïdQßäO}‹…™¬	|Q„\a?hV}°QÆÂ8î÷
tý*ô"¬çAoH»H˜D"ðà‘Ak��ôcƒ€¸"b‹^oUuQU�„tc­´Ò!TM/E]¼jY"€»ö]|]áQ&©'êRº`�QQºQà¶µ@*¢²©ÁfÝHT–-Ó˜d`q‚!ãW¯–¹MSÔa>¡ÑTŠˆ8�va8ÀJë»IÀ†.¶ÿSÚÐ>£ê)Ú�m3Kºˆ¡¶•´ÊÙ›Ö<_”ÝdÖGJEE Q¨ –ô êm –Mà¨uµ	%˜ ^ Œ­6–ôö8óô4ÞtM(a€Ø¤ôôÑ}-ÛY}WÔ5ØÑ=_Cë
àV^é’å®ÓZ
 m6õ�ÀÒ
¥� íM�®Rëƒ€�Qö!¦Tm‡lyÔq‡S±ÒÂM‚P1â·À �Ûà±81É\=Ô­2/@Äcßœ58…+n7ßN?#ø?²‡ç�#­<çü}ØSMÇ®µ/=�Årô7Û®µ5çQüÏ…+1ßà…ólN±ëPAÃ]¾ ÝN�y×OuË™¶M\6Ô!zÔo=×lAÃL�;<}çÓEßý°Çò8ãñ×Þ~KÏá5GýqÌmØ/Ž¬‡Ï<Þì6‰Ô—¼J\ËH,ˆ"-) T¿ÐïôÐÑI�ScnÖ’Ó_B T¿ÐïôÐÑI�ScnÖ’Ó_d¿?åï?oÏù{ÏÒ¼ý®óýƒ‡–ª©©�ñ>ÃîšôRHAª��ê�U:}‡Ä�OÜ#>Ï1Ç/ô'7û<ó+<yÿ=Ì÷|±¼=Ûß�Eî¾ÜÌ°;°Ô"ÌpÏ0ƒ�÷<2Å0¬ñÌØ<J
ü8<Í5Ñ�Cïƒ<ßÔó ¯ƒß,2ìü}ô@¸3Ë#¿Á C3À#óëàGï3ü×ïúçÃ­òß<rÄ#ñAó7à�‡óSð�‡??Ü4ÏÇÅA3qÿclô±Ö×î�V¢	®—˜£¾©ÎbsŽ¥�¨›cš:ü˜:¶«¾ú&Vø(âî¦J ü¢Ð× È"7ˆ¨áÍ÷‚€6�}ø´ï«Àz³«ÿ¿š=Ãí³Ï�£óŽ@;(áÖ¼‚(°8rú½ƒŽÏˆ£ß�®3ËëŽrþ°þÖôÁúúüÃã�ãºó.³ë?´q,¿¨>ÿ>¬NÂ~¥Xzu8‘ºOz©TU �@å­ï×uŽª:'€»¸)Fá>;¸9yxéOµ•:>„Žµ$ˆ�ËŠ"à—‘ûGú¤´ˆnäÒI+bÛ¦šÒjeèÕ…›DHD¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¦Ø¸;“›óþ^óöüÿ—¼ý+ÏÚï?Ø8yjªš˜Ñóì>á9¯E$„ê¨ùÉýk?™!,šs3·ƒË*�rÈx_àÊ^–Ï£MÛÿ«=82Y1D?7ÆÏëõÒŒÓá`Å€s?‰0Í¥3‚
s4È|çz=3ù3ó¾¬êëúú»=ÊÏ¯òÔîVÀþ¤
ñ©#©Ã~ÇýUz@†
Ü’Û?†G
«?¨r�/rº–Hè¯W�uÞ×ù»šê†¦P£Æ¬>h)Ua8j]U©s©uY¥Î�jBê°b3ÿšŠš«•�­Y‰¼í„u—øec­ešPQ&k]«È³úkúª0Ú£ëâª;Es‚%QŽm(ÜH
vÅàn×L%èDªÏñEÊ¯Xa7ªT®9ˆ3	¦üšð5^ÂÏ6wší‚„žßKtU6éÁƒaÒQ˜Ò8ö_a!—xÃ×§®q°AñA9|ê¨Ýv b^Ï¡²€_áaOgÕÇ!ßq�‰5ƒ]rŒÕæ—ƒžžäëð×ZY0eJ4zã;ëÃƒ‚¦Ž[�<!2ìÂm£MàF	OïÉ²Jnw-y�…ÅdtláûxiM —‹GÔ©í™ž¤hjUëj¥©T�…ê¡–í‡Õoér«+Þ-òEQµAºèFúª¸Ì–ãŽ�]U_|çÓ**<Q9õîªñB^´Ñ3F}Yâºä‰<’�÷Þµ¸“|ùÙû;Ž[âŸH8ãÒe,‹§À {QÆJ|C4ÂÀáp ¬�Ý?N�1qùß„gçL„æS=Î¿T0t&ð”Ã@°]LÜ@N½ Ï‚n¦KâÀ«=û‡¡2Æª!ŽË@ù�UÏMsªœ+àú£ƒêŽÝŠêøÊoøkˆ|š|ú„„áŸ;<ÓÅÇÄÍ1-!1     à  #  F  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  2Œ<?xml version="1.0"?><Features nextValidID="31"><Feature recentID="30" name="AmpR" directionality="1" translationMW="31558.16" type="CDS" allowSegmentOverlaps="0" cleavageArrows="8079" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment name="signal sequence" range="8011-8079" color="#ccffcc" type="standard" translated="1"/><Segment range="8080-8871" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="29" name="EGFP" directionality="1" translationMW="26942.08" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="4168-4170" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="4171-4173" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="4174-4887" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="28" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="5276-5875" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="27" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5942-6530" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="26" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9042-9630" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="RIGHT"/></Q></Feature><Feature recentID="25" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7425-7880" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="RIGHT"/></Q></Feature><Feature recentID="24" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4937-5266" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2234-2537" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="RRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1366-1599" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="3' LTR (Î”U3)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6617-6850" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;self-inactivating&amp;nbsp;3' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="RSV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="294-520" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Rous sarcoma virus enhancer/promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2538-2741" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="521-701" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5117-5252" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7083-7218" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="HIV-1 Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="748-873" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of human immunodeficiency virus type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6922-7043" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="cPPT/CTS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2095-2211" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;central polypurine tract and central termination sequence of HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="AmpR promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7906-8010" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="lac promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-35" range="149-154" color="#ffffff" type="standard"/><Segment range="155-172" color="#ffffff" type="standard"/><Segment name="-10" range="173-179" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="CAP binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="113-134" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="T3 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="248-266" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T3 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7239-7257" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="187-203" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="M13 rev" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="211-227" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7267-7283" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="Factor Xa site" directionality="2" translationMW="473.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="6412" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6029" maxFusedRunOn="6029" detectionMode="exactProteinMatch"><Segment range="6413-6424" color="#cc99b2" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="3" name="linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4887" maxFusedRunOn="4887"><Segment range="4138-4152" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="2" name="linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4887" maxFusedRunOn="4887"><Segment range="4153-4167" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="1" name="TARDBP" directionality="1" translationMW="44741.00" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4887" maxFusedRunOn="4887"><Segment range="2824-4065" color="#ffcc00" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="0" name="3xFLAG" directionality="1" translationMW="2730.72" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4887" maxFusedRunOn="4887" detectionMode="exactProteinMatch"><Segment range="4066-4131" color="#cc99b2" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;three tandem FLAGÂ® epitope tags, followed by an enterokinase cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  @<Notes>
<UUID>405fb08c-114c-411e-be3e-1395441a7d06</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:9:52">2018.9.13</Created>
<LastModified UTC="1:7:20">2025.11.12</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Ã<StrandColors><TopStrand><ColorRange range="0..2816" colors="black"/><ColorRange range="2817..2822" colors="blue"/><ColorRange range="4065..4130" colors="orange"/><ColorRange range="4131..9804" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..2816" colors="black"/><ColorRange range="2817..2822" colors="blue"/><ColorRange range="4065..4130" colors="orange"/><ColorRange range="4131..9804" colors="black"/></BottomStrand></StrandColors>  @Ú     	¶  &P        &PÁDÏÖsWÏ»üÌ ñ­¥�87
¿L!3\Ö–!);{iÿC©Þ¦:ðì˜¢ Z!0eÖ­Ýtæa§=LÍi·•uS�8ÔébK¦5žè’è×`—JMv>�AU7ÈØñ �IB	ÆÈúitp.� q}áhóhÑ¢jR>NÆvWîà¤}„øîàåäýã¥>ÖTèú:Ô’"?.(ˆ{‚^Doíê’Ò U»“H3ñt¡S_D»±Í°èáLÍÃQ@0Ç‚sÕZ¡äÁED`#2ÒiL E¤lÆUk
Pô TUY•¥]™&S@NEÆ³J[àø¤Wm8‘ÕžÓ�}¿­ÑEéi™gI5þæˆ�µA‘TwÔêGQ‘UÕIGß7ML<áºÑøA„ZR™•™RIU¡}¤MýõQH#DEUH�N¢¾€®€M4Þ`3Lµ‡8p�GZh¸&HÓMZŽ9¡×M¸£^Ý. ÛSNô5ø8�QŸ•×Nø¨ŽàUí¨ßxâ>�c’�%•»�tj·qøÑ¥Ö�Ôég{éhQlÕ}5E…ÔZh‘•€Ô¨�h*]—Vƒˆ&eZke†I:	
”Y*£‰êæHY�Ð­ö›*ÑýûõÿñÐüPYQBDGDtokH\±Âl)z¥QT  	Ò4Qd‘Ùd×GgW•�UÕ§U­V©±”è¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M²¤…òÑ³àG=½Î*‡ïdQßäO}‹…™¬	|Q„\a?hV}°QÆÂ8î÷
tý*ô"¬çAoH»H˜D"ðà‘Ak��ôcƒ€¸"b‹^oUuQU�„tc­´Ò!TM/E]¼jY"€»ö]|]áQ&©'êRº`�QQºQà¶µ@*¢²©ÁfÝHT–-Ó˜d`q‚!ãW¯–¹MSÔa>¡ÑTŠˆ8�va8ÀJë»IÀ†.¶ÿSÚÐ>£ê)Ú�m3Kºˆ¡¶•´ÊÙ›Ö<_”ÝdÖGJEE Q¨ –ô êm –Mà¨uµ	%˜ ^ Œ­6–ôö8óô4ÞtM(a€Ø¤ôôÑ}-ÛY}WÔ5ØÑ=_Cë
àV^é’å®ÓZ
 m6õ�ÀÒ
¥� íM�®Rëƒ€�Qö!¦Tm‡lyÔq‡RNÄ CH C	6M@ÄŠß ‚?o‚ÄàpÇ%p÷R´È¼11�~pÔáþ­¸ß|58ü�àýþÈ}þœ24Ž´óŸñ÷aM7ºÔ¼ö?9ËÐLßnºÔp×�Gó<B¬@Ç‚Í±8P6Ç­Avøu8uç]=×.fØ<u4QpÛP…ëQ¼÷]±24ìñ÷ŸMöÃ?Èã�Ç_yøMý/?„ÕõÄS1·`¾8²<ð{°ØN'R\ðy)s,} ±þ Xˆ´¤�RÿC¿ÓCE&5M�»ZKM}�RÿC¿ÓCE&5M�»ZKM}’üÿ—¼ý¿?åï?Jóö»ÏöZª¦¦4CÄ<û¸NkÑI!:ª>Cª5Téöu?pŒû=üÇ¿Ð<œßìóÌ¬ñçü÷3ÝòÆð|÷lL|N5ºûs2ÀìvÃPˆ0qÃ<Â.ÜðDËÀ"³Äl?3`ñ(L+ðàó4×F¾ó|?POÌ‚¾|°Ë³ñ÷Ð5à4Ï,Žÿ�<LÏ �Ï¯�¼Ïó_¿ëœL;·Ë|ñË�ÅÌß‚6ÍOÂ>üÿqüÓ?ÍÇý�³ÒÇ[_¸uZˆThx&º^bŽú§9ˆÎ:–>¢mŽhëò`êÚ®ûè™[à£‹º˜A(ƒò‹C\ƒ ˆÞ"£‡5ÿÞ
 Ú=÷âÓ¾¯êÎ¯þþh÷¶Ï>>�Î8 ì8 7‡Zò¢ÀáËêö;>"�~¸Ì/®9ËúÃû[ÓëëóŽ<;Œ>ëÌºÏ¬þÑÄ°nþ <ûüø±;	ú”}aéÔ4âFé=ê¥QT –·¿\QÔn:¨èžîà¤}„øîàåäýã¥>ÖTèú:Ô’"?.(ˆ{‚^Doíê’Ò U»“I$­‹nškI©—£TVm!^Šš4é–
ueMdÛujÝYSjªÞ6¹(‚¢õ{mbn›bàîNoÏù{ÏÛóþ^óô¯?k¼ÿ`áåªªjcD<CÏ°û„æ½’Cª Cä'õ¬þd„²iÌÎÞ,ªuË!àƒ)ýz[>�7oþ¬ôàÉdÅüß?¯×J3O…ƒÌþ$Ã6”Ì0")ÌÓ!ó�èôÏäÏÎú³«¯ëêì÷+<:¿ËS¹[ ;ú�+Æ¤Ž§ûõUèi+rHlþ*¬þ¡Ê½ÊêY {¢½^×{_æì.kª™@j�°ù ¥U„á©uV¥ÌZ¥Õf—:u©7ªÀˆÌþj*j®Vvµf&ó¶Ö_á•Žµ–hUAD˜I­v¯"Ïé¯ê¨Ãj�¯Š¨íýÎT•F8Q´ #q )Û�»]0”o¡«?Å*½a„Þ©R¸æ Ì&›òhsÀÔM{<ÙÞk´Xz
{}-ÑTÛ§‡IDZcHãÙ}„†]ã^ž¸uÆÁÅåóª£uØ m‰{>†Ê|……=ŸWL‡}Æ>$ÖuÊ3Wš^^z{“¯Ã]idxÁ•(Ð}ëŒì?¯
š8enð„È³	¶�7€=%?¿&É)¹Üp´5æ‘Ñ³„d#íá¥6‚^-R§¶fz‘¡©W­ª–¥PnBoª†[¶U¿¥ÈJ¬¯xL´ÉFÕë¡êªã2[Ž:5uU}óŸL ¨¨ðmDç×º«Å	x:ÓDÍõgŠë’$òJCßzÔ^âMóçgìî9oŠ} ã�H1”².Ÿ�íG)ðeÓ…À²7tý:ÅÇç|^Ÿ�2˜EL÷:üPPÁÐ›ÂSÁt3q:ô W>	º™/‹¬÷î„Ë¨†;- æW=5Ì>ªp¯ƒêŽª;v+«ã)¿á® eòióê†|ìóO4Ä´„Ä    à  &  I  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  2¢<?xml version="1.0"?><Features nextValidID="31"><Feature recentID="0" name="3xFLAG" directionality="1" translationMW="2730.72" type="CDS" allowSegmentOverlaps="0" cleavageArrows="4131" consecutiveTranslationNumbering="1" maxRunOn="4134" maxFusedRunOn="4134" detectionMode="exactProteinMatch"><Segment range="4066-4131" color="#cc99b2" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;three tandem FLAGÂ® epitope tags, followed by an enterokinase cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="30" name="TARDBP" directionality="1" translationMW="44741.00" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4134" maxFusedRunOn="4134"><Segment range="2824-4065" color="#ffcc00" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="29" name="linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4890" maxFusedRunOn="4890"><Segment range="4156-4170" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="28" name="linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4890" maxFusedRunOn="4890"><Segment range="4141-4155" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="27" name="Factor Xa site" directionality="2" translationMW="473.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="6415" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6032" maxFusedRunOn="6032" detectionMode="exactProteinMatch"><Segment range="6416-6427" color="#cc99b2" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="26" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7270-7286" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="25" name="M13 rev" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="211-227" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="187-203" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="T7 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7242-7260" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="T3 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="248-266" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T3 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="CAP binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="113-134" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="lac promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-35" range="149-154" color="#ffffff" type="standard"/><Segment range="155-172" color="#ffffff" type="standard"/><Segment name="-10" range="173-179" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="AmpR promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7909-8013" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="cPPT/CTS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2095-2211" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;central polypurine tract and central termination sequence of HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6925-7046" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="HIV-1 Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="748-873" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of human immunodeficiency virus type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7086-7221" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5120-5255" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="521-701" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2538-2741" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="RSV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="294-520" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Rous sarcoma virus enhancer/promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="3' LTR (Î”U3)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6620-6853" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;self-inactivating&amp;nbsp;3' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="RRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1366-1599" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2234-2537" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4940-5269" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7428-7883" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="RIGHT"/></Q></Feature><Feature recentID="5" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9045-9633" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="RIGHT"/></Q></Feature><Feature recentID="4" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5945-6533" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="5279-5878" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="2" name="EGFP" directionality="1" translationMW="26942.08" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="4171-4173" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="4174-4176" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="4177-4890" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="1" name="AmpR" directionality="1" translationMW="31558.16" type="CDS" allowSegmentOverlaps="0" cleavageArrows="8082" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment name="signal sequence" range="8014-8082" color="#ccffcc" type="standard" translated="1"/><Segment range="8083-8874" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  A<Notes>
<UUID>405fb08c-114c-411e-be3e-1395441a7d06</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:9:52">2018.9.13</Created>
<LastModified UTC="7:41:39">2025.11.10</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  g<StrandColors><TopStrand><ColorRange range="0..2816" colors="black"/><ColorRange range="4065..4133" colors="orange"/><ColorRange range="4134..9807" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..2816" colors="black"/><ColorRange range="4065..4133" colors="orange"/><ColorRange range="4134..9807" colors="black"/></BottomStrand></StrandColors>  ><     	�  &        &ÁDÏÖsWÏ»üÌ ñ­¥�87
¿L!3\Ö–!);{iÿC©Þ¦:ðì˜¢ Z!0eÖ­Ýtæa§=LÍi·•uS�8ÔébK¦5žè’è×`—JMv>�AU7ÈØñ �IB	ÆÈúitp.� q}áhóhÑ¢jR>NÆvWîà¤}„øîàåäýã¥>ÖTèú:Ô’"?.(ˆ{‚^Doíê’Ò U»“H3ñt¡S_D»±Í°èáLÍÃQ@0Ç‚sÕZ¡äÁED`#2ÒiL E¤lÆUk
Pô TUY•¥]™&S@NEÆ³J[àø¤Wm8‘ÕžÓ�}¿­ÑEéi™gI5þæˆ�µA‘TwÔêGQ‘UÕIGß7ML<áºÑøA„ZR™•™RIU¡}¤MýõQH#DEUH�N¢¾€®€M4Þ`3Lµ‡8p�GZh¸&HÓMZŽ9¡×M¸£^Ý. ÛSNô5ø8�QŸ•×Nø¨ŽàUí¨ßxâ>�c’�%•»�tj·qøÑ¥Ö�Ôég{éhQlÕ}5E…ÔZh‘•€Ô¨�h*]—Vƒˆ&eZke†I:	
”Y*£‰êæHY�Ð­ö›*ÑýûõÿñÐüPYQBDGDtokH\±Âl)z¥QT  	Ò4Qd‘Ùd×GgW•�UÕ§U­V©±”è¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M²¤…òÑ³àG=½Î*‡ïdQßäO}‹…™¬	|Q„\a?hV}°QÆÂ8î÷
tý*ô"¬çAoH»H˜D"ðà‘Ak��ôcƒ€¸"b‹^oUuQU�„tc­´Ò!TM/E]¼jY"€»ö]|]áQ&©'êRº`�QQºQà¶µ@*¢²©ÁfÝHT–-Ó˜d`q‚!ãW¯–¹MSÔa>¡ÑTŠˆ8�va8ÀJë»IÀ†.¶ÿSÚÐ>£ê)Ú�m3Kºˆ¡¶•´ÊÙ›Ö<_”ÝdÖGJEE Q¨ –ô êm –Mà¨uµ	%˜ ^ Œ­6–ôö8óô4ÞtM(a€Ø¤ôôÑ}-ÛY}WÔ5ØÑ=_Cë
àV^é’å®ÓZ
 m6õ�ÀÒ
¥� íM�®Rëƒ€;0� �$Ø!5N+|ý¾�Ã•ÃÝJÓ"ðÄ@Æ=ùÃS‡øR¶ã}ðÔãò?ƒ÷û!÷ø~pÈÒ:ÓÎÇÝ…4ÜzëRóØüPç/A3}ºëPqÃ^uÌñR±þ_6Äá@Ûµ5ÛàÔàI×�t÷\¹›`ñÔÑEÃmB­FóÝvÄ4ÈÓ³ÇÞ}4]ÿÛpÿ#Ž?}çá7ô¼þT×LÆÝ‚øàJÈ|óÀmîÃ`q8�IpÁä¥Ì±ô‚Çø�b"Ò�"KýÿM˜Õ66íi-5ô"KýÿM˜Õ66íi-5öKóþ^óöüÿ—¼ý+ÏÚï?Ø8yjªš˜Ñóì>á9¯E$„ê¨ù¨ÕS§Ø|IÔýÂ3ì÷órÿ@òs³Ï2³ÇŸóÜÏwËÁóÝ±0-ñ8Ô^ëíÌË±ÛB ,ÁÇó0¸sÁ,S 0ŠÏ°üÍƒÄ¡0¯ÃƒÌÓ]>ø3ÍðýA?2
ø=òÃ.ÏÇß@Ô€Ó<²;ü0ñ3<?>¾~ó?Í~ÿ®q0ì:ß-óÇ,B?3~Ø5?øsóýÇóLü|T7ö6ÏKm~àiÕj!Q¡àšéyŠ;êœæ g8êXú‰¶9£¯Éƒ«j»ï¢eo‚Ž.êa¢Ê-r‚#xŠŽ×ÿx(h÷ß‹Nú¼«:¿ûù£Ü0^Û<øú?8àt°â€ÞkÈ"‹ S‡/«Ø8ìøŠ=øã0¾¸ç/ëíoL¯¯Ì>8ðî0û¯2ë>³ûGÁ»ú€óïóàZÄì'êQõ‡§PÓ‰¤÷ª•EP xZÞýqGQ¸ê£¢x»‚‘ônà»ƒ—�÷Ž”ûYS£èHëRHˆü¸¢!î	y¿´ªKH�VîM$’¶-ºi­&¦^�QXY´D„Az*hÓ¦X)Õ•5“mÕ«ueMª«xÚä¢
‹Õíµ‰ºm‹ƒ¹9¿?åï?oÏù{ÏÒ¼ý®óýƒ‡–ª©©�ñ>ÃîšôRHAª��ŸÖ³ù’É§3;x<²©×,‡€Uþ§õélú4Ý¿ú³Óƒ%“Có|lþ¿](Í>X3ø“ÚS0Àˆ §3L‡Îw£Ó?“?;êÎ®¿¯«³Ü¬ðêÿ-Nål ïê@¯’:œ7ìÕW¡¤`­É =³ødpª³ú‡(÷+©d�îŠõx]í›°¹®¨jeª<jÃæ‚•V†¥ÕZ—1j—Uš\éÖ¤ Þ« V#0_ù¨©ª¹YÚÕ˜›ÎØGY†V:ÖY¡Ua&µÚ¼‹?¦¿ª£ª>¾*£·ôW8!RUáFÒ€�Ä€§l^ítÂQ¾„J¬ÿ\ªõ†z¥Jã˜ƒ0šoÉ¡ÏQ5ì,ógy®Ñaè(Iíô·ESnœ6%i�#�eöwŒ=zzá×—Îª�×`¶%ìú(ðNö}\q2÷ø“X5×(Í^ix1yéîN¿u¥‘à`CT£A÷®3°þ¼88*há•¸Â .Ì&Ú4Þ ô`”þü›$¦çqÀÐ×˜\VGFÎ��·†”Ú	x´}JžÙ™êF†¥^¶ªZ•A¹Q¾ªnØ}Vþ—!*²½á2Ñÿ$UT®„oª«ŒÉn8èÕÕU÷Î}0‚¢£ÁµŸ^ê¯%àëM4gÕž+®H“É)}ëQ{‰7Ï�Ÿ³¸å¾)ôƒŽ= ÆRÈº|
µd§Á”3L, 
ÈÝÓôè�ñxF~tÈNa3Üëñ@ECBo	L4ÐDÍÄëÐ\ø&êd¾,
³ß¸z,j¢ì´ 0O˜\ô×0ú©Â¾ª8>¨íØ®¯Œ¦ÿ†¸�—É§Ï¨HNó³Í<\|LÓÒ      !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  0}<?xml version="1.0"?><Features recycledIDs="0" nextValidID="31"><Feature recentID="30" name="TARDBP" directionality="1" translationMW="44741.00" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4821" maxFusedRunOn="4821"><Segment range="2824-4065" color="#ffcc00" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="29" name="linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4821" maxFusedRunOn="4821"><Segment range="4087-4101" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="28" name="linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4821" maxFusedRunOn="4821"><Segment range="4072-4086" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="27" name="Factor Xa site" directionality="2" translationMW="473.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="6346" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="5963" maxFusedRunOn="5963" detectionMode="exactProteinMatch"><Segment range="6347-6358" color="#cc99b2" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="26" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7201-7217" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="25" name="M13 rev" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="211-227" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="187-203" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="T7 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7173-7191" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="T3 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="248-266" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T3 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="CAP binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="113-134" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="lac promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-35" range="149-154" color="#ffffff" type="standard"/><Segment range="155-172" color="#ffffff" type="standard"/><Segment name="-10" range="173-179" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="AmpR promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7840-7944" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="cPPT/CTS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2095-2211" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;central polypurine tract and central termination sequence of HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6856-6977" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="HIV-1 Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="748-873" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of human immunodeficiency virus type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7017-7152" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5051-5186" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="521-701" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2538-2741" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="RSV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="294-520" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Rous sarcoma virus enhancer/promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="3' LTR (Î”U3)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6551-6784" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;self-inactivating&amp;nbsp;3' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="RRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1366-1599" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2234-2537" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4871-5200" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7359-7814" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="RIGHT"/></Q></Feature><Feature recentID="5" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8976-9564" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="RIGHT"/></Q></Feature><Feature recentID="4" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5876-6464" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="5210-5809" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="2" name="EGFP" directionality="1" translationMW="26942.08" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="4102-4104" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="4105-4107" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="4108-4821" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="1" name="AmpR" directionality="1" translationMW="31558.16" type="CDS" allowSegmentOverlaps="0" cleavageArrows="8013" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment name="signal sequence" range="7945-8013" color="#ccffcc" type="standard" translated="1"/><Segment range="8014-8805" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  A<Notes>
<UUID>405fb08c-114c-411e-be3e-1395441a7d06</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:9:52">2018.9.13</Created>
<LastModified UTC="7:41:33">2025.11.10</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  <StrandColors><TopStrand><ColorRange range="0..2816" colors="black"/><ColorRange range="4065..9738" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..2816" colors="black"/><ColorRange range="4065..9738" colors="black"/></BottomStrand></StrandColors>  >;     	œ  &        &ÁDÏÖsWÏ»üÌ ñ­¥�87
¿L!3\Ö–!);{iÿC©Þ¦:ðì˜¢ Z!0eÖ­Ýtæa§=LÍi·•uS�8ÔébK¦5žè’è×`—JMv>�AU7ÈØñ �IB	ÆÈúitp.� q}áhóhÑ¢jR>NÆvWîà¤}„øîàåäýã¥>ÖTèú:Ô’"?.(ˆ{‚^Doíê’Ò U»“H3ñt¡S_D»±Í°èáLÍÃQ@0Ç‚sÕZ¡äÁED`#2ÒiL E¤lÆUk
Pô TUY•¥]™&S@NEÆ³J[àø¤Wm8‘ÕžÓ�}¿­ÑEéi™gI5þæˆ�µA‘TwÔêGQ‘UÕIGß7ML<áºÑøA„ZR™•™RIU¡}¤MýõQH#DEUH�N¢¾€®€M4Þ`3Lµ‡8p�GZh¸&HÓMZŽ9¡×M¸£^Ý. ÛSNô5ø8�QŸ•×Nø¨ŽàUí¨ßxâ>�c’�%•»�tj·qøÑ¥Ö�Ôég{éhQlÕ}5E…ÔZh‘•€Ô¨�h*]—Vƒˆ&eZke†I:	
”Y*£‰êæHY�Ð­ö›*ÑýûõÿñÐüPYQBDGDtokH\±Âl)z¥QT  	Ò4Qd‘Ùd×GgW•�UÕ§U­V©±”è¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M²¤…òÑ³àG=½Î*‡ïdQßäX¸YšÀ—ÅEÆö…gÛ`#Žïp§OÒ¯@2 :Îtô‹´‰„B/	¸ØÿF88[‚&(µæõQ—UXGF:ÛM!RDÒôUÛÆ¥’(¿`U×ÅÐ~j’~ E+¦Ðµ¥kT ê+*œa]Ô…IbÝ1Ù†F"5zùk”Õ=FêMH¨€3‰ÑaáŒ®»´œˆ`bëoõ=­ê>¢�¨Ñ†Ñ£4»¨�ziP[L­™½cÅùMÖMdt¤TR‚	oB Ð j	dÑ  N
€W[P’Y€‚…êÊÓioOaƒ�?CMçDÒ€&ŠOOMÒÐý°%—Õ}C]�Õô>°®eî™ îZà�5  ÓoXÐ, ªXêÔØå.¸8c±ÒÂM‚P1â·À �Ûà±81É\=Ô­2/@Äcßœ58…+n7ßN?#ø?²‡ç�#­<çü}ØSMÇ®µ/=�Årô7Û®µ5çQüÏ…+1ßà…ólN±ëPAÃ]¾ ÝN�y×OuË™¶M\6Ô!zÔo=×lAÃL�;<}çÓEßý°Çò8ãñ×Þ~KÏá5GýqÌmØ/Ž¬‡Ï<Þì6‰Ô—¼J\ËH,ˆ"-) T¿ÐïôÐÑI�ScnÖ’Ó_B T¿ÐïôÐÑI�ScnÖ’Ó_d¿?åï?oÏù{ÏÒ¼ý®óýƒ‡–ª©©�ñ>ÃîšôRHAª��ê�U:}‡Ä�OÜ#>Ï1Ç/ô'7û<ó+<yÿ=Ì÷|±¼=Ûß�Eî¾ÜÌ°;°Ô"ÌpÏ0ƒ�÷<2Å0¬ñÌØ<J
ü8<Í5Ñ�Cïƒ<ßÔó ¯ƒß,2ìü}ô@¸3Ë#¿Á C3À#óëàGï3ü×ïúçÃ­òß<rÄ#ñAó7à�‡óSð�‡??Ü4ÏÇÅA3qÿclô±Ö×î�V¢	®—˜£¾©ÎbsŽ¥�¨›cš:ü˜:¶«¾ú&Vø(âî¦J ü¢Ð× È"7ˆ¨áÍ÷‚€6�}ø´ï«Àz³«ÿ¿š=Ãí³Ï�£óŽ@;(áÖ¼‚(°8rú½ƒŽÏˆ£ß�®3ËëŽrþ°þÖôÁúúüÃã�ãºó.³ë?´q,¿¨>ÿ>¬NÂ~¥Xzu8‘ºOz©TU �@å­ï×uŽª:'€»¸)Fá>;¸9yxéOµ•:>„Žµ$ˆ�ËŠ"à—‘ûGú¤´ˆnäÒI+bÛ¦šÒjeèÕ…›DHD¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¦Ø¸;“›óþ^óöüÿ—¼ý+ÏÚï?Ø8yjªš˜Ñóì>á9¯E$„ê¨ùÉýk?™!,šs3·ƒË*�rÈx_àÊ^–Ï£MÛÿ«=82Y1D?7ÆÏëõÒŒÓá`Å€s?‰0Í¥3‚
s4È|çz=3ù3ó¾¬êëúú»=ÊÏ¯òÔîVÀþ¤
ñ©#©Ã~ÇýUz@†
Ü’Û?†G
«?¨r�/rº–Hè¯W�uÞ×ù»šê†¦P£Æ¬>h)Ua8j]U©s©uY¥Î�jBê°b3ÿšŠš«•�­Y‰¼í„u—øec­ešPQ&k]«È³úkúª0Ú£ëâª;Es‚%QŽm(ÜH
vÅàn×L%èDªÏñEÊ¯Xa7ªT®9ˆ3	¦üšð5^ÂÏ6wší‚„žßKtU6éÁƒaÒQ˜Ò8ö_a!—xÃ×§®q°AñA9|ê¨Ýv b^Ï¡²€_áaOgÕÇ!ßq�‰5ƒ]rŒÕæ—ƒžžäëð×ZY0eJ4zã;ëÃƒ‚¦Ž[�<!2ìÂm£MàF	OïÉ²Jnw-y�…ÅdtláûxiM —‹GÔ©í™ž¤hjUëj¥©T�…ê¡–í‡Õoér«+Þ-òEQµAºèFúª¸Ì–ãŽ�]U_|çÓ**<Q9õîªñB^´Ñ3F}Yâºä‰<’�÷Þµ¸“|ùÙû;Ž[âŸH8ãÒe,‹§À {QÆJ|C4ÂÀáp ¬�Ý?N�1qùß„gçL„æS=Î¿T0t&ð”Ã@°]LÜ@N½ Ï‚n¦KâÀ«=û‡¡2Æª!ŽË@ù�UÏMsªœ+àú£ƒêŽÝŠêøÊoøkˆ|š|ú„„áŸ;<ÓÅÇÄÍ1-!1     þ  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  0}<?xml version="1.0"?><Features recycledIDs="0" nextValidID="31"><Feature recentID="30" name="TARDBP" directionality="1" translationMW="44741.00" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4815" maxFusedRunOn="4815"><Segment range="2818-4059" color="#ffcc00" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="29" name="linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4815" maxFusedRunOn="4815"><Segment range="4081-4095" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="28" name="linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4815" maxFusedRunOn="4815"><Segment range="4066-4080" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="27" name="Factor Xa site" directionality="2" translationMW="473.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="6340" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="5957" maxFusedRunOn="5957" detectionMode="exactProteinMatch"><Segment range="6341-6352" color="#cc99b2" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="26" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7195-7211" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="25" name="M13 rev" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="211-227" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="187-203" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="T7 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7167-7185" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="T3 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="248-266" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T3 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="CAP binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="113-134" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="lac promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-35" range="149-154" color="#ffffff" type="standard"/><Segment range="155-172" color="#ffffff" type="standard"/><Segment name="-10" range="173-179" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="AmpR promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7834-7938" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="cPPT/CTS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2095-2211" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;central polypurine tract and central termination sequence of HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6850-6971" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="HIV-1 Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="748-873" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of human immunodeficiency virus type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7011-7146" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5045-5180" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="521-701" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2538-2741" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="RSV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="294-520" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Rous sarcoma virus enhancer/promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="3' LTR (Î”U3)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6545-6778" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;self-inactivating&amp;nbsp;3' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="RRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1366-1599" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2234-2537" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4865-5194" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7353-7808" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="RIGHT"/></Q></Feature><Feature recentID="5" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8970-9558" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="RIGHT"/></Q></Feature><Feature recentID="4" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5870-6458" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="5204-5803" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="2" name="EGFP" directionality="1" translationMW="26942.08" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="4096-4098" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="4099-4101" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="4102-4815" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="1" name="AmpR" directionality="1" translationMW="31558.16" type="CDS" allowSegmentOverlaps="0" cleavageArrows="8007" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment name="signal sequence" range="7939-8007" color="#ccffcc" type="standard" translated="1"/><Segment range="8008-8799" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  A<Notes>
<UUID>405fb08c-114c-411e-be3e-1395441a7d06</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:9:52">2018.9.13</Created>
<LastModified UTC="7:40:42">2025.11.10</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  <StrandColors><TopStrand><ColorRange range="0..2816" colors="black"/><ColorRange range="4059..9732" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..2816" colors="black"/><ColorRange range="4059..9732" colors="black"/></BottomStrand></StrandColors>  >Ð     	œ  &        &ÁDÏÖsWÏ»üÌ ñ­¥�87
¿L!3\Ö–!);{iÿC©Þ¦:ðì˜¢ Z!0eÖ­Ýtæa§=LÍi·•uS�8ÔébK¦5žè’è×`—JMv>�AU7ÈØñ �IB	ÆÈúitp.� q}áhóhÑ¢jR>NÆvWîà¤}„øîàåäýã¥>ÖTèú:Ô’"?.(ˆ{‚^Doíê’Ò U»“H3ñt¡S_D»±Í°èáLÍÃQ@0Ç‚sÕZ¡äÁED`#2ÒiL E¤lÆUk
Pô TUY•¥]™&S@NEÆ³J[àø¤Wm8‘ÕžÓ�}¿­ÑEéi™gI5þæˆ�µA‘TwÔêGQ‘UÕIGß7ML<áºÑøA„ZR™•™RIU¡}¤MýõQH#DEUH�N¢¾€®€M4Þ`3Lµ‡8p�GZh¸&HÓMZŽ9¡×M¸£^Ý. ÛSNô5ø8�QŸ•×Nø¨ŽàUí¨ßxâ>�c’�%•»�tj·qøÑ¥Ö�Ôég{éhQlÕ}5E…ÔZh‘•€Ô¨�h*]—Vƒˆ&eZke†I:	
”Y*£‰êæHY�Ð­ö›*ÑýûõÿñÐüPYQBDGDtokH\±Âl)z¥QT  	Ò4Qd‘Ùd×GgW•�UÕ§U­V©±”è¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M²¤…òÑ³àG=½Î*‡ïdQßäX¸YšÀ—ÅEÆö…gÛ`#Žïp§OÒ¯@2 :Îtô‹´‰„B/	¸ØÿF88[‚&(µæõQ—UXGF:ÛM!RDÒôUÛÆ¥’(¿`U×ÅÐ~j’~ E+¦Ðµ¥kT ê+*œa]Ô…IbÝ1Ù†F"5zùk”Õ=FêMH¨€3‰ÑaáŒ®»´œˆ`bëoõ=­ê>¢�¨Ñ†Ñ£4»¨�ziP[L­™½cÅùMÖMdt¤TR‚	oB Ð j	dÑ  N
€W[P’Y€‚…êÊÓioOaƒ�?CMçDÒ€&ŠOOMÒÐý°%—Õ}C]�Õô>°®eî™ îZà�5  ÓoXÐ, ªXêÔØå.¸8bNÄ CH C	6M@ÄŠß ‚?o‚ÄàpÇ%p÷R´È¼11�~pÔáþ­¸ß|58ü�àýþÈ}þœ24Ž´óŸñ÷aM7ºÔ¼ö?9ËÐLßnºÔp×�Gó<B¬@Ç‚Í±8P6Ç­Avøu8uç]=×.fØ<u4QpÛP…ëQ¼÷]±24ìñ÷ŸMöÃ?Èã�Ç_yøMý/?„ÕõÄS1·`¾8²<ð{°ØN'R\ðy)s,} ±þ Xˆ´¤�RÿC¿ÓCE&5M�»ZKM}�RÿC¿ÓCE&5M�»ZKM}’üÿ—¼ý¿?åï?Jóö»ÏöZª¦¦4CÄ<û¸NkÑI!:ª>Cª5Téöu?pŒû=üÇ¿Ð<œßìóÌ¬ñçü÷3ÝòÆð|÷lL|N5ºûs2ÀìvÃPˆ0qÃ<Â.ÜðDËÀ"³Äl?3`ñ(L+ðàó4×F¾ó|?POÌ‚¾|°Ë³ñ÷Ð5à4Ï,Žÿ�<LÏ �Ï¯�¼Ïó_¿ëœL;·Ë|ñË�ÅÌß‚6ÍOÂ>üÿqüÓ?ÍÇý�³ÒÇ[_¸uZˆThx&º^bŽú§9ˆÎ:–>¢mŽhëò`êÚ®ûè™[à£‹º˜A(ƒò‹C\ƒ ˆÞ"£‡5ÿÞ
 Ú=÷âÓ¾¯êÎ¯þþh÷¶Ï>>�Î8 ì8 7‡Zò¢ÀáËêö;>"�~¸Ì/®9ËúÃû[ÓëëóŽ<;Œ>ëÌºÏ¬þÑÄ°nþ <ûüø±;	ú”}aéÔ4âFé=ê¥QT –·¿\QÔn:¨èžîà¤}„øîàåäýã¥>ÖTèú:Ô’"?.(ˆ{‚^Doíê’Ò U»“I$­‹nškI©—£TVm!^Šš4é–
ueMdÛujÝYSjªÞ6¹(‚¢õ{mbn›bàîNoÏù{ÏÛóþ^óô¯?k¼ÿ`áåªªjcD<CÏ°û„æ½’Cª Cä'õ¬þd„²iÌÎÞ,ªuË!àƒ)ýz[>�7oþ¬ôàÉdÅüß?¯×J3O…ƒÌþ$Ã6”Ì0")ÌÓ!ó�èôÏäÏÎú³«¯ëêì÷+<:¿ËS¹[ ;ú�+Æ¤Ž§ûõUèi+rHlþ*¬þ¡Ê½ÊêY {¢½^×{_æì.kª™@j�°ù ¥U„á©uV¥ÌZ¥Õf—:u©7ªÀˆÌþj*j®Vvµf&ó¶Ö_á•Žµ–hUAD˜I­v¯"Ïé¯ê¨Ãj�¯Š¨íýÎT•F8Q´ #q )Û�»]0”o¡«?Å*½a„Þ©R¸æ Ì&›òhsÀÔM{<ÙÞk´Xz
{}-ÑTÛ§‡IDZcHãÙ}„†]ã^ž¸uÆÁÅåóª£uØ m‰{>†Ê|……=ŸWL‡}Æ>$ÖuÊ3Wš^^z{“¯Ã]idxÁ•(Ð}ëŒì?¯
š8enð„È³	¶�7€=%?¿&É)¹Üp´5æ‘Ñ³„d#íá¥6‚^-R§¶fz‘¡©W­ª–¥PnBoª†[¶U¿¥ÈJ¬¯xL´ÉFÕë¡êªã2[Ž:5uU}óŸL ¨¨ðmDç×º«Å	x:ÓDÍõgŠë’$òJCßzÔ^âMóçgìî9oŠ} ã�H1”².Ÿ�íG)ðeÓ…À²7tý:ÅÇç|^Ÿ�2˜EL÷:üPPÁÐ›ÂSÁt3q:ô W>	º™/‹¬÷î„Ë¨†;- æW=5Ì>ªp¯ƒêŽª;v+«ã)¿á® eòióê†|ìóO4Ä´„Ä      !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  1<?xml version="1.0"?><Features nextValidID="31"><Feature recentID="30" name="TARDBP" directionality="1" translationMW="44741.00" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2818-4062" color="#ffcc00" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="29" name="linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4818" maxFusedRunOn="4818"><Segment range="4084-4098" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="28" name="linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4818" maxFusedRunOn="4818"><Segment range="4069-4083" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="27" name="Factor Xa site" directionality="2" translationMW="473.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="6343" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="5960" maxFusedRunOn="5960" detectionMode="exactProteinMatch"><Segment range="6344-6355" color="#cc99b2" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="26" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7198-7214" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="25" name="M13 rev" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="211-227" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="187-203" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="T7 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7170-7188" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="T3 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="248-266" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T3 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="CAP binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="113-134" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="lac promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-35" range="149-154" color="#ffffff" type="standard"/><Segment range="155-172" color="#ffffff" type="standard"/><Segment name="-10" range="173-179" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="AmpR promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7837-7941" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="cPPT/CTS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2095-2211" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;central polypurine tract and central termination sequence of HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6853-6974" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="HIV-1 Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="748-873" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of human immunodeficiency virus type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7014-7149" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5048-5183" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="521-701" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2538-2741" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="RSV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="294-520" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Rous sarcoma virus enhancer/promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="3' LTR (Î”U3)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6548-6781" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;self-inactivating&amp;nbsp;3' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="RRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1366-1599" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2234-2537" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4868-5197" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7356-7811" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="RIGHT"/></Q></Feature><Feature recentID="5" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8973-9561" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="RIGHT"/></Q></Feature><Feature recentID="4" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5873-6461" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="5207-5806" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="2" name="EGFP" directionality="1" translationMW="26942.08" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="4099-4101" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="4102-4104" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="4105-4818" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="1" name="AmpR" directionality="1" translationMW="31558.16" type="CDS" allowSegmentOverlaps="0" cleavageArrows="8010" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment name="signal sequence" range="7942-8010" color="#ccffcc" type="standard" translated="1"/><Segment range="8011-8802" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="0" name="MCS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2812-4068" color="#99ccff" type="standard"/></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  A<Notes>
<UUID>405fb08c-114c-411e-be3e-1395441a7d06</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:9:52">2018.9.13</Created>
<LastModified UTC="7:40:34">2025.11.10</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  <StrandColors><TopStrand><ColorRange range="0..2816" colors="black"/><ColorRange range="4062..9735" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..2816" colors="black"/><ColorRange range="4062..9735" colors="black"/></BottomStrand></StrandColors>  <º     k  !D        !DÁDÏÖsWÏ»üÌ ñ­¥�87
¿L!3\Ö–!);{iÿC©Þ¦:ðì˜¢ Z!0eÖ­Ýtæa§=LÍi·•uS�8ÔébK¦5žè’è×`—JMv>�AU7ÈØñ �IB	ÆÈúitp.� q}áhóhÑ¢jR>NÆvWîà¤}„øîàåäýã¥>ÖTèú:Ô’"?.(ˆ{‚^Doíê’Ò U»“H3ñt¡S_D»±Í°èáLÍÃQ@0Ç‚sÕZ¡äÁED`#2ÒiL E¤lÆUk
Pô TUY•¥]™&S@NEÆ³J[àø¤Wm8‘ÕžÓ�}¿­ÑEéi™gI5þæˆ�µA‘TwÔêGQ‘UÕIGß7ML<áºÑøA„ZR™•™RIU¡}¤MýõQH#DEUH�N¢¾€®€M4Þ`3Lµ‡8p�GZh¸&HÓMZŽ9¡×M¸£^Ý. ÛSNô5ø8�QŸ•×Nø¨ŽàUí¨ßxâ>�c’�%•»�tj·qøÑ¥Ö�Ôég{éhQlÕ}5E…ÔZh‘•€Ô¨�h*]—Vƒˆ&eZke†I:	
”Y*£‰êæHY�Ð­ö›*ÑýûõÿñÐüPYQBDGDtokH\±Âl)z¥QT  	Ò4Qd‘Ùd×GgW•�UÕ§U­V©±”è¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M²¤…òÑ³àG=½Î*‡ïdQßäNLïÀo|.Ä CH C	6M@ÄŠß ‚?o‚ÄàpÇ%p÷R´È¼11�~pÔáþ­¸ß|58ü�àýþÈ}þœ24Ž´óŸñ÷aM7ºÔ¼ö?9ËÐLßnºÔp×�Gó<B¬@Ç‚Í±8P6Ç­Avøu8uç]=×.fØ<u4QpÛP…ëQ¼÷]±24ìñ÷ŸMöÃ?Èã�Ç_yøMý/?„ÕõÄS1·`¾8²<ð{°ØN'R\ðy)s,} ±þ Xˆ´¤�RÿC¿ÓCE&5M�»ZKM}�RÿC¿ÓCE&5M�»ZKM}’üÿ—¼ý¿?åï?Jóö»ÏöZª¦¦4CÄ<û¸NkÑI!:ª>Cª5Téöu?pŒû=üÇ¿Ð<œßìóÌ¬ñçü÷3ÝòÆð|÷lL|N5ºûs2ÀìvÃPˆ0qÃ<Â.ÜðDËÀ"³Äl?3`ñ(L+ðàó4×F¾ó|?POÌ‚¾|°Ë³ñ÷Ð5à4Ï,Žÿ�<LÏ �Ï¯�¼Ïó_¿ëœL;·Ë|ñË�ÅÌß‚6ÍOÂ>üÿqüÓ?ÍÇý�³ÒÇ[_¸uZˆThx&º^bŽú§9ˆÎ:–>¢mŽhëò`êÚ®ûè™[à£‹º˜A(ƒò‹C\ƒ ˆÞ"£‡5ÿÞ
 Ú=÷âÓ¾¯êÎ¯þþh÷¶Ï>>�Î8 ì8 7‡Zò¢ÀáËêö;>"�~¸Ì/®9ËúÃû[ÓëëóŽ<;Œ>ëÌºÏ¬þÑÄ°nþ <ûüø±;	ú”}aéÔ4âFé=ê¥QT –·¿\QÔn:¨èžîà¤}„øîàåäýã¥>ÖTèú:Ô’"?.(ˆ{‚^Doíê’Ò U»“I$­‹nškI©—£TVm!^Šš4é–
ueMdÛujÝYSjªÞ6¹(‚¢õ{mbn›bàîNoÏù{ÏÛóþ^óô¯?k¼ÿ`áåªªjcD<CÏ°û„æ½’Cª Cä'õ¬þd„²iÌÎÞ,ªuË!àƒ)ýz[>�7oþ¬ôàÉdÅüß?¯×J3O…ƒÌþ$Ã6”Ì0")ÌÓ!ó�èôÏäÏÎú³«¯ëêì÷+<:¿ËS¹[ ;ú�+Æ¤Ž§ûõUèi+rHlþ*¬þ¡Ê½ÊêY {¢½^×{_æì.kª™@j�°ù ¥U„á©uV¥ÌZ¥Õf—:u©7ªÀˆÌþj*j®Vvµf&ó¶Ö_á•Žµ–hUAD˜I­v¯"Ïé¯ê¨Ãj�¯Š¨íýÎT•F8Q´ #q )Û�»]0”o¡«?Å*½a„Þ©R¸æ Ì&›òhsÀÔM{<ÙÞk´Xz
{}-ÑTÛ§‡IDZcHãÙ}„†]ã^ž¸uÆÁÅåóª£uØ m‰{>†Ê|……=ŸWL‡}Æ>$ÖuÊ3Wš^^z{“¯Ã]idxÁ•(Ð}ëŒì?¯
š8enð„È³	¶�7€=%?¿&É)¹Üp´5æ‘Ñ³„d#íá¥6‚^-R§¶fz‘¡©W­ª–¥PnBoª†[¶U¿¥ÈJ¬¯xL´ÉFÕë¡êªã2[Ž:5uU}óŸL ¨¨ðmDç×º«Å	x:ÓDÍõgŠë’$òJCßzÔ^âMóçgìî9oŠ} ã�H1”².Ÿ�íG)ðeÓ…À²7tý:ÅÇç|^Ÿ�2˜EL÷:üPPÁÐ›ÂSÁt3q:ô W>	º™/‹¬÷î„Ë¨†;- æW=5Ì>ªp¯ƒêŽª;v+«ã)¿á® eòióê†|ìóO4Ä´„Ä    =  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  /Å<?xml version="1.0"?><Features nextValidID="30"><Feature recentID="29" name="linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3598" maxFusedRunOn="3598"><Segment range="2864-2878" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="28" name="linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3598" maxFusedRunOn="3598"><Segment range="2849-2863" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="27" name="Factor Xa site" directionality="2" translationMW="473.53" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5123" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="4740" maxFusedRunOn="4740" detectionMode="exactProteinMatch"><Segment range="5124-5135" color="#cc99b2" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="26" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5978-5994" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="25" name="M13 rev" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="211-227" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="187-203" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="T7 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5950-5968" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="T3 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="248-266" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T3 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="CAP binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="113-134" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="lac promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-35" range="149-154" color="#ffffff" type="standard"/><Segment range="155-172" color="#ffffff" type="standard"/><Segment name="-10" range="173-179" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="AmpR promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6617-6721" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="cPPT/CTS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2095-2211" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;central polypurine tract and central termination sequence of HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5633-5754" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="HIV-1 Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="748-873" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of human immunodeficiency virus type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5794-5929" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3828-3963" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="521-701" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2538-2741" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="RSV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="294-520" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Rous sarcoma virus enhancer/promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="3' LTR (Î”U3)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5328-5561" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;self-inactivating&amp;nbsp;3' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="RRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1366-1599" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2234-2537" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3648-3977" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6136-6591" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="RIGHT"/></Q></Feature><Feature recentID="5" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7753-8341" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="RIGHT"/></Q></Feature><Feature recentID="4" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4653-5241" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="PuroR" directionality="1" translationMW="21496.48" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3987-4586" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="2" name="EGFP" directionality="1" translationMW="26941.53" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="2879-2881" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="2882-2884" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="2885-3598" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="1" name="AmpR" directionality="1" translationMW="31557.36" type="CDS" allowSegmentOverlaps="0" cleavageArrows="6790" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment name="signal sequence" range="6722-6790" color="#ccffcc" type="standard" translated="1"/><Segment range="6791-7582" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="0" name="MCS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2812-2848" color="#99ccff" type="standard"/></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  A<Notes>
<UUID>405fb08c-114c-411e-be3e-1395441a7d06</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:9:52">2018.9.13</Created>
<LastModified UTC="7:40:31">2025.11.10</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  o<StrandColors><TopStrand><ColorRange range="0..2816" colors="black"/><ColorRange range="2817..2841" colors="light blue"/><ColorRange range="2842..8515" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..2816" colors="black"/><ColorRange range="2817..2841" colors="light blue"/><ColorRange range="2842..8515" colors="black"/></BottomStrand></StrandColors>  =X     s  !d        !dÁDÏÖsWÏ»üÌ ñ­¥�87
¿L!3\Ö–!);{iÿC©Þ¦:ðì˜¢ Z!0eÖ­Ýtæa§=LÍi·•uS�8ÔébK¦5žè’è×`—JMv>�AU7ÈØñ �IB	ÆÈúitp.� q}áhóhÑ¢jR>NÆvWîà¤}„øîàåäýã¥>ÖTèú:Ô’"?.(ˆ{‚^Doíê’Ò U»“H3ñt¡S_D»±Í°èáLÍÃQ@0Ç‚sÕZ¡äÁED`#2ÒiL E¤lÆUk
Pô TUY•¥]™&S@NEÆ³J[àø¤Wm8‘ÕžÓ�}¿­ÑEéi™gI5þæˆ�µA‘TwÔêGQ‘UÕIGß7ML<áºÑøA„ZR™•™RIU¡}¤MýõQH#DEUH�N¢¾€®€M4Þ`3Lµ‡8p�GZh¸&HÓMZŽ9¡×M¸£^Ý. ÛSNô5ø8�QŸ•×Nø¨ŽàUí¨ßxâ>�c’�%•»�tj·qøÑ¥Ö�Ôég{éhQlÕ}5E…ÔZh‘•€Ô¨�h*]—Vƒˆ&eZke†I:	
”Y*£‰êæHY�Ð­ö›*ÑýûõÿñÐüPYQBDGDtokH\±Âl)z¥QT  	Ò4Qd‘Ùd×GgW•�UÕ§U­V©±”è¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M²¤…òÑ³àG=½Î*‡ïdQßäNLçÁí¾4±ÌÀo|.Ä CH C	6M@ÄŠß ‚?o‚ÄàpÇ%p÷R´È¼11�~pÔáþ­¸ß|58ü�àýþÈ}þœ24Ž´óŸñ÷aM7ºÔ¼ö?9ËÐLßnºÔp×�Gó<B¬@Ç‚Í±8P6Ç­Avøu8uç]=×.fØ<u4QpÛP…ëQ¼÷]±24ìñ÷ŸMöÃ?Èã�Ç_yøMý/?„ÕõÄS1·`¾8²<ð{°ØN'R\ðy)s,} ±þ Xˆ´¤�RÿC¿ÓCE&5M�»ZKM}�RÿC¿ÓCE&5M�»ZKM}’üÿ—¼ý¿?åï?Jóö»ÏöZª¦¦4CÄ<û¸NkÑI!:ª>Cª5Téöu?pŒû=üÇ¿Ð<œßìóÌ¬ñçü÷3ÝòÆð|÷lL|N5ºûs2ÀìvÃPˆ0qÃ<Â.ÜðDËÀ"³Äl?3`ñ(L+ðàó4×F¾ó|?POÌ‚¾|°Ë³ñ÷Ð5à4Ï,Žÿ�<LÏ �Ï¯�¼Ïó_¿ëœL;·Ë|ñË�ÅÌß‚6ÍOÂ>üÿqüÓ?ÍÇý�³ÒÇ[_¸uZˆThx&º^bŽú§9ˆÎ:–>¢mŽhëò`êÚ®ûè™[à£‹º˜A(ƒò‹C\ƒ ˆÞ"£‡5ÿÞ
 Ú=÷âÓ¾¯êÎ¯þþh÷¶Ï>>�Î8 ì8 7‡Zò¢ÀáËêö;>"�~¸Ì/®9ËúÃû[ÓëëóŽ<;Œ>ëÌºÏ¬þÑÄ°nþ <ûüø±;	ú”}aéÔ4âFé=ê¥QT –·¿\QÔn:¨èžîà¤}„øîàåäýã¥>ÖTèú:Ô’"?.(ˆ{‚^Doíê’Ò U»“I$­‹nškI©—£TVm!^Šš4é–
ueMdÛujÝYSjªÞ6¹(‚¢õ{mbn›bàîNoÏù{ÏÛóþ^óô¯?k¼ÿ`áåªªjcD<CÏ°û„æ½’Cª Cä'õ¬þd„²iÌÎÞ,ªuË!àƒ)ýz[>�7oþ¬ôàÉdÅüß?¯×J3O…ƒÌþ$Ã6”Ì0")ÌÓ!ó�èôÏäÏÎú³«¯ëêì÷+<:¿ËS¹[ ;ú�+Æ¤Ž§ûõUèi+rHlþ*¬þ¡Ê½ÊêY {¢½^×{_æì.kª™@j�°ù ¥U„á©uV¥ÌZ¥Õf—:u©7ªÀˆÌþj*j®Vvµf&ó¶Ö_á•Žµ–hUAD˜I­v¯"Ïé¯ê¨Ãj�¯Š¨íýÎT•F8Q´ #q )Û�»]0”o¡«?Å*½a„Þ©R¸æ Ì&›òhsÀÔM{<ÙÞk´Xz
{}-ÑTÛ§‡IDZcHãÙ}„†]ã^ž¸uÆÁÅåóª£uØ m‰{>†Ê|……=ŸWL‡}Æ>$ÖuÊ3Wš^^z{“¯Ã]idxÁ•(Ð}ëŒì?¯
š8enð„È³	¶�7€=%?¿&É)¹Üp´5æ‘Ñ³„d#íá¥6‚^-R§¶fz‘¡©W­ª–¥PnBoª†[¶U¿¥ÈJ¬¯xL´ÉFÕë¡êªã2[Ž:5uU}óŸL ¨¨ðmDç×º«Å	x:ÓDÍõgŠë’$òJCßzÔ^âMóçgìî9oŠ} ã�H1”².Ÿ�íG)ðeÓ…À²7tý:ÅÇç|^Ÿ�2˜EL÷:üPPÁÐ›ÂSÁt3q:ô W>	º™/‹¬÷î„Ë¨†;- æW=5Ì>ªp¯ƒêŽª;v+«ã)¿á® eòióê†|ìóO4Ä´„Ä    ]   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  0+<?xml version="1.0"?><Features nextValidID="30"><Feature recentID="2" name="MCS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2818-2874" color="#99ccff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;multiple cloning site&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="29" name="AmpR" directionality="1" translationMW="31557.36" type="CDS" allowSegmentOverlaps="0" cleavageArrows="6822" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment name="signal sequence" range="6754-6822" color="#ccffcc" type="standard" translated="1"/><Segment range="6823-7614" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="28" name="EGFP" directionality="1" translationMW="26941.53" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="2911-2913" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="2914-2916" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="2917-3630" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="27" name="PuroR" directionality="1" translationMW="21496.48" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="4019-4618" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="26" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4685-5273" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="25" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7785-8373" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6168-6623" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3680-4009" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2234-2537" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="RRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1366-1599" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="3' LTR (Î”U3)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5360-5593" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;self-inactivating&amp;nbsp;3' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="RSV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="294-520" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Rous sarcoma virus enhancer/promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2538-2741" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="521-701" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3860-3995" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5826-5961" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="HIV-1 Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="748-873" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of human immunodeficiency virus type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5665-5786" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="cPPT/CTS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2095-2211" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;central polypurine tract and central termination sequence of HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="AmpR promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6649-6753" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="lac promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-35" range="149-154" color="#ffffff" type="standard"/><Segment range="155-172" color="#ffffff" type="standard"/><Segment name="-10" range="173-179" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="CAP binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="113-134" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T3 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="248-266" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T3 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="T7 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5982-6000" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="187-203" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="M13 rev" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="211-227" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6010-6026" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="Factor Xa site" directionality="2" translationMW="473.53" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5155" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="4772" maxFusedRunOn="4772" detectionMode="exactProteinMatch"><Segment range="5156-5167" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="1" name="linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3630" maxFusedRunOn="3630"><Segment range="2881-2895" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3630" maxFusedRunOn="3630"><Segment range="2896-2910" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>405fb08c-114c-411e-be3e-1395441a7d06</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:9:52">2018.9.13</Created>
<LastModified UTC="2:38:25">2025.6.3</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  o<StrandColors><TopStrand><ColorRange range="0..2816" colors="black"/><ColorRange range="2817..2873" colors="light blue"/><ColorRange range="2874..8547" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..2816" colors="black"/><ColorRange range="2817..2873" colors="light blue"/><ColorRange range="2874..8547" colors="black"/></BottomStrand></StrandColors>  =Y     u  !j        !jÁDÏÖsWÏ»üÌ ñ­¥�87
¿L!3\Ö–!);{iÿC©Þ¦:ðì˜¢ Z!0eÖ­Ýtæa§=LÍi·•uS�8ÔébK¦5žè’è×`—JMv>�AU7ÈØñ �IB	ÆÈúitp.� q}áhóhÑ¢jR>NÆvWîà¤}„øîàåäýã¥>ÖTèú:Ô’"?.(ˆ{‚^Doíê’Ò U»“H3ñt¡S_D»±Í°èáLÍÃQ@0Ç‚sÕZ¡äÁED`#2ÒiL E¤lÆUk
Pô TUY•¥]™&S@NEÆ³J[àø¤Wm8‘ÕžÓ�}¿­ÑEéi™gI5þæˆ�µA‘TwÔêGQ‘UÕIGß7ML<áºÑøA„ZR™•™RIU¡}¤MýõQH#DEUH�N¢¾€®€M4Þ`3Lµ‡8p�GZh¸&HÓMZŽ9¡×M¸£^Ý. ÛSNô5ø8�QŸ•×Nø¨ŽàUí¨ßxâ>�c’�%•»�tj·qøÑ¥Ö�Ôég{éhQlÕ}5E…ÔZh‘•€Ô¨�h*]—Vƒˆ&eZke†I:	
”Y*£‰êæHY�Ð­ö›*ÑýûõÿñÐüPYQBDGDtokH\±Âl)z¥QT  	Ò4Qd‘Ùd×GgW•�UÕ§U­V©±”è¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M²¤…òÑ³àG=½Î*‡ïdQßäNLçÁí¾4±Â|Àü÷Âì@Â4‚0“`„ÔA8­ð#öø,NrWu+L‹Ã1÷çNáJÛ�÷ÃS�Èþßì‡ßáùÃ#HëO9ÿvÓqë­KÏcñCœ½Íöë­AÇyÔ3Ä!JÄwø!|Û…lzÔp×o€7S�'^uÓÝræmƒÇSEµ^µÏuÛpÓ#NÏyôÑwÿl1ÃüŽ8üu÷Ÿ„ßÒóøMQÿ\E3vã�+!óÏ·»�Äâu%Ào’—2ÇÒâˆ‹J@ˆ/ô;ý44RcTØÛµ¤´×Ðˆ/ô;ý44RcTØÛµ¤´×Ù/Ïù{ÏÛóþ^óô¯?k¼ÿ`áåªªjcD<CÏ°û„æ½’Cª Cä:£UNŸañ'S÷Ï³ßÌqËýÉÍþÏ<ÊÏÏs=ß,oÏvÄÀ·ÄãQ{¯·3,Çl5€³3Ì Âà}ÏL±L Â+<FÃó6„Â¿3Mt`PûàÏ7ÃõüÈ+à÷Ë»?}P.LòÈïðHÃÄÌðüúøûÌÿ5ûþ¹ÄÃ°ë|·Ï±üP|Íø#aüÔü#áÏÏ÷Í3ññPLÜØÛ=,uµû�§U¨…F‡‚k¥æ(ïªs˜�œã©cê&ØæŽ¿&­ªï¾‰•¾
8»©„ˆ?(´5È2�â*8s_ýà £ß~-;êð¬êÿïæ�pÁ{lóãèüã�ÐÃŠxu¯ Š,N¾¯`ã³â(÷àkŒÀrúãœ¿¬?µ½0~¾¿0øãÃ¸Ãî¼Ë¬úÏíKïêÏ¿Ï�k°Ÿ©GÖ�CN$n“ÞªU@àP9k{õÅFãªŽ‰à.î
GÑ¸O€Nî^@_Þ:SíeN�¡#­I"#òâˆ‡¸%äFþÑþ©-"[¹4’JØ¶é¦´š™z5EafÑè©£N™`§VTÖM·V­Õ•6ª­ãk’ˆ*/W¶Ö&é¶.äæüÿ—¼ý¿?åï?Jóö»ÏöZª¦¦4CÄ<û¸NkÑI!:ª>@rZÏæHK&œÌíàòÊ§\²Wø2Ÿ×¥³èÓvÿêÏN–LQÍñ³úýt£4øX1`ÏâL3iLÃ ‚œÍ29Þ�LþLüï«:ºþ¾®Ïr³Ã«üµ;•°¿©¼jHêpß±ÿU^†�!‚·$€öÏá‘ÂªÏê KÜ®¥’º+Õà]wµþnÂæº¡©”¨ñ«š
UXN—Uj\Åª]Vis§Z�ƒz¬XŒÁæ¢¦ªågkVbo;aeþXëYf…TI„š×jò,þšþªŒ6¨úøªŽßÑ\à…ITc…J7�±xµÓ	Fú*³üQr«ÖMê•+ŽbÂi¿&‡<D×°³Í�æ»E‡ ¡'·ÒÝMºp`Øt”E¦4Ž=—ØHeÞ0õéë‡\l|PN_:ª7]€Ø—³èl Á8XSÙõqÄÈwÜcâM`×\£5y¥àÅç§¹:ü5Ö–G��R�Þ¸ÎÃúðàà©£†VàOL€»0›hÓxÑ‚Sûòl’›�Ç KC^`aqY8FB>ÞSh%âÑõ*{fg©•zÚ©jUä!Fú¨e»aõ[ú\„ªÊ÷„ËGü‘TmPnº¾ª®3%¸ã£WUWß9ôÂ
Š�ÔN}{ª¼P—ƒ­4LÑŸVx®¹"O$¤=÷­Eî$ß>v~Îã–ø§Ò8ôƒK"éð(Ôq’ŸPÍ0°8\ +#wOÓ L\~wÅáùÓ!9„TÏs¯Å	¼%0Ð,A7¯@sà›©’ø°*Ï~áèL±ªˆc²Ð Á>`UsÓ\Ãê§
ø>¨àú£·bº¾2›þâ_&Ÿ>¡!8gÎÏ4ñqñ3LKHLA    c   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  0+<?xml version="1.0"?><Features nextValidID="30"><Feature recentID="2" name="MCS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2818-2880" color="#99ccff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;multiple cloning site&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="29" name="AmpR" directionality="1" translationMW="31557.36" type="CDS" allowSegmentOverlaps="0" cleavageArrows="6828" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment name="signal sequence" range="6760-6828" color="#ccffcc" type="standard" translated="1"/><Segment range="6829-7620" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="28" name="EGFP" directionality="1" translationMW="26941.53" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="2917-2919" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="2920-2922" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="2923-3636" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="27" name="PuroR" directionality="1" translationMW="21496.48" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="4025-4624" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="26" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4691-5279" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="25" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7791-8379" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6174-6629" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3686-4015" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2234-2537" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="RRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1366-1599" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="3' LTR (Î”U3)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5366-5599" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;self-inactivating&amp;nbsp;3' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="RSV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="294-520" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Rous sarcoma virus enhancer/promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2538-2741" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="521-701" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3866-4001" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5832-5967" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="HIV-1 Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="748-873" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of human immunodeficiency virus type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5671-5792" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="cPPT/CTS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2095-2211" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;central polypurine tract and central termination sequence of HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="AmpR promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6655-6759" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="lac promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-35" range="149-154" color="#ffffff" type="standard"/><Segment range="155-172" color="#ffffff" type="standard"/><Segment name="-10" range="173-179" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="CAP binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="113-134" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T3 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="248-266" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T3 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="T7 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5988-6006" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="187-203" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="M13 rev" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="211-227" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6016-6032" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="Factor Xa site" directionality="2" translationMW="473.53" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5161" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="4778" maxFusedRunOn="4778" detectionMode="exactProteinMatch"><Segment range="5162-5173" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="1" name="linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3636" maxFusedRunOn="3636"><Segment range="2887-2901" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3636" maxFusedRunOn="3636"><Segment range="2902-2916" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ><Notes>
<UUID>405fb08c-114c-411e-be3e-1395441a7d06</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:9:52">2018.9.13</Created>
<LastModified UTC="2:38:6">2025.6.3</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  o<StrandColors><TopStrand><ColorRange range="0..2816" colors="black"/><ColorRange range="2817..2879" colors="light blue"/><ColorRange range="2880..8553" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..2816" colors="black"/><ColorRange range="2817..2879" colors="light blue"/><ColorRange range="2880..8553" colors="black"/></BottomStrand></StrandColors>  =[     v  !p        !pÁDÏÖsWÏ»üÌ ñ­¥�87
¿L!3\Ö–!);{iÿC©Þ¦:ðì˜¢ Z!0eÖ­Ýtæa§=LÍi·•uS�8ÔébK¦5žè’è×`—JMv>�AU7ÈØñ �IB	ÆÈúitp.� q}áhóhÑ¢jR>NÆvWîà¤}„øîàåäýã¥>ÖTèú:Ô’"?.(ˆ{‚^Doíê’Ò U»“H3ñt¡S_D»±Í°èáLÍÃQ@0Ç‚sÕZ¡äÁED`#2ÒiL E¤lÆUk
Pô TUY•¥]™&S@NEÆ³J[àø¤Wm8‘ÕžÓ�}¿­ÑEéi™gI5þæˆ�µA‘TwÔêGQ‘UÕIGß7ML<áºÑøA„ZR™•™RIU¡}¤MýõQH#DEUH�N¢¾€®€M4Þ`3Lµ‡8p�GZh¸&HÓMZŽ9¡×M¸£^Ý. ÛSNô5ø8�QŸ•×Nø¨ŽàUí¨ßxâ>�c’�%•»�tj·qøÑ¥Ö�Ôég{éhQlÕ}5E…ÔZh‘•€Ô¨�h*]—Vƒˆ&eZke†I:	
”Y*£‰êæHY�Ð­ö›*ÑýûõÿñÐüPYQBDGDtokH\±Âl)z¥QT  	Ò4Qd‘Ùd×GgW•�UÕ§U­V©±”è¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M²¤…òÑ³àG=½Î*‡ïdQßäNLçÁí¼ZãK'ÌÀo|.Ä CH C	6M@ÄŠß ‚?o‚ÄàpÇ%p÷R´È¼11�~pÔáþ­¸ß|58ü�àýþÈ}þœ24Ž´óŸñ÷aM7ºÔ¼ö?9ËÐLßnºÔp×�Gó<B¬@Ç‚Í±8P6Ç­Avøu8uç]=×.fØ<u4QpÛP…ëQ¼÷]±24ìñ÷ŸMöÃ?Èã�Ç_yøMý/?„ÕõÄS1·`¾8²<ð{°ØN'R\ðy)s,} ±þ Xˆ´¤�RÿC¿ÓCE&5M�»ZKM}�RÿC¿ÓCE&5M�»ZKM}’üÿ—¼ý¿?åï?Jóö»ÏöZª¦¦4CÄ<û¸NkÑI!:ª>Cª5Téöu?pŒû=üÇ¿Ð<œßìóÌ¬ñçü÷3ÝòÆð|÷lL|N5ºûs2ÀìvÃPˆ0qÃ<Â.ÜðDËÀ"³Äl?3`ñ(L+ðàó4×F¾ó|?POÌ‚¾|°Ë³ñ÷Ð5à4Ï,Žÿ�<LÏ �Ï¯�¼Ïó_¿ëœL;·Ë|ñË�ÅÌß‚6ÍOÂ>üÿqüÓ?ÍÇý�³ÒÇ[_¸uZˆThx&º^bŽú§9ˆÎ:–>¢mŽhëò`êÚ®ûè™[à£‹º˜A(ƒò‹C\ƒ ˆÞ"£‡5ÿÞ
 Ú=÷âÓ¾¯êÎ¯þþh÷¶Ï>>�Î8 ì8 7‡Zò¢ÀáËêö;>"�~¸Ì/®9ËúÃû[ÓëëóŽ<;Œ>ëÌºÏ¬þÑÄ°nþ <ûüø±;	ú”}aéÔ4âFé=ê¥QT –·¿\QÔn:¨èžîà¤}„øîàåäýã¥>ÖTèú:Ô’"?.(ˆ{‚^Doíê’Ò U»“I$­‹nškI©—£TVm!^Šš4é–
ueMdÛujÝYSjªÞ6¹(‚¢õ{mbn›bàîNoÏù{ÏÛóþ^óô¯?k¼ÿ`áåªªjcD<CÏ°û„æ½’Cª Cä'õ¬þd„²iÌÎÞ,ªuË!àƒ)ýz[>�7oþ¬ôàÉdÅüß?¯×J3O…ƒÌþ$Ã6”Ì0")ÌÓ!ó�èôÏäÏÎú³«¯ëêì÷+<:¿ËS¹[ ;ú�+Æ¤Ž§ûõUèi+rHlþ*¬þ¡Ê½ÊêY {¢½^×{_æì.kª™@j�°ù ¥U„á©uV¥ÌZ¥Õf—:u©7ªÀˆÌþj*j®Vvµf&ó¶Ö_á•Žµ–hUAD˜I­v¯"Ïé¯ê¨Ãj�¯Š¨íýÎT•F8Q´ #q )Û�»]0”o¡«?Å*½a„Þ©R¸æ Ì&›òhsÀÔM{<ÙÞk´Xz
{}-ÑTÛ§‡IDZcHãÙ}„†]ã^ž¸uÆÁÅåóª£uØ m‰{>†Ê|……=ŸWL‡}Æ>$ÖuÊ3Wš^^z{“¯Ã]idxÁ•(Ð}ëŒì?¯
š8enð„È³	¶�7€=%?¿&É)¹Üp´5æ‘Ñ³„d#íá¥6‚^-R§¶fz‘¡©W­ª–¥PnBoª†[¶U¿¥ÈJ¬¯xL´ÉFÕë¡êªã2[Ž:5uU}óŸL ¨¨ðmDç×º«Å	x:ÓDÍõgŠë’$òJCßzÔ^âMóçgìî9oŠ} ã�H1”².Ÿ�íG)ðeÓ…À²7tý:ÅÇç|^Ÿ�2˜EL÷:üPPÁÐ›ÂSÁt3q:ô W>	º™/‹¬÷î„Ë¨†;- æW=5Ì>ªp¯ƒêŽª;v+«ã)¿á® eòióê†|ìóO4Ä´„Ä    i   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  0+<?xml version="1.0"?><Features nextValidID="30"><Feature recentID="2" name="MCS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2818-2886" color="#99ccff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;multiple cloning site&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="29" name="AmpR" directionality="1" translationMW="31557.36" type="CDS" allowSegmentOverlaps="0" cleavageArrows="6834" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment name="signal sequence" range="6766-6834" color="#ccffcc" type="standard" translated="1"/><Segment range="6835-7626" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="28" name="EGFP" directionality="1" translationMW="26941.53" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="2923-2925" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="2926-2928" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="2929-3642" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="27" name="PuroR" directionality="1" translationMW="21496.48" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="4031-4630" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="26" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4697-5285" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="25" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7797-8385" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6180-6635" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3692-4021" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2234-2537" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="RRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1366-1599" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="3' LTR (Î”U3)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5372-5605" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;self-inactivating&amp;nbsp;3' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="RSV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="294-520" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Rous sarcoma virus enhancer/promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2538-2741" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="521-701" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3872-4007" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5838-5973" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="HIV-1 Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="748-873" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of human immunodeficiency virus type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5677-5798" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="cPPT/CTS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2095-2211" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;central polypurine tract and central termination sequence of HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="AmpR promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6661-6765" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="lac promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-35" range="149-154" color="#ffffff" type="standard"/><Segment range="155-172" color="#ffffff" type="standard"/><Segment name="-10" range="173-179" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="CAP binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="113-134" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T3 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="248-266" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T3 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="T7 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5994-6012" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="187-203" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="M13 rev" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="211-227" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6022-6038" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="Factor Xa site" directionality="2" translationMW="473.53" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5167" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="4784" maxFusedRunOn="4784" detectionMode="exactProteinMatch"><Segment range="5168-5179" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="1" name="linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3642" maxFusedRunOn="3642"><Segment range="2893-2907" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3642" maxFusedRunOn="3642"><Segment range="2908-2922" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>405fb08c-114c-411e-be3e-1395441a7d06</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:9:52">2018.9.13</Created>
<LastModified UTC="2:37:47">2025.6.3</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  o<StrandColors><TopStrand><ColorRange range="0..2816" colors="black"/><ColorRange range="2817..2885" colors="light blue"/><ColorRange range="2886..8559" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..2816" colors="black"/><ColorRange range="2817..2885" colors="light blue"/><ColorRange range="2886..8559" colors="black"/></BottomStrand></StrandColors>  =]     x  !v        !vÁDÏÖsWÏ»üÌ ñ­¥�87
¿L!3\Ö–!);{iÿC©Þ¦:ðì˜¢ Z!0eÖ­Ýtæa§=LÍi·•uS�8ÔébK¦5žè’è×`—JMv>�AU7ÈØñ �IB	ÆÈúitp.� q}áhóhÑ¢jR>NÆvWîà¤}„øîàåäýã¥>ÖTèú:Ô’"?.(ˆ{‚^Doíê’Ò U»“H3ñt¡S_D»±Í°èáLÍÃQ@0Ç‚sÕZ¡äÁED`#2ÒiL E¤lÆUk
Pô TUY•¥]™&S@NEÆ³J[àø¤Wm8‘ÕžÓ�}¿­ÑEéi™gI5þæˆ�µA‘TwÔêGQ‘UÕIGß7ML<áºÑøA„ZR™•™RIU¡}¤MýõQH#DEUH�N¢¾€®€M4Þ`3Lµ‡8p�GZh¸&HÓMZŽ9¡×M¸£^Ý. ÛSNô5ø8�QŸ•×Nø¨ŽàUí¨ßxâ>�c’�%•»�tj·qøÑ¥Ö�Ôég{éhQlÕ}5E…ÔZh‘•€Ô¨�h*]—Vƒˆ&eZke†I:	
”Y*£‰êæHY�Ð­ö›*ÑýûõÿñÐüPYQBDGDtokH\±Âl)z¥QT  	Ò4Qd‘Ùd×GgW•�UÕ§U­V©±”è¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M²¤…òÑ³àG=½Î*‡ïdQßäNLçÁíµ:Å®4±Â|Àü÷Âì@Â4‚0“`„ÔA8­ð#öø,NrWu+L‹Ã1÷çNáJÛ�÷ÃS�Èþßì‡ßáùÃ#HëO9ÿvÓqë­KÏcñCœ½Íöë­AÇyÔ3Ä!JÄwø!|Û…lzÔp×o€7S�'^uÓÝræmƒÇSEµ^µÏuÛpÓ#NÏyôÑwÿl1ÃüŽ8üu÷Ÿ„ßÒóøMQÿ\E3vã�+!óÏ·»�Äâu%Ào’—2ÇÒâˆ‹J@ˆ/ô;ý44RcTØÛµ¤´×Ðˆ/ô;ý44RcTØÛµ¤´×Ù/Ïù{ÏÛóþ^óô¯?k¼ÿ`áåªªjcD<CÏ°û„æ½’Cª Cä:£UNŸañ'S÷Ï³ßÌqËýÉÍþÏ<ÊÏÏs=ß,oÏvÄÀ·ÄãQ{¯·3,Çl5€³3Ì Âà}ÏL±L Â+<FÃó6„Â¿3Mt`PûàÏ7ÃõüÈ+à÷Ë»?}P.LòÈïðHÃÄÌðüúøûÌÿ5ûþ¹ÄÃ°ë|·Ï±üP|Íø#aüÔü#áÏÏ÷Í3ññPLÜØÛ=,uµû�§U¨…F‡‚k¥æ(ïªs˜�œã©cê&ØæŽ¿&­ªï¾‰•¾
8»©„ˆ?(´5È2�â*8s_ýà £ß~-;êð¬êÿïæ�pÁ{lóãèüã�ÐÃŠxu¯ Š,N¾¯`ã³â(÷àkŒÀrúãœ¿¬?µ½0~¾¿0øãÃ¸Ãî¼Ë¬úÏíKïêÏ¿Ï�k°Ÿ©GÖ�CN$n“ÞªU@àP9k{õÅFãªŽ‰à.î
GÑ¸O€Nî^@_Þ:SíeN�¡#­I"#òâˆ‡¸%äFþÑþ©-"[¹4’JØ¶é¦´š™z5EafÑè©£N™`§VTÖM·V­Õ•6ª­ãk’ˆ*/W¶Ö&é¶.äæüÿ—¼ý¿?åï?Jóö»ÏöZª¦¦4CÄ<û¸NkÑI!:ª>@rZÏæHK&œÌíàòÊ§\²Wø2Ÿ×¥³èÓvÿêÏN–LQÍñ³úýt£4øX1`ÏâL3iLÃ ‚œÍ29Þ�LþLüï«:ºþ¾®Ïr³Ã«üµ;•°¿©¼jHêpß±ÿU^†�!‚·$€öÏá‘ÂªÏê KÜ®¥’º+Õà]wµþnÂæº¡©”¨ñ«š
UXN—Uj\Åª]Vis§Z�ƒz¬XŒÁæ¢¦ªågkVbo;aeþXëYf…TI„š×jò,þšþªŒ6¨úøªŽßÑ\à…ITc…J7�±xµÓ	Fú*³üQr«ÖMê•+ŽbÂi¿&‡<D×°³Í�æ»E‡ ¡'·ÒÝMºp`Øt”E¦4Ž=—ØHeÞ0õéë‡\l|PN_:ª7]€Ø—³èl Á8XSÙõqÄÈwÜcâM`×\£5y¥àÅç§¹:ü5Ö–G��R�Þ¸ÎÃúðàà©£†VàOL€»0›hÓxÑ‚Sûòl’›�Ç KC^`aqY8FB>ÞSh%âÑõ*{fg©•zÚ©jUä!Fú¨e»aõ[ú\„ªÊ÷„ËGü‘TmPnº¾ª®3%¸ã£WUWß9ôÂ
Š�ÔN}{ª¼P—ƒ­4LÑŸVx®¹"O$¤=÷­Eî$ß>v~Îã–ø§Ò8ôƒK"éð(Ôq’ŸPÍ0°8\ +#wOÓ L\~wÅáùÓ!9„TÏs¯Å	¼%0Ð,A7¯@sà›©’ø°*Ï~áèL±ªˆc²Ð Á>`UsÓ\Ãê§
ø>¨àú£·bº¾2›þâ_&Ÿ>¡!8gÎÏ4ñqñ3LKHLA    o   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  0+<?xml version="1.0"?><Features nextValidID="30"><Feature recentID="2" name="MCS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2818-2892" color="#99ccff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;multiple cloning site&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="29" name="AmpR" directionality="1" translationMW="31557.36" type="CDS" allowSegmentOverlaps="0" cleavageArrows="6840" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment name="signal sequence" range="6772-6840" color="#ccffcc" type="standard" translated="1"/><Segment range="6841-7632" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="28" name="EGFP" directionality="1" translationMW="26941.53" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="2929-2931" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="2932-2934" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="2935-3648" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="27" name="PuroR" directionality="1" translationMW="21496.48" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="4037-4636" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="26" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4703-5291" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="25" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7803-8391" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6186-6641" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3698-4027" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2234-2537" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="RRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1366-1599" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="3' LTR (Î”U3)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5378-5611" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;self-inactivating&amp;nbsp;3' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="RSV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="294-520" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Rous sarcoma virus enhancer/promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2538-2741" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="521-701" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3878-4013" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5844-5979" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="HIV-1 Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="748-873" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of human immunodeficiency virus type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5683-5804" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="cPPT/CTS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2095-2211" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;central polypurine tract and central termination sequence of HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="AmpR promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6667-6771" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="lac promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-35" range="149-154" color="#ffffff" type="standard"/><Segment range="155-172" color="#ffffff" type="standard"/><Segment name="-10" range="173-179" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="CAP binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="113-134" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T3 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="248-266" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T3 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="T7 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6000-6018" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="187-203" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="M13 rev" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="211-227" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6028-6044" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="Factor Xa site" directionality="2" translationMW="473.53" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5173" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="4790" maxFusedRunOn="4790" detectionMode="exactProteinMatch"><Segment range="5174-5185" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="1" name="linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3648" maxFusedRunOn="3648"><Segment range="2899-2913" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3648" maxFusedRunOn="3648"><Segment range="2914-2928" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>405fb08c-114c-411e-be3e-1395441a7d06</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:9:52">2018.9.13</Created>
<LastModified UTC="2:37:35">2025.6.3</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  o<StrandColors><TopStrand><ColorRange range="0..2816" colors="black"/><ColorRange range="2817..2891" colors="light blue"/><ColorRange range="2892..8565" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..2816" colors="black"/><ColorRange range="2817..2891" colors="light blue"/><ColorRange range="2892..8565" colors="black"/></BottomStrand></StrandColors>  <ö     y  !|        !|ÁDÏÖsWÏ»üÌ ñ­¥�87
¿L!3\Ö–!);{iÿC©Þ¦:ðì˜¢ Z!0eÖ­Ýtæa§=LÍi·•uS�8ÔébK¦5žè’è×`—JMv>�AU7ÈØñ �IB	ÆÈúitp.� q}áhóhÑ¢jR>NÆvWîà¤}„øîàåäýã¥>ÖTèú:Ô’"?.(ˆ{‚^Doíê’Ò U»“H3ñt¡S_D»±Í°èáLÍÃQ@0Ç‚sÕZ¡äÁED`#2ÒiL E¤lÆUk
Pô TUY•¥]™&S@NEÆ³J[àø¤Wm8‘ÕžÓ�}¿­ÑEéi™gI5þæˆ�µA‘TwÔêGQ‘UÕIGß7ML<áºÑøA„ZR™•™RIU¡}¤MýõQH#DEUH�N¢¾€®€M4Þ`3Lµ‡8p�GZh¸&HÓMZŽ9¡×M¸£^Ý. ÛSNô5ø8�QŸ•×Nø¨ŽàUí¨ßxâ>�c’�%•»�tj·qøÑ¥Ö�Ôég{éhQlÕ}5E…ÔZh‘•€Ô¨�h*]—Vƒˆ&eZke†I:	
”Y*£‰êæHY�Ð­ö›*ÑýûõÿñÐüPYQBDGDtokH\±Âl)z¥QT  	Ò4Qd‘Ùd×GgW•�UÕ§U­V©±”è¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M²¤…òÑ³àG=½Î*‡ïdQßäNLçÁí±;S¬ZãK'ÌÀo|.Ä CH C	6M@ÄŠß ‚?o‚ÄàpÇ%p÷R´È¼11�~pÔáþ­¸ß|58ü�àýþÈ}þœ24Ž´óŸñ÷aM7ºÔ¼ö?9ËÐLßnºÔp×�Gó<B¬@Ç‚Í±8P6Ç­Avøu8uç]=×.fØ<u4QpÛP…ëQ¼÷]±24ìñ÷ŸMöÃ?Èã�Ç_yøMý/?„ÕõÄS1·`¾8²<ð{°ØN'R\ðy)s,} ±þ Xˆ´¤�RÿC¿ÓCE&5M�»ZKM}�RÿC¿ÓCE&5M�»ZKM}’üÿ—¼ý¿?åï?Jóö»ÏöZª¦¦4CÄ<û¸NkÑI!:ª>Cª5Téöu?pŒû=üÇ¿Ð<œßìóÌ¬ñçü÷3ÝòÆð|÷lL|N5ºûs2ÀìvÃPˆ0qÃ<Â.ÜðDËÀ"³Äl?3`ñ(L+ðàó4×F¾ó|?POÌ‚¾|°Ë³ñ÷Ð5à4Ï,Žÿ�<LÏ �Ï¯�¼Ïó_¿ëœL;·Ë|ñË�ÅÌß‚6ÍOÂ>üÿqüÓ?ÍÇý�³ÒÇ[_¸uZˆThx&º^bŽú§9ˆÎ:–>¢mŽhëò`êÚ®ûè™[à£‹º˜A(ƒò‹C\ƒ ˆÞ"£‡5ÿÞ
 Ú=÷âÓ¾¯êÎ¯þþh÷¶Ï>>�Î8 ì8 7‡Zò¢ÀáËêö;>"�~¸Ì/®9ËúÃû[ÓëëóŽ<;Œ>ëÌºÏ¬þÑÄ°nþ <ûüø±;	ú”}aéÔ4âFé=ê¥QT –·¿\QÔn:¨èžîà¤}„øîàåäýã¥>ÖTèú:Ô’"?.(ˆ{‚^Doíê’Ò U»“I$­‹nškI©—£TVm!^Šš4é–
ueMdÛujÝYSjªÞ6¹(‚¢õ{mbn›bàîNoÏù{ÏÛóþ^óô¯?k¼ÿ`áåªªjcD<CÏ°û„æ½’Cª Cä'õ¬þd„²iÌÎÞ,ªuË!àƒ)ýz[>�7oþ¬ôàÉdÅüß?¯×J3O…ƒÌþ$Ã6”Ì0")ÌÓ!ó�èôÏäÏÎú³«¯ëêì÷+<:¿ËS¹[ ;ú�+Æ¤Ž§ûõUèi+rHlþ*¬þ¡Ê½ÊêY {¢½^×{_æì.kª™@j�°ù ¥U„á©uV¥ÌZ¥Õf—:u©7ªÀˆÌþj*j®Vvµf&ó¶Ö_á•Žµ–hUAD˜I­v¯"Ïé¯ê¨Ãj�¯Š¨íýÎT•F8Q´ #q )Û�»]0”o¡«?Å*½a„Þ©R¸æ Ì&›òhsÀÔM{<ÙÞk´Xz
{}-ÑTÛ§‡IDZcHãÙ}„†]ã^ž¸uÆÁÅåóª£uØ m‰{>†Ê|……=ŸWL‡}Æ>$ÖuÊ3Wš^^z{“¯Ã]idxÁ•(Ð}ëŒì?¯
š8enð„È³	¶�7€=%?¿&É)¹Üp´5æ‘Ñ³„d#íá¥6‚^-R§¶fz‘¡©W­ª–¥PnBoª†[¶U¿¥ÈJ¬¯xL´ÉFÕë¡êªã2[Ž:5uU}óŸL ¨¨ðmDç×º«Å	x:ÓDÍõgŠë’$òJCßzÔ^âMóçgìî9oŠ} ã�H1”².Ÿ�íG)ðeÓ…À²7tý:ÅÇç|^Ÿ�2˜EL÷:üPPÁÐ›ÂSÁt3q:ô W>	º™/‹¬÷î„Ë¨†;- æW=5Ì>ªp¯ƒêŽª;v+«ã)¿á® eòióê†|ìóO4Ä´„Ä    u   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  0+<?xml version="1.0"?><Features nextValidID="30"><Feature recentID="2" name="MCS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2818-2898" color="#99ccff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;multiple cloning site&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="29" name="AmpR" directionality="1" translationMW="31557.36" type="CDS" allowSegmentOverlaps="0" cleavageArrows="6846" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment name="signal sequence" range="6778-6846" color="#ccffcc" type="standard" translated="1"/><Segment range="6847-7638" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="28" name="EGFP" directionality="1" translationMW="26941.53" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="2935-2937" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="2938-2940" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="2941-3654" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="27" name="PuroR" directionality="1" translationMW="21496.48" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="4043-4642" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="26" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4709-5297" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="25" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7809-8397" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6192-6647" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3704-4033" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2234-2537" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="RRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1366-1599" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="3' LTR (Î”U3)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5384-5617" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;self-inactivating&amp;nbsp;3' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="RSV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="294-520" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Rous sarcoma virus enhancer/promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2538-2741" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="521-701" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3884-4019" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5850-5985" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="HIV-1 Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="748-873" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of human immunodeficiency virus type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5689-5810" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="cPPT/CTS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2095-2211" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;central polypurine tract and central termination sequence of HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="AmpR promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6673-6777" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="lac promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-35" range="149-154" color="#ffffff" type="standard"/><Segment range="155-172" color="#ffffff" type="standard"/><Segment name="-10" range="173-179" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="CAP binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="113-134" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T3 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="248-266" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T3 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="T7 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6006-6024" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="187-203" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="M13 rev" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="211-227" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6034-6050" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="Factor Xa site" directionality="2" translationMW="473.53" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5179" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="4796" maxFusedRunOn="4796" detectionMode="exactProteinMatch"><Segment range="5180-5191" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="1" name="linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3654" maxFusedRunOn="3654"><Segment range="2905-2919" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3654" maxFusedRunOn="3654"><Segment range="2920-2934" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>405fb08c-114c-411e-be3e-1395441a7d06</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:9:52">2018.9.13</Created>
<LastModified UTC="2:37:18">2025.6.3</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  <StrandColors><TopStrand><ColorRange range="0..2816" colors="black"/><ColorRange range="2898..8571" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..2816" colors="black"/><ColorRange range="2898..8571" colors="black"/></BottomStrand></StrandColors>  =_   
  {  !‚        !‚ÁDÏÖsWÏ»üÌ ñ­¥�87
¿L!3\Ö–!);{iÿC©Þ¦:ðì˜¢ Z!0eÖ­Ýtæa§=LÍi·•uS�8ÔébK¦5žè’è×`—JMv>�AU7ÈØñ �IB	ÆÈúitp.� q}áhóhÑ¢jR>NÆvWîà¤}„øîàåäýã¥>ÖTèú:Ô’"?.(ˆ{‚^Doíê’Ò U»“H3ñt¡S_D»±Í°èáLÍÃQ@0Ç‚sÕZ¡äÁED`#2ÒiL E¤lÆUk
Pô TUY•¥]™&S@NEÆ³J[àø¤Wm8‘ÕžÓ�}¿­ÑEéi™gI5þæˆ�µA‘TwÔêGQ‘UÕIGß7ML<áºÑøA„ZR™•™RIU¡}¤MýõQH#DEUH�N¢¾€®€M4Þ`3Lµ‡8p�GZh¸&HÓMZŽ9¡×M¸£^Ý. ÛSNô5ø8�QŸ•×Nø¨ŽàUí¨ßxâ>�c’�%•»�tj·qøÑ¥Ö�Ôég{éhQlÕ}5E…ÔZh‘•€Ô¨�h*]—Vƒˆ&eZke†I:	
”Y*£‰êæHY�Ð­ö›*ÑýûõÿñÐüPYQBDGDtokH\±Âl)z¥QT  	Ò4Qd‘Ùd×GgW•�UÕ§U­V©±”è¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M²¤…òÑ³àG=½Î*‡ïdQßä_äÎ|Ñ»µ:Å®4±Â|Àü÷Âì@Â4‚0“`„ÔA8­ð#öø,NrWu+L‹Ã1÷çNáJÛ�÷ÃS�Èþßì‡ßáùÃ#HëO9ÿvÓqë­KÏcñCœ½Íöë­AÇyÔ3Ä!JÄwø!|Û…lzÔp×o€7S�'^uÓÝræmƒÇSEµ^µÏuÛpÓ#NÏyôÑwÿl1ÃüŽ8üu÷Ÿ„ßÒóøMQÿ\E3vã�+!óÏ·»�Äâu%Ào’—2ÇÒâˆ‹J@ˆ/ô;ý44RcTØÛµ¤´×Ðˆ/ô;ý44RcTØÛµ¤´×Ù/Ïù{ÏÛóþ^óô¯?k¼ÿ`áåªªjcD<CÏ°û„æ½’Cª Cä:£UNŸañ'S÷Ï³ßÌqËýÉÍþÏ<ÊÏÏs=ß,oÏvÄÀ·ÄãQ{¯·3,Çl5€³3Ì Âà}ÏL±L Â+<FÃó6„Â¿3Mt`PûàÏ7ÃõüÈ+à÷Ë»?}P.LòÈïðHÃÄÌðüúøûÌÿ5ûþ¹ÄÃ°ë|·Ï±üP|Íø#aüÔü#áÏÏ÷Í3ññPLÜØÛ=,uµû�§U¨…F‡‚k¥æ(ïªs˜�œã©cê&ØæŽ¿&­ªï¾‰•¾
8»©„ˆ?(´5È2�â*8s_ýà £ß~-;êð¬êÿïæ�pÁ{lóãèüã�ÐÃŠxu¯ Š,N¾¯`ã³â(÷àkŒÀrúãœ¿¬?µ½0~¾¿0øãÃ¸Ãî¼Ë¬úÏíKïêÏ¿Ï�k°Ÿ©GÖ�CN$n“ÞªU@àP9k{õÅFãªŽ‰à.î
GÑ¸O€Nî^@_Þ:SíeN�¡#­I"#òâˆ‡¸%äFþÑþ©-"[¹4’JØ¶é¦´š™z5EafÑè©£N™`§VTÖM·V­Õ•6ª­ãk’ˆ*/W¶Ö&é¶.äæüÿ—¼ý¿?åï?Jóö»ÏöZª¦¦4CÄ<û¸NkÑI!:ª>@rZÏæHK&œÌíàòÊ§\²Wø2Ÿ×¥³èÓvÿêÏN–LQÍñ³úýt£4øX1`ÏâL3iLÃ ‚œÍ29Þ�LþLüï«:ºþ¾®Ïr³Ã«üµ;•°¿©¼jHêpß±ÿU^†�!‚·$€öÏá‘ÂªÏê KÜ®¥’º+Õà]wµþnÂæº¡©”¨ñ«š
UXN—Uj\Åª]Vis§Z�ƒz¬XŒÁæ¢¦ªågkVbo;aeþXëYf…TI„š×jò,þšþªŒ6¨úøªŽßÑ\à…ITc…J7�±xµÓ	Fú*³üQr«ÖMê•+ŽbÂi¿&‡<D×°³Í�æ»E‡ ¡'·ÒÝMºp`Øt”E¦4Ž=—ØHeÞ0õéë‡\l|PN_:ª7]€Ø—³èl Á8XSÙõqÄÈwÜcâM`×\£5y¥àÅç§¹:ü5Ö–G��R�Þ¸ÎÃúðàà©£†VàOL€»0›hÓxÑ‚Sûòl’›�Ç KC^`aqY8FB>ÞSh%âÑõ*{fg©•zÚ©jUä!Fú¨e»aõ[ú\„ªÊ÷„ËGü‘TmPnº¾ª®3%¸ã£WUWß9ôÂ
Š�ÔN}{ª¼P—ƒ­4LÑŸVx®¹"O$¤=÷­Eî$ß>v~Îã–ø§Ò8ôƒK"éð(Ôq’ŸPÍ0°8\ +#wOÓ L\~wÅáùÓ!9„TÏs¯Å	¼%0Ð,A7¯@sà›©’ø°*Ï~áèL±ªˆc²Ð Á>`UsÓ\Ãê§
ø>¨àú£·bº¾2›þâ_&Ÿ>¡!8gÎÏ4ñqñ3LKHLA    {   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  0+<?xml version="1.0"?><Features nextValidID="30"><Feature recentID="2" name="MCS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2824-2904" color="#99ccff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;multiple cloning site&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="29" name="AmpR" directionality="1" translationMW="31557.36" type="CDS" allowSegmentOverlaps="0" cleavageArrows="6852" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment name="signal sequence" range="6784-6852" color="#ccffcc" type="standard" translated="1"/><Segment range="6853-7644" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="28" name="EGFP" directionality="1" translationMW="26941.53" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="2941-2943" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="2944-2946" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="2947-3660" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="27" name="PuroR" directionality="1" translationMW="21496.48" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="4049-4648" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="26" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4715-5303" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="25" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7815-8403" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6198-6653" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3710-4039" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2234-2537" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="RRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1366-1599" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="3' LTR (Î”U3)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5390-5623" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;self-inactivating&amp;nbsp;3' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="RSV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="294-520" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Rous sarcoma virus enhancer/promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2538-2741" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="521-701" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3890-4025" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5856-5991" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="HIV-1 Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="748-873" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of human immunodeficiency virus type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5695-5816" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="cPPT/CTS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2095-2211" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;central polypurine tract and central termination sequence of HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="AmpR promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6679-6783" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="lac promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-35" range="149-154" color="#ffffff" type="standard"/><Segment range="155-172" color="#ffffff" type="standard"/><Segment name="-10" range="173-179" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="CAP binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="113-134" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T3 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="248-266" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T3 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="T7 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6012-6030" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="187-203" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="M13 rev" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="211-227" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6040-6056" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="Factor Xa site" directionality="2" translationMW="473.53" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5185" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="4802" maxFusedRunOn="4802" detectionMode="exactProteinMatch"><Segment range="5186-5197" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="1" name="linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3660" maxFusedRunOn="3660"><Segment range="2911-2925" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3660" maxFusedRunOn="3660"><Segment range="2926-2940" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ><Notes>
<UUID>405fb08c-114c-411e-be3e-1395441a7d06</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:9:52">2018.9.13</Created>
<LastModified UTC="2:37:9">2025.6.3</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  o<StrandColors><TopStrand><ColorRange range="0..2816" colors="black"/><ColorRange range="2817..2822" colors="light blue"/><ColorRange range="2904..8577" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..2816" colors="black"/><ColorRange range="2817..2822" colors="light blue"/><ColorRange range="2904..8577" colors="black"/></BottomStrand></StrandColors>  <=   	  g  !1        !1ÁDÏÖsWÏ»üÌ ñ­¥�87
¿L!3\Ö–!);{iÿC©Þ¦:ðì˜¢ Z!0eÖ­Ýtæa§=LÍi·•uS�8ÔébK¦5žè’è×`—JMv>�AU7ÈØñ �IB	ÆÈúitp.� q}áhóhÑ¢jR>NÆvWîà¤}„øîàåäýã¥>ÖTèú:Ô’"?.(ˆ{‚^Doíê’Ò U»“H3ñt¡S_D»±Í°èáLÍÃQ@0Ç‚sÕZ¡äÁED`#2ÒiL E¤lÆUk
Pô TUY•¥]™&S@NEÆ³J[àø¤Wm8‘ÕžÓ�}¿­ÑEéi™gI5þæˆ�µA‘TwÔêGQ‘UÕIGß7ML<áºÑøA„ZR™•™RIU¡}¤MýõQH#DEUH�N¢¾€®€M4Þ`3Lµ‡8p�GZh¸&HÓMZŽ9¡×M¸£^Ý. ÛSNô5ø8�QŸ•×Nø¨ŽàUí¨ßxâ>�c’�%•»�tj·qøÑ¥Ö�Ôég{éhQlÕ}5E…ÔZh‘•€Ô¨�h*]—Vƒˆ&eZke†I:	
”Y*£‰êæHY�Ð­ö›*ÑýûõÿñÐüPYQBDGDtokH\±Âl)z¥QT  	Ò4Qd‘Ùd×GgW•�UÕ§U­V©±”è¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M²¤…òÑ³àG=½Î*‡ïdQßä_±ÒÂM‚P1â·À �Ûà±81É\=Ô­2/@Äcßœ58…+n7ßN?#ø?²‡ç�#­<çü}ØSMÇ®µ/=�Årô7Û®µ5çQüÏ…+1ßà…ólN±ëPAÃ]¾ ÝN�y×OuË™¶M\6Ô!zÔo=×lAÃL�;<}çÓEßý°Çò8ãñ×Þ~KÏá5GýqÌmØ/Ž¬‡Ï<Þì6‰Ô—¼J\ËH,ˆ"-) T¿ÐïôÐÑI�ScnÖ’Ó_B T¿ÐïôÐÑI�ScnÖ’Ó_d¿?åï?oÏù{ÏÒ¼ý®óýƒ‡–ª©©�ñ>ÃîšôRHAª��ê�U:}‡Ä�OÜ#>Ï1Ç/ô'7û<ó+<yÿ=Ì÷|±¼=Ûß�Eî¾ÜÌ°;°Ô"ÌpÏ0ƒ�÷<2Å0¬ñÌØ<J
ü8<Í5Ñ�Cïƒ<ßÔó ¯ƒß,2ìü}ô@¸3Ë#¿Á C3À#óëàGï3ü×ïúçÃ­òß<rÄ#ñAó7à�‡óSð�‡??Ü4ÏÇÅA3qÿclô±Ö×î�V¢	®—˜£¾©ÎbsŽ¥�¨›cš:ü˜:¶«¾ú&Vø(âî¦J ü¢Ð× È"7ˆ¨áÍ÷‚€6�}ø´ï«Àz³«ÿ¿š=Ãí³Ï�£óŽ@;(áÖ¼‚(°8rú½ƒŽÏˆ£ß�®3ËëŽrþ°þÖôÁúúüÃã�ãºó.³ë?´q,¿¨>ÿ>¬NÂ~¥Xzu8‘ºOz©TU �@å­ï×uŽª:'€»¸)Fá>;¸9yxéOµ•:>„Žµ$ˆ�ËŠ"à—‘ûGú¤´ˆnäÒI+bÛ¦šÒjeèÕ…›DHD¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¦Ø¸;“›óþ^óöüÿ—¼ý+ÏÚï?Ø8yjªš˜Ñóì>á9¯E$„ê¨ùÉýk?™!,šs3·ƒË*�rÈx_àÊ^–Ï£MÛÿ«=82Y1D?7ÆÏëõÒŒÓá`Å€s?‰0Í¥3‚
s4È|çz=3ù3ó¾¬êëúú»=ÊÏ¯òÔîVÀþ¤
ñ©#©Ã~ÇýUz@†
Ü’Û?†G
«?¨r�/rº–Hè¯W�uÞ×ù»šê†¦P£Æ¬>h)Ua8j]U©s©uY¥Î�jBê°b3ÿšŠš«•�­Y‰¼í„u—øec­ešPQ&k]«È³úkúª0Ú£ëâª;Es‚%QŽm(ÜH
vÅàn×L%èDªÏñEÊ¯Xa7ªT®9ˆ3	¦üšð5^ÂÏ6wší‚„žßKtU6éÁƒaÒQ˜Ò8ö_a!—xÃ×§®q°AñA9|ê¨Ýv b^Ï¡²€_áaOgÕÇ!ßq�‰5ƒ]rŒÕæ—ƒžžäëð×ZY0eJ4zã;ëÃƒ‚¦Ž[�<!2ìÂm£MàF	OïÉ²Jnw-y�…ÅdtláûxiM —‹GÔ©í™ž¤hjUëj¥©T�…ê¡–í‡Õoér«+Þ-òEQµAºèFúª¸Ì–ãŽ�]U_|çÓ**<Q9õîªñB^´Ñ3F}Yâºä‰<’�÷Þµ¸“|ùÙû;Ž[âŸH8ãÒe,‹§À {QÆJ|C4ÂÀáp ¬�Ý?N�1qùß„gçL„æS=Î¿T0t&ð”Ã@°]LÜ@N½ Ï‚n¦KâÀ«=û‡¡2Æª!ŽË@ù�UÏMsªœ+àú£ƒêŽÝŠêøÊoøkˆ|š|ú„„áŸ;<ÓÅÇÄÍ1-!1     *   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  /<?xml version="1.0"?><Features recycledIDs="2" nextValidID="30"><Feature recentID="29" name="AmpR" directionality="1" translationMW="31557.36" type="CDS" allowSegmentOverlaps="0" cleavageArrows="6771" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment name="signal sequence" range="6703-6771" color="#ccffcc" type="standard" translated="1"/><Segment range="6772-7563" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="28" name="EGFP" directionality="1" translationMW="26941.53" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="2860-2862" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="2863-2865" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="2866-3579" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="27" name="PuroR" directionality="1" translationMW="21496.48" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3968-4567" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="26" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4634-5222" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="25" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7734-8322" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6117-6572" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3629-3958" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2234-2537" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="RRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1366-1599" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="3' LTR (Î”U3)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5309-5542" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;self-inactivating&amp;nbsp;3' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="RSV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="294-520" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Rous sarcoma virus enhancer/promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2538-2741" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="521-701" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3809-3944" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5775-5910" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="HIV-1 Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="748-873" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of human immunodeficiency virus type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5614-5735" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="cPPT/CTS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2095-2211" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;central polypurine tract and central termination sequence of HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="AmpR promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6598-6702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="lac promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-35" range="149-154" color="#ffffff" type="standard"/><Segment range="155-172" color="#ffffff" type="standard"/><Segment name="-10" range="173-179" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="CAP binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="113-134" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T3 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="248-266" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T3 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="T7 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5931-5949" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="187-203" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="M13 rev" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="211-227" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5959-5975" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="Factor Xa site" directionality="2" translationMW="473.53" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5104" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="4721" maxFusedRunOn="4721" detectionMode="exactProteinMatch"><Segment range="5105-5116" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="1" name="linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3579" maxFusedRunOn="3579"><Segment range="2830-2844" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3579" maxFusedRunOn="3579"><Segment range="2845-2859" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ><Notes>
<UUID>405fb08c-114c-411e-be3e-1395441a7d06</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:9:52">2018.9.13</Created>
<LastModified UTC="2:37:3">2025.6.3</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  o<StrandColors><TopStrand><ColorRange range="0..2816" colors="black"/><ColorRange range="2817..2822" colors="light blue"/><ColorRange range="2823..8496" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..2816" colors="black"/><ColorRange range="2817..2822" colors="light blue"/><ColorRange range="2823..8496" colors="black"/></BottomStrand></StrandColors>  <?     h  !7        !7ÁDÏÖsWÏ»üÌ ñ­¥�87
¿L!3\Ö–!);{iÿC©Þ¦:ðì˜¢ Z!0eÖ­Ýtæa§=LÍi·•uS�8ÔébK¦5žè’è×`—JMv>�AU7ÈØñ �IB	ÆÈúitp.� q}áhóhÑ¢jR>NÆvWîà¤}„øîàåäýã¥>ÖTèú:Ô’"?.(ˆ{‚^Doíê’Ò U»“H3ñt¡S_D»±Í°èáLÍÃQ@0Ç‚sÕZ¡äÁED`#2ÒiL E¤lÆUk
Pô TUY•¥]™&S@NEÆ³J[àø¤Wm8‘ÕžÓ�}¿­ÑEéi™gI5þæˆ�µA‘TwÔêGQ‘UÕIGß7ML<áºÑøA„ZR™•™RIU¡}¤MýõQH#DEUH�N¢¾€®€M4Þ`3Lµ‡8p�GZh¸&HÓMZŽ9¡×M¸£^Ý. ÛSNô5ø8�QŸ•×Nø¨ŽàUí¨ßxâ>�c’�%•»�tj·qøÑ¥Ö�Ôég{éhQlÕ}5E…ÔZh‘•€Ô¨�h*]—Vƒˆ&eZke†I:	
”Y*£‰êæHY�Ð­ö›*ÑýûõÿñÐüPYQBDGDtokH\±Âl)z¥QT  	Ò4Qd‘Ùd×GgW•�UÕ§U­V©±”è¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M²¤…òÑ³àG=½Î*‡ïdQßä_	N»0� �$Ø!5N+|ý¾�Ã•ÃÝJÓ"ðÄ@Æ=ùÃS‡øR¶ã}ðÔãò?ƒ÷û!÷ø~pÈÒ:ÓÎÇÝ…4ÜzëRóØüPç/A3}ºëPqÃ^uÌñR±þ_6Äá@Ûµ5ÛàÔàI×�t÷\¹›`ñÔÑEÃmB­FóÝvÄ4ÈÓ³ÇÞ}4]ÿÛpÿ#Ž?}çá7ô¼þT×LÆÝ‚øàJÈ|óÀmîÃ`q8�IpÁä¥Ì±ô‚Çø�b"Ò�"KýÿM˜Õ66íi-5ô"KýÿM˜Õ66íi-5öKóþ^óöüÿ—¼ý+ÏÚï?Ø8yjªš˜Ñóì>á9¯E$„ê¨ù¨ÕS§Ø|IÔýÂ3ì÷órÿ@òs³Ï2³ÇŸóÜÏwËÁóÝ±0-ñ8Ô^ëíÌË±ÛB ,ÁÇó0¸sÁ,S 0ŠÏ°üÍƒÄ¡0¯ÃƒÌÓ]>ø3ÍðýA?2
ø=òÃ.ÏÇß@Ô€Ó<²;ü0ñ3<?>¾~ó?Í~ÿ®q0ì:ß-óÇ,B?3~Ø5?øsóýÇóLü|T7ö6ÏKm~àiÕj!Q¡àšéyŠ;êœæ g8êXú‰¶9£¯Éƒ«j»ï¢eo‚Ž.êa¢Ê-r‚#xŠŽ×ÿx(h÷ß‹Nú¼«:¿ûù£Ü0^Û<øú?8àt°â€ÞkÈ"‹ S‡/«Ø8ìøŠ=øã0¾¸ç/ëíoL¯¯Ì>8ðî0û¯2ë>³ûGÁ»ú€óïóàZÄì'êQõ‡§PÓ‰¤÷ª•EP xZÞýqGQ¸ê£¢x»‚‘ônà»ƒ—�÷Ž”ûYS£èHëRHˆü¸¢!î	y¿´ªKH�VîM$’¶-ºi­&¦^�QXY´D„Az*hÓ¦X)Õ•5“mÕ«ueMª«xÚä¢
‹Õíµ‰ºm‹ƒ¹9¿?åï?oÏù{ÏÒ¼ý®óýƒ‡–ª©©�ñ>ÃîšôRHAª��ŸÖ³ù’É§3;x<²©×,‡€Uþ§õélú4Ý¿ú³Óƒ%“Có|lþ¿](Í>X3ø“ÚS0Àˆ §3L‡Îw£Ó?“?;êÎ®¿¯«³Ü¬ðêÿ-Nål ïê@¯’:œ7ìÕW¡¤`­É =³ødpª³ú‡(÷+©d�îŠõx]í›°¹®¨jeª<jÃæ‚•V†¥ÕZ—1j—Uš\éÖ¤ Þ« V#0_ù¨©ª¹YÚÕ˜›ÎØGY†V:ÖY¡Ua&µÚ¼‹?¦¿ª£ª>¾*£·ôW8!RUáFÒ€�Ä€§l^ítÂQ¾„J¬ÿ\ªõ†z¥Jã˜ƒ0šoÉ¡ÏQ5ì,ógy®Ñaè(Iíô·ESnœ6%i�#�eöwŒ=zzá×—Îª�×`¶%ìú(ðNö}\q2÷ø“X5×(Í^ix1yéîN¿u¥‘à`CT£A÷®3°þ¼88*há•¸Â .Ì&Ú4Þ ô`”þü›$¦çqÀÐ×˜\VGFÎ��·†”Ú	x´}JžÙ™êF†¥^¶ªZ•A¹Q¾ªnØ}Vþ—!*²½á2Ñÿ$UT®„oª«ŒÉn8èÕÕU÷Î}0‚¢£ÁµŸ^ê¯%àëM4gÕž+®H“É)}ëQ{‰7Ï�Ÿ³¸å¾)ôƒŽ= ÆRÈº|
µd§Á”3L, 
ÈÝÓôè�ñxF~tÈNa3Üëñ@ECBo	L4ÐDÍÄëÐ\ø&êd¾,
³ß¸z,j¢ì´ 0O˜\ô×0ú©Â¾ª8>¨íØ®¯Œ¦ÿ†¸�—É§Ï¨HNó³Í<\|LÓÒ    0   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  /<?xml version="1.0"?><Features recycledIDs="2" nextValidID="30"><Feature recentID="29" name="AmpR" directionality="1" translationMW="31557.36" type="CDS" allowSegmentOverlaps="0" cleavageArrows="6777" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment name="signal sequence" range="6709-6777" color="#ccffcc" type="standard" translated="1"/><Segment range="6778-7569" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="28" name="EGFP" directionality="1" translationMW="26941.53" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="2866-2868" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="2869-2871" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="2872-3585" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="27" name="PuroR" directionality="1" translationMW="21496.48" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3974-4573" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="26" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4640-5228" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="25" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7740-8328" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6123-6578" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3635-3964" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2234-2537" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="RRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1366-1599" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="3' LTR (Î”U3)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5315-5548" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;self-inactivating&amp;nbsp;3' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="RSV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="294-520" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Rous sarcoma virus enhancer/promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2538-2741" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="521-701" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3815-3950" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5781-5916" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="HIV-1 Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="748-873" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of human immunodeficiency virus type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5620-5741" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="cPPT/CTS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2095-2211" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;central polypurine tract and central termination sequence of HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="AmpR promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6604-6708" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="lac promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-35" range="149-154" color="#ffffff" type="standard"/><Segment range="155-172" color="#ffffff" type="standard"/><Segment name="-10" range="173-179" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="CAP binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="113-134" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T3 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="248-266" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T3 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="T7 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5937-5955" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="187-203" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="M13 rev" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="211-227" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5965-5981" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="Factor Xa site" directionality="2" translationMW="473.53" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5110" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="4727" maxFusedRunOn="4727" detectionMode="exactProteinMatch"><Segment range="5111-5122" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="1" name="linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3585" maxFusedRunOn="3585"><Segment range="2836-2850" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3585" maxFusedRunOn="3585"><Segment range="2851-2865" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>405fb08c-114c-411e-be3e-1395441a7d06</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:9:52">2018.9.13</Created>
<LastModified UTC="2:36:28">2025.6.3</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  o<StrandColors><TopStrand><ColorRange range="0..2816" colors="black"/><ColorRange range="2817..2822" colors="light blue"/><ColorRange range="2829..8502" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..2816" colors="black"/><ColorRange range="2817..2822" colors="light blue"/><ColorRange range="2829..8502" colors="black"/></BottomStrand></StrandColors>  <>     g  !1        !1ÁDÏÖsWÏ»üÌ ñ­¥�87
¿L!3\Ö–!);{iÿC©Þ¦:ðì˜¢ Z!0eÖ­Ýtæa§=LÍi·•uS�8ÔébK¦5žè’è×`—JMv>�AU7ÈØñ �IB	ÆÈúitp.� q}áhóhÑ¢jR>NÆvWîà¤}„øîàåäýã¥>ÖTèú:Ô’"?.(ˆ{‚^Doíê’Ò U»“H3ñt¡S_D»±Í°èáLÍÃQ@0Ç‚sÕZ¡äÁED`#2ÒiL E¤lÆUk
Pô TUY•¥]™&S@NEÆ³J[àø¤Wm8‘ÕžÓ�}¿­ÑEéi™gI5þæˆ�µA‘TwÔêGQ‘UÕIGß7ML<áºÑøA„ZR™•™RIU¡}¤MýõQH#DEUH�N¢¾€®€M4Þ`3Lµ‡8p�GZh¸&HÓMZŽ9¡×M¸£^Ý. ÛSNô5ø8�QŸ•×Nø¨ŽàUí¨ßxâ>�c’�%•»�tj·qøÑ¥Ö�Ôég{éhQlÕ}5E…ÔZh‘•€Ô¨�h*]—Vƒˆ&eZke†I:	
”Y*£‰êæHY�Ð­ö›*ÑýûõÿñÐüPYQBDGDtokH\±Âl)z¥QT  	Ò4Qd‘Ùd×GgW•�UÕ§U­V©±”è¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M²¤…òÑ³àG=½Î*‡ïdQßä_±ÒÂM‚P1â·À �Ûà±81É\=Ô­2/@Äcßœ58…+n7ßN?#ø?²‡ç�#­<çü}ØSMÇ®µ/=�Årô7Û®µ5çQüÏ…+1ßà…ólN±ëPAÃ]¾ ÝN�y×OuË™¶M\6Ô!zÔo=×lAÃL�;<}çÓEßý°Çò8ãñ×Þ~KÏá5GýqÌmØ/Ž¬‡Ï<Þì6‰Ô—¼J\ËH,ˆ"-) T¿ÐïôÐÑI�ScnÖ’Ó_B T¿ÐïôÐÑI�ScnÖ’Ó_d¿?åï?oÏù{ÏÒ¼ý®óýƒ‡–ª©©�ñ>ÃîšôRHAª��ê�U:}‡Ä�OÜ#>Ï1Ç/ô'7û<ó+<yÿ=Ì÷|±¼=Ûß�Eî¾ÜÌ°;°Ô"ÌpÏ0ƒ�÷<2Å0¬ñÌØ<J
ü8<Í5Ñ�Cïƒ<ßÔó ¯ƒß,2ìü}ô@¸3Ë#¿Á C3À#óëàGï3ü×ïúçÃ­òß<rÄ#ñAó7à�‡óSð�‡??Ü4ÏÇÅA3qÿclô±Ö×î�V¢	®—˜£¾©ÎbsŽ¥�¨›cš:ü˜:¶«¾ú&Vø(âî¦J ü¢Ð× È"7ˆ¨áÍ÷‚€6�}ø´ï«Àz³«ÿ¿š=Ãí³Ï�£óŽ@;(áÖ¼‚(°8rú½ƒŽÏˆ£ß�®3ËëŽrþ°þÖôÁúúüÃã�ãºó.³ë?´q,¿¨>ÿ>¬NÂ~¥Xzu8‘ºOz©TU �@å­ï×uŽª:'€»¸)Fá>;¸9yxéOµ•:>„Žµ$ˆ�ËŠ"à—‘ûGú¤´ˆnäÒI+bÛ¦šÒjeèÕ…›DHD¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¦Ø¸;“›óþ^óöüÿ—¼ý+ÏÚï?Ø8yjªš˜Ñóì>á9¯E$„ê¨ùÉýk?™!,šs3·ƒË*�rÈx_àÊ^–Ï£MÛÿ«=82Y1D?7ÆÏëõÒŒÓá`Å€s?‰0Í¥3‚
s4È|çz=3ù3ó¾¬êëúú»=ÊÏ¯òÔîVÀþ¤
ñ©#©Ã~ÇýUz@†
Ü’Û?†G
«?¨r�/rº–Hè¯W�uÞ×ù»šê†¦P£Æ¬>h)Ua8j]U©s©uY¥Î�jBê°b3ÿšŠš«•�­Y‰¼í„u—øec­ešPQ&k]«È³úkúª0Ú£ëâª;Es‚%QŽm(ÜH
vÅàn×L%èDªÏñEÊ¯Xa7ªT®9ˆ3	¦üšð5^ÂÏ6wší‚„žßKtU6éÁƒaÒQ˜Ò8ö_a!—xÃ×§®q°AñA9|ê¨Ýv b^Ï¡²€_áaOgÕÇ!ßq�‰5ƒ]rŒÕæ—ƒžžäëð×ZY0eJ4zã;ëÃƒ‚¦Ž[�<!2ìÂm£MàF	OïÉ²Jnw-y�…ÅdtláûxiM —‹GÔ©í™ž¤hjUëj¥©T�…ê¡–í‡Õoér«+Þ-òEQµAºèFúª¸Ì–ãŽ�]U_|çÓ**<Q9õîªñB^´Ñ3F}Yâºä‰<’�÷Þµ¸“|ùÙû;Ž[âŸH8ãÒe,‹§À {QÆJ|C4ÂÀáp ¬�Ý?N�1qùß„gçL„æS=Î¿T0t&ð”Ã@°]LÜ@N½ Ï‚n¦KâÀ«=û‡¡2Æª!ŽË@ù�UÏMsªœ+àú£ƒêŽÝŠêøÊoøkˆ|š|ú„„áŸ;<ÓÅÇÄÍ1-!1     *   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  /<?xml version="1.0"?><Features recycledIDs="2" nextValidID="30"><Feature recentID="29" name="AmpR" directionality="1" translationMW="31557.36" type="CDS" allowSegmentOverlaps="0" cleavageArrows="6771" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment name="signal sequence" range="6703-6771" color="#ccffcc" type="standard" translated="1"/><Segment range="6772-7563" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="28" name="EGFP" directionality="1" translationMW="26941.53" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="2860-2862" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="2863-2865" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="2866-3579" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="27" name="PuroR" directionality="1" translationMW="21496.48" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3968-4567" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="26" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4634-5222" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="25" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7734-8322" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6117-6572" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3629-3958" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2234-2537" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="RRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1366-1599" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="3' LTR (Î”U3)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5309-5542" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;self-inactivating&amp;nbsp;3' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="RSV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="294-520" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Rous sarcoma virus enhancer/promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2538-2741" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="521-701" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3809-3944" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5775-5910" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="HIV-1 Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="748-873" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of human immunodeficiency virus type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5614-5735" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="cPPT/CTS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2095-2211" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;central polypurine tract and central termination sequence of HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="AmpR promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6598-6702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="lac promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-35" range="149-154" color="#ffffff" type="standard"/><Segment range="155-172" color="#ffffff" type="standard"/><Segment name="-10" range="173-179" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="CAP binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="113-134" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T3 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="248-266" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T3 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="T7 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5931-5949" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="187-203" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="M13 rev" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="211-227" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5959-5975" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="Factor Xa site" directionality="2" translationMW="473.53" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5104" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="4721" maxFusedRunOn="4721" detectionMode="exactProteinMatch"><Segment range="5105-5116" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="1" name="linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3579" maxFusedRunOn="3579"><Segment range="2830-2844" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3579" maxFusedRunOn="3579"><Segment range="2845-2859" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>405fb08c-114c-411e-be3e-1395441a7d06</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:9:52">2018.9.13</Created>
<LastModified UTC="2:36:22">2025.6.3</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  o<StrandColors><TopStrand><ColorRange range="0..2816" colors="black"/><ColorRange range="2817..2822" colors="light blue"/><ColorRange range="2823..8496" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..2816" colors="black"/><ColorRange range="2817..2822" colors="light blue"/><ColorRange range="2823..8496" colors="black"/></BottomStrand></StrandColors>  ;{     e  !+        !+ÁDÏÖsWÏ»üÌ ñ­¥�87
¿L!3\Ö–!);{iÿC©Þ¦:ðì˜¢ Z!0eÖ­Ýtæa§=LÍi·•uS�8ÔébK¦5žè’è×`—JMv>�AU7ÈØñ �IB	ÆÈúitp.� q}áhóhÑ¢jR>NÆvWîà¤}„øîàåäýã¥>ÖTèú:Ô’"?.(ˆ{‚^Doíê’Ò U»“H3ñt¡S_D»±Í°èáLÍÃQ@0Ç‚sÕZ¡äÁED`#2ÒiL E¤lÆUk
Pô TUY•¥]™&S@NEÆ³J[àø¤Wm8‘ÕžÓ�}¿­ÑEéi™gI5þæˆ�µA‘TwÔêGQ‘UÕIGß7ML<áºÑøA„ZR™•™RIU¡}¤MýõQH#DEUH�N¢¾€®€M4Þ`3Lµ‡8p�GZh¸&HÓMZŽ9¡×M¸£^Ý. ÛSNô5ø8�QŸ•×Nø¨ŽàUí¨ßxâ>�c’�%•»�tj·qøÑ¥Ö�Ôég{éhQlÕ}5E…ÔZh‘•€Ô¨�h*]—Vƒˆ&eZke†I:	
”Y*£‰êæHY�Ð­ö›*ÑýûõÿñÐüPYQBDGDtokH\±Âl)z¥QT  	Ò4Qd‘Ùd×GgW•�UÕ§U­V©±”è¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M²¤…òÑ³àG=½Î*‡ïdQßä{0� �$Ø!5N+|ý¾�Ã•ÃÝJÓ"ðÄ@Æ=ùÃS‡øR¶ã}ðÔãò?ƒ÷û!÷ø~pÈÒ:ÓÎÇÝ…4ÜzëRóØüPç/A3}ºëPqÃ^uÌñR±þ_6Äá@Ûµ5ÛàÔàI×�t÷\¹›`ñÔÑEÃmB­FóÝvÄ4ÈÓ³ÇÞ}4]ÿÛpÿ#Ž?}çá7ô¼þT×LÆÝ‚øàJÈ|óÀmîÃ`q8�IpÁä¥Ì±ô‚Çø�b"Ò�"KýÿM˜Õ66íi-5ô"KýÿM˜Õ66íi-5öKóþ^óöüÿ—¼ý+ÏÚï?Ø8yjªš˜Ñóì>á9¯E$„ê¨ù¨ÕS§Ø|IÔýÂ3ì÷órÿ@òs³Ï2³ÇŸóÜÏwËÁóÝ±0-ñ8Ô^ëíÌË±ÛB ,ÁÇó0¸sÁ,S 0ŠÏ°üÍƒÄ¡0¯ÃƒÌÓ]>ø3ÍðýA?2
ø=òÃ.ÏÇß@Ô€Ó<²;ü0ñ3<?>¾~ó?Í~ÿ®q0ì:ß-óÇ,B?3~Ø5?øsóýÇóLü|T7ö6ÏKm~àiÕj!Q¡àšéyŠ;êœæ g8êXú‰¶9£¯Éƒ«j»ï¢eo‚Ž.êa¢Ê-r‚#xŠŽ×ÿx(h÷ß‹Nú¼«:¿ûù£Ü0^Û<øú?8àt°â€ÞkÈ"‹ S‡/«Ø8ìøŠ=øã0¾¸ç/ëíoL¯¯Ì>8ðî0û¯2ë>³ûGÁ»ú€óïóàZÄì'êQõ‡§PÓ‰¤÷ª•EP xZÞýqGQ¸ê£¢x»‚‘ônà»ƒ—�÷Ž”ûYS£èHëRHˆü¸¢!î	y¿´ªKH�VîM$’¶-ºi­&¦^�QXY´D„Az*hÓ¦X)Õ•5“mÕ«ueMª«xÚä¢
‹Õíµ‰ºm‹ƒ¹9¿?åï?oÏù{ÏÒ¼ý®óýƒ‡–ª©©�ñ>ÃîšôRHAª��ŸÖ³ù’É§3;x<²©×,‡€Uþ§õélú4Ý¿ú³Óƒ%“Có|lþ¿](Í>X3ø“ÚS0Àˆ §3L‡Îw£Ó?“?;êÎ®¿¯«³Ü¬ðêÿ-Nål ïê@¯’:œ7ìÕW¡¤`­É =³ødpª³ú‡(÷+©d�îŠõx]í›°¹®¨jeª<jÃæ‚•V†¥ÕZ—1j—Uš\éÖ¤ Þ« V#0_ù¨©ª¹YÚÕ˜›ÎØGY†V:ÖY¡Ua&µÚ¼‹?¦¿ª£ª>¾*£·ôW8!RUáFÒ€�Ä€§l^ítÂQ¾„J¬ÿ\ªõ†z¥Jã˜ƒ0šoÉ¡ÏQ5ì,ógy®Ñaè(Iíô·ESnœ6%i�#�eöwŒ=zzá×—Îª�×`¶%ìú(ðNö}\q2÷ø“X5×(Í^ix1yéîN¿u¥‘à`CT£A÷®3°þ¼88*há•¸Â .Ì&Ú4Þ ô`”þü›$¦çqÀÐ×˜\VGFÎ��·†”Ú	x´}JžÙ™êF†¥^¶ªZ•A¹Q¾ªnØ}Vþ—!*²½á2Ñÿ$UT®„oª«ŒÉn8èÕÕU÷Î}0‚¢£ÁµŸ^ê¯%àëM4gÕž+®H“É)}ëQ{‰7Ï�Ÿ³¸å¾)ôƒŽ= ÆRÈº|
µd§Á”3L, 
ÈÝÓôè�ñxF~tÈNa3Üëñ@ECBo	L4ÐDÍÄëÐ\ø&êd¾,
³ß¸z,j¢ì´ 0O˜\ô×0ú©Â¾ª8>¨íØ®¯Œ¦ÿ†¸�—É§Ï¨HNó³Í<\|LÓÒ    $   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  /<?xml version="1.0"?><Features recycledIDs="2" nextValidID="30"><Feature recentID="29" name="AmpR" directionality="1" translationMW="31557.36" type="CDS" allowSegmentOverlaps="0" cleavageArrows="6765" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment name="signal sequence" range="6697-6765" color="#ccffcc" type="standard" translated="1"/><Segment range="6766-7557" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="28" name="EGFP" directionality="1" translationMW="26941.53" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="2854-2856" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="2857-2859" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="2860-3573" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="27" name="PuroR" directionality="1" translationMW="21496.48" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3962-4561" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="26" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4628-5216" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="25" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7728-8316" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6111-6566" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3623-3952" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2234-2537" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="RRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1366-1599" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="3' LTR (Î”U3)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5303-5536" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;self-inactivating&amp;nbsp;3' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="RSV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="294-520" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Rous sarcoma virus enhancer/promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2538-2741" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="521-701" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3803-3938" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5769-5904" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="HIV-1 Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="748-873" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of human immunodeficiency virus type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5608-5729" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="cPPT/CTS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2095-2211" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;central polypurine tract and central termination sequence of HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="AmpR promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6592-6696" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="lac promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-35" range="149-154" color="#ffffff" type="standard"/><Segment range="155-172" color="#ffffff" type="standard"/><Segment name="-10" range="173-179" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="CAP binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="113-134" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T3 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="248-266" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T3 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="T7 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5925-5943" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="187-203" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="M13 rev" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="211-227" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5953-5969" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="Factor Xa site" directionality="2" translationMW="473.53" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5098" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="4715" maxFusedRunOn="4715" detectionMode="exactProteinMatch"><Segment range="5099-5110" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="1" name="linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3573" maxFusedRunOn="3573"><Segment range="2824-2838" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3573" maxFusedRunOn="3573"><Segment range="2839-2853" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ><Notes>
<UUID>405fb08c-114c-411e-be3e-1395441a7d06</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:9:52">2018.9.13</Created>
<LastModified UTC="2:36:2">2025.6.3</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   ¯<StrandColors><TopStrand><ColorRange range="2823..2852" colors="black"/></TopStrand><BottomStrand><ColorRange range="2823..2852" colors="black"/></BottomStrand></StrandColors>  =u     	  #×        #×ÁDÏÖsWÏ»üÌ ñ­¥�87
¿L!3\Ö–!);{iÿC©Þ¦:ðì˜¢ Z!0eÖ­Ýtæa§=LÍi·•uS�8ÔébK¦5žè’è×`—JMv>�AU7ÈØñ �IB	ÆÈúitp.� q}áhóhÑ¢jR>NÆvWîà¤}„øîàåäýã¥>ÖTèú:Ô’"?.(ˆ{‚^Doíê’Ò U»“H3ñt¡S_D»±Í°èáLÍÃQ@0Ç‚sÕZ¡äÁED`#2ÒiL E¤lÆUk
Pô TUY•¥]™&S@NEÆ³J[àø¤Wm8‘ÕžÓ�}¿­ÑEéi™gI5þæˆ�µA‘TwÔêGQ‘UÕIGß7ML<áºÑøA„ZR™•™RIU¡}¤MýõQH#DEUH�N¢¾€®€M4Þ`3Lµ‡8p�GZh¸&HÓMZŽ9¡×M¸£^Ý. ÛSNô5ø8�QŸ•×Nø¨ŽàUí¨ßxâ>�c’�%•»�tj·qøÑ¥Ö�Ôég{éhQlÕ}5E…ÔZh‘•€Ô¨�h*]—Vƒˆ&eZke†I:	
”Y*£‰êæHY�Ð­ö›*ÑýûõÿñÐüPYQBDGDtokH\±Âl)z¥QT  	Ò4Qd‘Ùd×GgW•�UÕ§U­V©±”è¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M²¤…òÑ³àG=½Î*‡ïdQßäO}�T“R6ÕŸŽë¥ën¸;86�#£š˜;Æ±îÑõn±5eö½*çt'k†�<ûaŽ

¾Ž N'Q/Ò68Šë¾è!šÏhFO<òàg÷PaiQ{ÐJ§PwçÕJpMA…ÿLwµOof xâ“‚ú�4©»~?ÔWN¨*ô)OãøSÛHBë]u+Ý¶�4€›³È!†ë†ëM†øŽ3ÛÍñ‚û0� �$Ø!5N+|ý¾�Ã•ÃÝJÓ"ðÄ@Æ=ùÃS‡øR¶ã}ðÔãò?ƒ÷û!÷ø~pÈÒ:ÓÎÇÝ…4ÜzëRóØüPç/A3}ºëPqÃ^uÌñR±þ_6Äá@Ûµ5ÛàÔàI×�t÷\¹›`ñÔÑEÃmB­FóÝvÄ4ÈÓ³ÇÞ}4]ÿÛpÿ#Ž?}çá7ô¼þT×LÆÝ‚øàJÈ|óÀmîÃ`q8�IpÁä¥Ì±ô‚Çø�b"Ò�"KýÿM˜Õ66íi-5ô"KýÿM˜Õ66íi-5öKóþ^óöüÿ—¼ý+ÏÚï?Ø8yjªš˜Ñóì>á9¯E$„ê¨ù¨ÕS§Ø|IÔýÂ3ì÷órÿ@òs³Ï2³ÇŸóÜÏwËÁóÝ±0-ñ8Ô^ëíÌË±ÛB ,ÁÇó0¸sÁ,S 0ŠÏ°üÍƒÄ¡0¯ÃƒÌÓ]>ø3ÍðýA?2
ø=òÃ.ÏÇß@Ô€Ó<²;ü0ñ3<?>¾~ó?Í~ÿ®q0ì:ß-óÇ,B?3~Ø5?øsóýÇóLü|T7ö6ÏKm~àiÕj!Q¡àšéyŠ;êœæ g8êXú‰¶9£¯Éƒ«j»ï¢eo‚Ž.êa¢Ê-r‚#xŠŽ×ÿx(h÷ß‹Nú¼«:¿ûù£Ü0^Û<øú?8àt°â€ÞkÈ"‹ S‡/«Ø8ìøŠ=øã0¾¸ç/ëíoL¯¯Ì>8ðî0û¯2ë>³ûGÁ»ú€óïóàZÄì'êQõ‡§PÓ‰¤÷ª•EP xZÞýqGQ¸ê£¢x»‚‘ônà»ƒ—�÷Ž”ûYS£èHëRHˆü¸¢!î	y¿´ªKH�VîM$’¶-ºi­&¦^�QXY´D„Az*hÓ¦X)Õ•5“mÕ«ueMª«xÚä¢
‹Õíµ‰ºm‹ƒ¹9¿?åï?oÏù{ÏÒ¼ý®óýƒ‡–ª©©�ñ>ÃîšôRHAª��ŸÖ³ù’É§3;x<²©×,‡€Uþ§õélú4Ý¿ú³Óƒ%“Có|lþ¿](Í>X3ø“ÚS0Àˆ §3L‡Îw£Ó?“?;êÎ®¿¯«³Ü¬ðêÿ-Nål ïê@¯’:œ7ìÕW¡¤`­É =³ødpª³ú‡(÷+©d�îŠõx]í›°¹®¨jeª<jÃæ‚•V†¥ÕZ—1j—Uš\éÖ¤ Þ« V#0_ù¨©ª¹YÚÕ˜›ÎØGY†V:ÖY¡Ua&µÚ¼‹?¦¿ª£ª>¾*£·ôW8!RUáFÒ€�Ä€§l^ítÂQ¾„J¬ÿ\ªõ†z¥Jã˜ƒ0šoÉ¡ÏQ5ì,ógy®Ñaè(Iíô·ESnœ6%i�#�eöwŒ=zzá×—Îª�×`¶%ìú(ðNö}\q2÷ø“X5×(Í^ix1yéîN¿u¥‘à`CT£A÷®3°þ¼88*há•¸Â .Ì&Ú4Þ ô`”þü›$¦çqÀÐ×˜\VGFÎ��·†”Ú	x´}JžÙ™êF†¥^¶ªZ•A¹Q¾ªnØ}Vþ—!*²½á2Ñÿ$UT®„oª«ŒÉn8èÕÕU÷Î}0‚¢£ÁµŸ^ê¯%àëM4gÕž+®H“É)}ëQ{‰7Ï�Ÿ³¸å¾)ôƒŽ= ÆRÈº|
µd§Á”3L, 
ÈÝÓôè�ñxF~tÈNa3Üëñ@ECBo	L4ÐDÍÄëÐ\ø&êd¾,
³ß¸z,j¢ì´ 0O˜\ô×0ú©Â¾ª8>¨íØ®¯Œ¦ÿ†¸�—É§Ï¨HNó³Í<\|LÓÒ    Ð   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  0k<?xml version="1.0"?><Features nextValidID="30"><Feature recentID="29" name="AmpR" directionality="1" translationMW="31558.16" type="CDS" allowSegmentOverlaps="0" cleavageArrows="7449" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment name="signal sequence" range="7381-7449" color="#ccffcc" type="standard" translated="1"/><Segment range="7450-8241" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="28" name="EGFP" directionality="1" translationMW="26942.08" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="3538-3540" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="3541-3543" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="3544-4257" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="27" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="4646-5245" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="26" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5312-5900" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="25" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8412-9000" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6795-7250" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4307-4636" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2234-2537" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="RRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1366-1599" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="3' LTR (Î”U3)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5987-6220" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;self-inactivating&amp;nbsp;3' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="RSV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="294-520" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Rous sarcoma virus enhancer/promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2538-2741" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="521-701" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4487-4622" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6453-6588" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="HIV-1 Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="748-873" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of human immunodeficiency virus type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6292-6413" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="cPPT/CTS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2095-2211" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;central polypurine tract and central termination sequence of HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="AmpR promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7276-7380" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="lac promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-35" range="149-154" color="#ffffff" type="standard"/><Segment range="155-172" color="#ffffff" type="standard"/><Segment name="-10" range="173-179" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="CAP binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="113-134" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T3 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="248-266" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T3 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="T7 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6609-6627" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="187-203" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="M13 rev" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="211-227" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6637-6653" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="Factor Xa site" directionality="2" translationMW="473.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5782" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="5399" maxFusedRunOn="5399" detectionMode="exactProteinMatch"><Segment range="5783-5794" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="2" name="Cd9-m" directionality="1" translationMW="25258.64" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4257" maxFusedRunOn="4257"><Segment range="2824-3501" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="1" name="linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4257" maxFusedRunOn="4257"><Segment range="3508-3522" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4257" maxFusedRunOn="4257"><Segment range="3523-3537" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>405fb08c-114c-411e-be3e-1395441a7d06</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:9:52">2018.9.13</Created>
<LastModified UTC="2:35:36">2025.6.3</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   ¯<StrandColors><TopStrand><ColorRange range="3507..3536" colors="black"/></TopStrand><BottomStrand><ColorRange range="3507..3536" colors="black"/></BottomStrand></StrandColors>  >     	2  $=        $=ÁDÏÖsWÏ»üÌ ñ­¥�87
¿L!3\Ö–!);{iÿC©Þ¦:ðì˜¢ Z!0eÖ­Ýtæa§=LÍi·•uS�8ÔébK¦5žè’è×`—JMv>�AU7ÈØñ �IB	ÆÈúitp.� q}áhóhÑ¢jR>NÆvWîà¤}„øîàåäýã¥>ÖTèú:Ô’"?.(ˆ{‚^Doíê’Ò U»“H3ñt¡S_D»±Í°èáLÍÃQ@0Ç‚sÕZ¡äÁED`#2ÒiL E¤lÆUk
Pô TUY•¥]™&S@NEÆ³J[àø¤Wm8‘ÕžÓ�}¿­ÑEéi™gI5þæˆ�µA‘TwÔêGQ‘UÕIGß7ML<áºÑøA„ZR™•™RIU¡}¤MýõQH#DEUH�N¢¾€®€M4Þ`3Lµ‡8p�GZh¸&HÓMZŽ9¡×M¸£^Ý. ÛSNô5ø8�QŸ•×Nø¨ŽàUí¨ßxâ>�c’�%•»�tj·qøÑ¥Ö�Ôég{éhQlÕ}5E…ÔZh‘•€Ô¨�h*]—Vƒˆ&eZke†I:	
”Y*£‰êæHY�Ð­ö›*ÑýûõÿñÐüPYQBDGDtokH\±Âl)z¥QT  	Ò4Qd‘Ùd×GgW•�UÕ§U­V©±”è¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M²¤…òÑ³àG=½Î*‡ïdQßäO}�T“R6ÕŸŽë¥ën¸;86�#£š˜;Æ±îÑõn±5eö½*çt'k†�<ûaŽ

¾Ž N'Q/Ò68Šë¾è!šÏhFO<òàg÷PaiQ{ÐJ§PwçÕJpMA…ÿLwµOof xâ“‚ú�4©»~?ÔWN¨*ô)OãøSÛHBë]u+Ý¶�4€›³È!†ë†ëM†øŽ3ÛÍñ‚ÎL©^”è'ÄìÞKÒ �k�½7H0ó±.DÃÒÂM‚P1â·À �Ûà±81É\=Ô­2/@Äcßœ58…+n7ßN?#ø?²‡ç�#­<çü}ØSMÇ®µ/=�Årô7Û®µ5çQüÏ…+1ßà…ólN±ëPAÃ]¾ ÝN�y×OuË™¶M\6Ô!zÔo=×lAÃL�;<}çÓEßý°Çò8ãñ×Þ~KÏá5GýqÌmØ/Ž¬‡Ï<Þì6‰Ô—¼J\ËH,ˆ"-) T¿ÐïôÐÑI�ScnÖ’Ó_B T¿ÐïôÐÑI�ScnÖ’Ó_d¿?åï?oÏù{ÏÒ¼ý®óýƒ‡–ª©©�ñ>ÃîšôRHAª��ê�U:}‡Ä�OÜ#>Ï1Ç/ô'7û<ó+<yÿ=Ì÷|±¼=Ûß�Eî¾ÜÌ°;°Ô"ÌpÏ0ƒ�÷<2Å0¬ñÌØ<J
ü8<Í5Ñ�Cïƒ<ßÔó ¯ƒß,2ìü}ô@¸3Ë#¿Á C3À#óëàGï3ü×ïúçÃ­òß<rÄ#ñAó7à�‡óSð�‡??Ü4ÏÇÅA3qÿclô±Ö×î�V¢	®—˜£¾©ÎbsŽ¥�¨›cš:ü˜:¶«¾ú&Vø(âî¦J ü¢Ð× È"7ˆ¨áÍ÷‚€6�}ø´ï«Àz³«ÿ¿š=Ãí³Ï�£óŽ@;(áÖ¼‚(°8rú½ƒŽÏˆ£ß�®3ËëŽrþ°þÖôÁúúüÃã�ãºó.³ë?´q,¿¨>ÿ>¬NÂ~¥Xzu8‘ºOz©TU �@å­ï×uŽª:'€»¸)Fá>;¸9yxéOµ•:>„Žµ$ˆ�ËŠ"à—‘ûGú¤´ˆnäÒI+bÛ¦šÒjeèÕ…›DHD¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¦Ø¸;“›óþ^óöüÿ—¼ý+ÏÚï?Ø8yjªš˜Ñóì>á9¯E$„ê¨ùÉýk?™!,šs3·ƒË*�rÈx_àÊ^–Ï£MÛÿ«=82Y1D?7ÆÏëõÒŒÓá`Å€s?‰0Í¥3‚
s4È|çz=3ù3ó¾¬êëúú»=ÊÏ¯òÔîVÀþ¤
ñ©#©Ã~ÇýUz@†
Ü’Û?†G
«?¨r�/rº–Hè¯W�uÞ×ù»šê†¦P£Æ¬>h)Ua8j]U©s©uY¥Î�jBê°b3ÿšŠš«•�­Y‰¼í„u—øec­ešPQ&k]«È³úkúª0Ú£ëâª;Es‚%QŽm(ÜH
vÅàn×L%èDªÏñEÊ¯Xa7ªT®9ˆ3	¦üšð5^ÂÏ6wší‚„žßKtU6éÁƒaÒQ˜Ò8ö_a!—xÃ×§®q°AñA9|ê¨Ýv b^Ï¡²€_áaOgÕÇ!ßq�‰5ƒ]rŒÕæ—ƒžžäëð×ZY0eJ4zã;ëÃƒ‚¦Ž[�<!2ìÂm£MàF	OïÉ²Jnw-y�…ÅdtláûxiM —‹GÔ©í™ž¤hjUëj¥©T�…ê¡–í‡Õoér«+Þ-òEQµAºèFúª¸Ì–ãŽ�]U_|çÓ**<Q9õîªñB^´Ñ3F}Yâºä‰<’�÷Þµ¸“|ùÙû;Ž[âŸH8ãÒe,‹§À {QÆJ|C4ÂÀáp ¬�Ý?N�1qùß„gçL„æS=Î¿T0t&ð”Ã@°]LÜ@N½ Ï‚n¦KâÀ«=û‡¡2Æª!ŽË@ù�UÏMsªœ+àú£ƒêŽÝŠêøÊoøkˆ|š|ú„„áŸ;<ÓÅÇÄÍ1-!1     ¬    6  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  1$<?xml version="1.0"?><Features nextValidID="31"><Feature recentID="30" name="MCS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3502-3609" color="#ccffcc" type="standard"/></Feature><Feature recentID="29" name="linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4359" maxFusedRunOn="4359"><Segment range="3625-3639" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="28" name="linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4359" maxFusedRunOn="4359"><Segment range="3610-3624" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="27" name="Cd9-m" directionality="1" translationMW="25258.64" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3513" maxFusedRunOn="3513"><Segment range="2824-3501" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="26" name="Factor Xa site" directionality="2" translationMW="473.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5884" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="5501" maxFusedRunOn="5501" detectionMode="exactProteinMatch"><Segment range="5885-5896" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="25" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6739-6755" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="M13 rev" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="211-227" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="187-203" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="T7 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6711-6729" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="T3 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="248-266" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T3 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="CAP binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="113-134" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="lac promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-35" range="149-154" color="#ffffff" type="standard"/><Segment range="155-172" color="#ffffff" type="standard"/><Segment name="-10" range="173-179" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="AmpR promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7378-7482" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="cPPT/CTS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2095-2211" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;central polypurine tract and central termination sequence of HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6394-6515" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="HIV-1 Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="748-873" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of human immunodeficiency virus type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6555-6690" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4589-4724" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="521-701" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2538-2741" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="RSV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="294-520" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Rous sarcoma virus enhancer/promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="3' LTR (Î”U3)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6089-6322" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;self-inactivating&amp;nbsp;3' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="RRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1366-1599" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2234-2537" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4409-4738" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6897-7352" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8514-9102" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5414-6002" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="4748-5347" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="1" name="EGFP" directionality="1" translationMW="26942.08" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="3640-3642" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="3643-3645" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="3646-4359" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="AmpR" directionality="1" translationMW="31558.16" type="CDS" allowSegmentOverlaps="0" cleavageArrows="7551" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment name="signal sequence" range="7483-7551" color="#ccffcc" type="standard" translated="1"/><Segment range="7552-8343" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>9d09dad2-79cd-4df1-af32-0dbde0e69422</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:9:52">2018.9.13</Created>
<LastModified UTC="3:3:39">2022.7.20</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   ¯<StrandColors><TopStrand><ColorRange range="3501..3638" colors="black"/></TopStrand><BottomStrand><ColorRange range="3501..3638" colors="black"/></BottomStrand></StrandColors>  =P     	  #ì        #ìÁDÏÖsWÏ»üÌ ñ­¥�87
¿L!3\Ö–!);{iÿC©Þ¦:ðì˜¢ Z!0eÖ­Ýtæa§=LÍi·•uS�8ÔébK¦5žè’è×`—JMv>�AU7ÈØñ �IB	ÆÈúitp.� q}áhóhÑ¢jR>NÆvWîà¤}„øîàåäýã¥>ÖTèú:Ô’"?.(ˆ{‚^Doíê’Ò U»“H3ñt¡S_D»±Í°èáLÍÃQ@0Ç‚sÕZ¡äÁED`#2ÒiL E¤lÆUk
Pô TUY•¥]™&S@NEÆ³J[àø¤Wm8‘ÕžÓ�}¿­ÑEéi™gI5þæˆ�µA‘TwÔêGQ‘UÕIGß7ML<áºÑøA„ZR™•™RIU¡}¤MýõQH#DEUH�N¢¾€®€M4Þ`3Lµ‡8p�GZh¸&HÓMZŽ9¡×M¸£^Ý. ÛSNô5ø8�QŸ•×Nø¨ŽàUí¨ßxâ>�c’�%•»�tj·qøÑ¥Ö�Ôég{éhQlÕ}5E…ÔZh‘•€Ô¨�h*]—Vƒˆ&eZke†I:	
”Y*£‰êæHY�Ð­ö›*ÑýûõÿñÐüPYQBDGDtokH\±Âl)z¥QT  	Ò4Qd‘Ùd×GgW•�UÕ§U­V©±”è¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M²¤…òÑ³àG=½Î*‡ïdQßäO}�T“R6ÕŸŽë¥ën¸;86�#£š˜;Æ±îÑõn±5eö½*çt'k†�<ûaŽ

¾Ž N'Q/Ò68Šë¾è!šÏhFO<òàg÷PaiQ{ÐJ§PwçÕJpMA…ÿLwµOof xâ“‚ú�4©»~?ÔWN¨*ô)OãøSÛHBë]u+Ý¶�4€›³È!†ë†ëM†øŽ3ÛÍñ‚Å®bÄö Ÿ CH C	6M@ÄŠß ‚?o‚ÄàpÇ%p÷R´È¼11�~pÔáþ­¸ß|58ü�àýþÈ}þœ24Ž´óŸñ÷aM7ºÔ¼ö?9ËÐLßnºÔp×�Gó<B¬@Ç‚Í±8P6Ç­Avøu8uç]=×.fØ<u4QpÛP…ëQ¼÷]±24ìñ÷ŸMöÃ?Èã�Ç_yøMý/?„ÕõÄS1·`¾8²<ð{°ØN'R\ðy)s,} ±þ Xˆ´¤�RÿC¿ÓCE&5M�»ZKM}�RÿC¿ÓCE&5M�»ZKM}’üÿ—¼ý¿?åï?Jóö»ÏöZª¦¦4CÄ<û¸NkÑI!:ª>Cª5Téöu?pŒû=üÇ¿Ð<œßìóÌ¬ñçü÷3ÝòÆð|÷lL|N5ºûs2ÀìvÃPˆ0qÃ<Â.ÜðDËÀ"³Äl?3`ñ(L+ðàó4×F¾ó|?POÌ‚¾|°Ë³ñ÷Ð5à4Ï,Žÿ�<LÏ �Ï¯�¼Ïó_¿ëœL;·Ë|ñË�ÅÌß‚6ÍOÂ>üÿqüÓ?ÍÇý�³ÒÇ[_¸uZˆThx&º^bŽú§9ˆÎ:–>¢mŽhëò`êÚ®ûè™[à£‹º˜A(ƒò‹C\ƒ ˆÞ"£‡5ÿÞ
 Ú=÷âÓ¾¯êÎ¯þþh÷¶Ï>>�Î8 ì8 7‡Zò¢ÀáËêö;>"�~¸Ì/®9ËúÃû[ÓëëóŽ<;Œ>ëÌºÏ¬þÑÄ°nþ <ûüø±;	ú”}aéÔ4âFé=ê¥QT –·¿\QÔn:¨èžîà¤}„øîàåäýã¥>ÖTèú:Ô’"?.(ˆ{‚^Doíê’Ò U»“I$­‹nškI©—£TVm!^Šš4é–
ueMdÛujÝYSjªÞ6¹(‚¢õ{mbn›bàîNoÏù{ÏÛóþ^óô¯?k¼ÿ`áåªªjcD<CÏ°û„æ½’Cª Cä'õ¬þd„²iÌÎÞ,ªuË!àƒ)ýz[>�7oþ¬ôàÉdÅüß?¯×J3O…ƒÌþ$Ã6”Ì0")ÌÓ!ó�èôÏäÏÎú³«¯ëêì÷+<:¿ËS¹[ ;ú�+Æ¤Ž§ûõUèi+rHlþ*¬þ¡Ê½ÊêY {¢½^×{_æì.kª™@j�°ù ¥U„á©uV¥ÌZ¥Õf—:u©7ªÀˆÌþj*j®Vvµf&ó¶Ö_á•Žµ–hUAD˜I­v¯"Ïé¯ê¨Ãj�¯Š¨íýÎT•F8Q´ #q )Û�»]0”o¡«?Å*½a„Þ©R¸æ Ì&›òhsÀÔM{<ÙÞk´Xz
{}-ÑTÛ§‡IDZcHãÙ}„†]ã^ž¸uÆÁÅåóª£uØ m‰{>†Ê|……=ŸWL‡}Æ>$ÖuÊ3Wš^^z{“¯Ã]idxÁ•(Ð}ëŒì?¯
š8enð„È³	¶�7€=%?¿&É)¹Üp´5æ‘Ñ³„d#íá¥6‚^-R§¶fz‘¡©W­ª–¥PnBoª†[¶U¿¥ÈJ¬¯xL´ÉFÕë¡êªã2[Ž:5uU}óŸL ¨¨ðmDç×º«Å	x:ÓDÍõgŠë’$òJCßzÔ^âMóçgìî9oŠ} ã�H1”².Ÿ�íG)ðeÓ…À²7tý:ÅÇç|^Ÿ�2˜EL÷:üPPÁÐ›ÂSÁt3q:ô W>	º™/‹¬÷î„Ë¨†;- æW=5Ì>ªp¯ƒêŽª;v+«ã)¿á® eòióê†|ìóO4Ä´„Ä    ¬  È  å  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  0k<?xml version="1.0"?><Features nextValidID="30"><Feature recentID="29" name="linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4278" maxFusedRunOn="4278"><Segment range="3544-3558" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="28" name="linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4278" maxFusedRunOn="4278"><Segment range="3529-3543" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="27" name="Cd9-m" directionality="1" translationMW="25258.64" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4278" maxFusedRunOn="4278"><Segment range="2824-3501" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="26" name="Factor Xa site" directionality="2" translationMW="473.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5803" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="5420" maxFusedRunOn="5420" detectionMode="exactProteinMatch"><Segment range="5804-5815" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="25" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6658-6674" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="M13 rev" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="211-227" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="187-203" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="T7 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6630-6648" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="T3 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="248-266" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T3 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="CAP binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="113-134" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="lac promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-35" range="149-154" color="#ffffff" type="standard"/><Segment range="155-172" color="#ffffff" type="standard"/><Segment name="-10" range="173-179" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="AmpR promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7297-7401" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="cPPT/CTS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2095-2211" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;central polypurine tract and central termination sequence of HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6313-6434" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="HIV-1 Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="748-873" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of human immunodeficiency virus type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6474-6609" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4508-4643" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="521-701" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2538-2741" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="RSV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="294-520" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Rous sarcoma virus enhancer/promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="3' LTR (Î”U3)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6008-6241" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;self-inactivating&amp;nbsp;3' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="RRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1366-1599" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2234-2537" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4328-4657" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6816-7271" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8433-9021" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5333-5921" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="4667-5266" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="1" name="EGFP" directionality="1" translationMW="26942.08" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="3559-3561" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="3562-3564" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="3565-4278" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="AmpR" directionality="1" translationMW="31558.16" type="CDS" allowSegmentOverlaps="0" cleavageArrows="7470" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment name="signal sequence" range="7402-7470" color="#ccffcc" type="standard" translated="1"/><Segment range="7471-8262" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>9d09dad2-79cd-4df1-af32-0dbde0e69422</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:9:52">2018.9.13</Created>
<LastModified UTC="3:3:13">2022.7.20</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   ¯<StrandColors><TopStrand><ColorRange range="3528..3557" colors="black"/></TopStrand><BottomStrand><ColorRange range="3528..3557" colors="black"/></BottomStrand></StrandColors>  =B     	  #Ñ        #ÑÁDÏÖsWÏ»üÌ ñ­¥�87
¿L!3\Ö–!);{iÿC©Þ¦:ðì˜¢ Z!0eÖ­Ýtæa§=LÍi·•uS�8ÔébK¦5žè’è×`—JMv>�AU7ÈØñ �IB	ÆÈúitp.� q}áhóhÑ¢jR>NÆvWîà¤}„øîàåäýã¥>ÖTèú:Ô’"?.(ˆ{‚^Doíê’Ò U»“H3ñt¡S_D»±Í°èáLÍÃQ@0Ç‚sÕZ¡äÁED`#2ÒiL E¤lÆUk
Pô TUY•¥]™&S@NEÆ³J[àø¤Wm8‘ÕžÓ�}¿­ÑEéi™gI5þæˆ�µA‘TwÔêGQ‘UÕIGß7ML<áºÑøA„ZR™•™RIU¡}¤MýõQH#DEUH�N¢¾€®€M4Þ`3Lµ‡8p�GZh¸&HÓMZŽ9¡×M¸£^Ý. ÛSNô5ø8�QŸ•×Nø¨ŽàUí¨ßxâ>�c’�%•»�tj·qøÑ¥Ö�Ôég{éhQlÕ}5E…ÔZh‘•€Ô¨�h*]—Vƒˆ&eZke†I:	
”Y*£‰êæHY�Ð­ö›*ÑýûõÿñÐüPYQBDGDtokH\±Âl)z¥QT  	Ò4Qd‘Ùd×GgW•�UÕ§U­V©±”è¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M²¤…òÑ³àG=½Î*‡ïdQßäO}�T“R6ÕŸŽë¥ën¸;86�#£š˜;Æ±îÑõn±5eö½*çt'k†�<ûaŽ

¾Ž N'Q/Ò68Šë¾è!šÏhFO<òàg÷PaiQ{ÐJ§PwçÕJpMA…ÿLwµOof xâ“‚ú�4©»~?ÔWN¨*ô)OãøSÛHBë]u+Ý¶�4€›³È!†ë†ëM†øŽ3ÛÍñ‚ÃÒÂM‚P1â·À �Ûà±81É\=Ô­2/@Äcßœ58…+n7ßN?#ø?²‡ç�#­<çü}ØSMÇ®µ/=�Årô7Û®µ5çQüÏ…+1ßà…ólN±ëPAÃ]¾ ÝN�y×OuË™¶M\6Ô!zÔo=×lAÃL�;<}çÓEßý°Çò8ãñ×Þ~KÏá5GýqÌmØ/Ž¬‡Ï<Þì6‰Ô—¼J\ËH,ˆ"-) T¿ÐïôÐÑI�ScnÖ’Ó_B T¿ÐïôÐÑI�ScnÖ’Ó_d¿?åï?oÏù{ÏÒ¼ý®óýƒ‡–ª©©�ñ>ÃîšôRHAª��ê�U:}‡Ä�OÜ#>Ï1Ç/ô'7û<ó+<yÿ=Ì÷|±¼=Ûß�Eî¾ÜÌ°;°Ô"ÌpÏ0ƒ�÷<2Å0¬ñÌØ<J
ü8<Í5Ñ�Cïƒ<ßÔó ¯ƒß,2ìü}ô@¸3Ë#¿Á C3À#óëàGï3ü×ïúçÃ­òß<rÄ#ñAó7à�‡óSð�‡??Ü4ÏÇÅA3qÿclô±Ö×î�V¢	®—˜£¾©ÎbsŽ¥�¨›cš:ü˜:¶«¾ú&Vø(âî¦J ü¢Ð× È"7ˆ¨áÍ÷‚€6�}ø´ï«Àz³«ÿ¿š=Ãí³Ï�£óŽ@;(áÖ¼‚(°8rú½ƒŽÏˆ£ß�®3ËëŽrþ°þÖôÁúúüÃã�ãºó.³ë?´q,¿¨>ÿ>¬NÂ~¥Xzu8‘ºOz©TU �@å­ï×uŽª:'€»¸)Fá>;¸9yxéOµ•:>„Žµ$ˆ�ËŠ"à—‘ûGú¤´ˆnäÒI+bÛ¦šÒjeèÕ…›DHD¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¦Ø¸;“›óþ^óöüÿ—¼ý+ÏÚï?Ø8yjªš˜Ñóì>á9¯E$„ê¨ùÉýk?™!,šs3·ƒË*�rÈx_àÊ^–Ï£MÛÿ«=82Y1D?7ÆÏëõÒŒÓá`Å€s?‰0Í¥3‚
s4È|çz=3ù3ó¾¬êëúú»=ÊÏ¯òÔîVÀþ¤
ñ©#©Ã~ÇýUz@†
Ü’Û?†G
«?¨r�/rº–Hè¯W�uÞ×ù»šê†¦P£Æ¬>h)Ua8j]U©s©uY¥Î�jBê°b3ÿšŠš«•�­Y‰¼í„u—øec­ešPQ&k]«È³úkúª0Ú£ëâª;Es‚%QŽm(ÜH
vÅàn×L%èDªÏñEÊ¯Xa7ªT®9ˆ3	¦üšð5^ÂÏ6wší‚„žßKtU6éÁƒaÒQ˜Ò8ö_a!—xÃ×§®q°AñA9|ê¨Ýv b^Ï¡²€_áaOgÕÇ!ßq�‰5ƒ]rŒÕæ—ƒžžäëð×ZY0eJ4zã;ëÃƒ‚¦Ž[�<!2ìÂm£MàF	OïÉ²Jnw-y�…ÅdtláûxiM —‹GÔ©í™ž¤hjUëj¥©T�…ê¡–í‡Õoér«+Þ-òEQµAºèFúª¸Ì–ãŽ�]U_|çÓ**<Q9õîªñB^´Ñ3F}Yâºä‰<’�÷Þµ¸“|ùÙû;Ž[âŸH8ãÒe,‹§À {QÆJ|C4ÂÀáp ¬�Ý?N�1qùß„gçL„æS=Î¿T0t&ð”Ã@°]LÜ@N½ Ï‚n¦KâÀ«=û‡¡2Æª!ŽË@ù�UÏMsªœ+àú£ƒêŽÝŠêøÊoøkˆ|š|ú„„áŸ;<ÓÅÇÄÍ1-!1     Ê  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  0k<?xml version="1.0"?><Features nextValidID="30"><Feature recentID="29" name="linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4251" maxFusedRunOn="4251"><Segment range="3517-3531" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="28" name="linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4251" maxFusedRunOn="4251"><Segment range="3502-3516" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="27" name="Cd9-m" directionality="1" translationMW="25258.64" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4251" maxFusedRunOn="4251"><Segment range="2824-3501" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="26" name="Factor Xa site" directionality="2" translationMW="473.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5776" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="5393" maxFusedRunOn="5393" detectionMode="exactProteinMatch"><Segment range="5777-5788" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="25" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6631-6647" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="M13 rev" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="211-227" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="187-203" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="T7 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6603-6621" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="T3 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="248-266" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T3 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="CAP binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="113-134" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="lac promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-35" range="149-154" color="#ffffff" type="standard"/><Segment range="155-172" color="#ffffff" type="standard"/><Segment name="-10" range="173-179" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="AmpR promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7270-7374" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="cPPT/CTS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2095-2211" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;central polypurine tract and central termination sequence of HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6286-6407" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="HIV-1 Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="748-873" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of human immunodeficiency virus type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6447-6582" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4481-4616" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="521-701" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2538-2741" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="RSV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="294-520" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Rous sarcoma virus enhancer/promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="3' LTR (Î”U3)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5981-6214" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;self-inactivating&amp;nbsp;3' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="RRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1366-1599" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2234-2537" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4301-4630" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6789-7244" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8406-8994" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5306-5894" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="4640-5239" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="1" name="EGFP" directionality="1" translationMW="26942.08" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="3532-3534" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="3535-3537" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="3538-4251" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="AmpR" directionality="1" translationMW="31558.16" type="CDS" allowSegmentOverlaps="0" cleavageArrows="7443" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment name="signal sequence" range="7375-7443" color="#ccffcc" type="standard" translated="1"/><Segment range="7444-8235" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>9d09dad2-79cd-4df1-af32-0dbde0e69422</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:9:52">2018.9.13</Created>
<LastModified UTC="3:2:28">2022.7.20</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   ¯<StrandColors><TopStrand><ColorRange range="3501..3530" colors="black"/></TopStrand><BottomStrand><ColorRange range="3501..3530" colors="black"/></BottomStrand></StrandColors>  9±     	  #³        #³ÁDÏÖsWÏ»üÌ ñ­¥�87
¿L!3\Ö–!);{iÿC©Þ¦:ðì˜¢ Z!0eÖ­Ýtæa§=LÍi·•uS�8ÔébK¦5žè’è×`—JMv>�AU7ÈØñ �IB	ÆÈúitp.� q}áhóhÑ¢jR>NÆvWîà¤}„øîàåäýã¥>ÖTèú:Ô’"?.(ˆ{‚^Doíê’Ò U»“H3ñt¡S_D»±Í°èáLÍÃQ@0Ç‚sÕZ¡äÁED`#2ÒiL E¤lÆUk
Pô TUY•¥]™&S@NEÆ³J[àø¤Wm8‘ÕžÓ�}¿­ÑEéi™gI5þæˆ�µA‘TwÔêGQ‘UÕIGß7ML<áºÑøA„ZR™•™RIU¡}¤MýõQH#DEUH�N¢¾€®€M4Þ`3Lµ‡8p�GZh¸&HÓMZŽ9¡×M¸£^Ý. ÛSNô5ø8�QŸ•×Nø¨ŽàUí¨ßxâ>�c’�%•»�tj·qøÑ¥Ö�Ôég{éhQlÕ}5E…ÔZh‘•€Ô¨�h*]—Vƒˆ&eZke†I:	
”Y*£‰êæHY�Ð­ö›*ÑýûõÿñÐüPYQBDGDtokH\±Âl)z¥QT  	Ò4Qd‘Ùd×GgW•�UÕ§U­V©±”è¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M²¤…òÑ³àG=½Î*‡ïdQßäO}�T“R6ÕŸŽë¥ën¸;86�#£š˜;Æ±îÑõn±5eö½*çt'k†�<ûaŽ

¾Ž N'Q/Ò68Šë¾è!šÏhFO<òàg÷PaiQ{ÐJ§PwçÕJpMA…ÿLwµOof xâ“‚ú�4©»~?ÔWN¨*ô)OãøSÛHBë]u+Ý¶�4€›³È!†ë†ëM†øŽ3ÛÍñ‚Ø!5N+|ý¾�Ã•ÃÝJÓ"ðÄ@Æ=ùÃS‡øR¶ã}ðÔãò?ƒ÷û!÷ø~pÈÒ:ÓÎÇÝ…4ÜzëRóØüPç/A3}ºëPqÃ^uÌñR±þ_6Äá@Ûµ5ÛàÔàI×�t÷\¹›`ñÔÑEÃmB­FóÝvÄ4ÈÓ³ÇÞ}4]ÿÛpÿ#Ž?}çá7ô¼þT×LÆÝ‚øàJÈ|óÀmîÃ`q8�IpÁä¥Ì±ô‚Çø�b"Ò�"KýÿM˜Õ66íi-5ô"KýÿM˜Õ66íi-5öKóþ^óöüÿ—¼ý+ÏÚï?Ø8yjªš˜Ñóì>á9¯E$„ê¨ù¨ÕS§Ø|IÔýÂ3ì÷órÿ@òs³Ï2³ÇŸóÜÏwËÁóÝ±0-ñ8Ô^ëíÌË±ÛB ,ÁÇó0¸sÁ,S 0ŠÏ°üÍƒÄ¡0¯ÃƒÌÓ]>ø3ÍðýA?2
ø=òÃ.ÏÇß@Ô€Ó<²;ü0ñ3<?>¾~ó?Í~ÿ®q0ì:ß-óÇ,B?3~Ø5?øsóýÇóLü|T7ö6ÏKm~àiÕj!Q¡àšéyŠ;êœæ g8êXú‰¶9£¯Éƒ«j»ï¢eo‚Ž.êa¢Ê-r‚#xŠŽ×ÿx(h÷ß‹Nú¼«:¿ûù£Ü0^Û<øú?8àt°â€ÞkÈ"‹ S‡/«Ø8ìøŠ=øã0¾¸ç/ëíoL¯¯Ì>8ðî0û¯2ë>³ûGÁ»ú€óïóàZÄì'êQõ‡§PÓ‰¤÷ª•EP xZÞýqGQ¸ê£¢x»‚‘ônà»ƒ—�÷Ž”ûYS£èHëRHˆü¸¢!î	y¿´ªKH�VîM$’¶-ºi­&¦^�QXY´D„Az*hÓ¦X)Õ•5“mÕ«ueMª«xÚä¢
‹Õíµ‰ºm‹ƒ¹9¿?åï?oÏù{ÏÒ¼ý®óýƒ‡–ª©©�ñ>ÃîšôRHAª��ŸÖ³ù’É§3;x<²©×,‡€Uþ§õélú4Ý¿ú³Óƒ%“Có|lþ¿](Í>X3ø“ÚS0Àˆ §3L‡Îw£Ó?“?;êÎ®¿¯«³Ü¬ðêÿ-Nål ïê@¯’:œ7ìÕW¡¤`­É =³ødpª³ú‡(÷+©d�îŠõx]í›°¹®¨jeª<jÃæ‚•V†¥ÕZ—1j—Uš\éÖ¤ Þ« V#0_ù¨©ª¹YÚÕ˜›ÎØGY†V:ÖY¡Ua&µÚ¼‹?¦¿ª£ª>¾*£·ôW8!RUáFÒ€�Ä€§l^ítÂQ¾„J¬ÿ\ªõ†z¥Jã˜ƒ0šoÉ¡ÏQ5ì,ógy®Ñaè(Iíô·ESnœ6%i�#�eöwŒ=zzá×—Îª�×`¶%ìú(ðNö}\q2÷ø“X5×(Í^ix1yéîN¿u¥‘à`CT£A÷®3°þ¼88*há•¸Â .Ì&Ú4Þ ô`”þü›$¦çqÀÐ×˜\VGFÎ��·†”Ú	x´}JžÙ™êF†¥^¶ªZ•A¹Q¾ªnØ}Vþ—!*²½á2Ñÿ$UT®„oª«ŒÉn8èÕÕU÷Î}0‚¢£ÁµŸ^ê¯%àëM4gÕž+®H“É)}ëQ{‰7Ï�Ÿ³¸å¾)ôƒŽ= ÆRÈº|
µd§Á”3L, 
ÈÝÓôè�ñxF~tÈNa3Üëñ@ECBo	L4ÐDÍÄëÐ\ø&êd¾,
³ß¸z,j¢ì´ 0O˜\ô×0ú©Â¾ª8>¨íØ®¯Œ¦ÿ†¸�—É§Ï¨HNó³Í<\|LÓÒ    ¬  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  -–<?xml version="1.0"?><Features nextValidID="28"><Feature recentID="27" name="Cd9-m" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4221" maxFusedRunOn="4221"><Segment range="2824-3501" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="26" name="Factor Xa site" directionality="2" translationMW="473.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5746" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="5363" maxFusedRunOn="5363" detectionMode="exactProteinMatch"><Segment range="5747-5758" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="25" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6601-6617" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="M13 rev" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="211-227" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="187-203" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="T7 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6573-6591" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="T3 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="248-266" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T3 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="CAP binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="113-134" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="lac promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-35" range="149-154" color="#ffffff" type="standard"/><Segment range="155-172" color="#ffffff" type="standard"/><Segment name="-10" range="173-179" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="AmpR promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7240-7344" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="cPPT/CTS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2095-2211" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;central polypurine tract and central termination sequence of HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6256-6377" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="HIV-1 Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="748-873" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of human immunodeficiency virus type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6417-6552" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4451-4586" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="521-701" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2538-2741" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="RSV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="294-520" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Rous sarcoma virus enhancer/promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="3' LTR (Î”U3)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5951-6184" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;self-inactivating&amp;nbsp;3' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="RRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1366-1599" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2234-2537" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4271-4600" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6759-7214" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8376-8964" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5276-5864" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="4610-5209" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="1" name="EGFP" directionality="1" translationMW="26942.08" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="3502-3504" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="3505-3507" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="3508-4221" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="AmpR" directionality="1" translationMW="31558.16" type="CDS" allowSegmentOverlaps="0" cleavageArrows="7413" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment name="signal sequence" range="7345-7413" color="#ccffcc" type="standard" translated="1"/><Segment range="7414-8205" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>9d09dad2-79cd-4df1-af32-0dbde0e69422</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:9:52">2018.9.13</Created>
<LastModified UTC="3:1:29">2022.7.20</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  7¸      T  !         !ÁDÏÖsWÏ»üÌ ñ­¥�87
¿L!3\Ö–!);{iÿC©Þ¦:ðì˜¢ Z!0eÖ­Ýtæa§=LÍi·•uS�8ÔébK¦5žè’è×`—JMv>�AU7ÈØñ �IB	ÆÈúitp.� q}áhóhÑ¢jR>NÆvWîà¤}„øîàåäýã¥>ÖTèú:Ô’"?.(ˆ{‚^Doíê’Ò U»“H3ñt¡S_D»±Í°èáLÍÃQ@0Ç‚sÕZ¡äÁED`#2ÒiL E¤lÆUk
Pô TUY•¥]™&S@NEÆ³J[àø¤Wm8‘ÕžÓ�}¿­ÑEéi™gI5þæˆ�µA‘TwÔêGQ‘UÕIGß7ML<áºÑøA„ZR™•™RIU¡}¤MýõQH#DEUH�N¢¾€®€M4Þ`3Lµ‡8p�GZh¸&HÓMZŽ9¡×M¸£^Ý. ÛSNô5ø8�QŸ•×Nø¨ŽàUí¨ßxâ>�c’�%•»�tj·qøÑ¥Ö�Ôég{éhQlÕ}5E…ÔZh‘•€Ô¨�h*]—Vƒˆ&eZke†I:	
”Y*£‰êæHY�Ð­ö›*ÑýûõÿñÐüPYQBDGDtokH\±Âl)z¥QT  	Ò4Qd‘Ùd×GgW•�UÕ§U­V©±”è¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M²¤…òÑ³àG=½Î*‡ïdQßäX!5N+|ý¾�Ã•ÃÝJÓ"ðÄ@Æ=ùÃS‡øR¶ã}ðÔãò?ƒ÷û!÷ø~pÈÒ:ÓÎÇÝ…4ÜzëRóØüPç/A3}ºëPqÃ^uÌñR±þ_6Äá@Ûµ5ÛàÔàI×�t÷\¹›`ñÔÑEÃmB­FóÝvÄ4ÈÓ³ÇÞ}4]ÿÛpÿ#Ž?}çá7ô¼þT×LÆÝ‚øàJÈ|óÀmîÃ`q8�IpÁä¥Ì±ô‚Çø�b"Ò�"KýÿM˜Õ66íi-5ô"KýÿM˜Õ66íi-5öKóþ^óöüÿ—¼ý+ÏÚï?Ø8yjªš˜Ñóì>á9¯E$„ê¨ù¨ÕS§Ø|IÔýÂ3ì÷órÿ@òs³Ï2³ÇŸóÜÏwËÁóÝ±0-ñ8Ô^ëíÌË±ÛB ,ÁÇó0¸sÁ,S 0ŠÏ°üÍƒÄ¡0¯ÃƒÌÓ]>ø3ÍðýA?2
ø=òÃ.ÏÇß@Ô€Ó<²;ü0ñ3<?>¾~ó?Í~ÿ®q0ì:ß-óÇ,B?3~Ø5?øsóýÇóLü|T7ö6ÏKm~àiÕj!Q¡àšéyŠ;êœæ g8êXú‰¶9£¯Éƒ«j»ï¢eo‚Ž.êa¢Ê-r‚#xŠŽ×ÿx(h÷ß‹Nú¼«:¿ûù£Ü0^Û<øú?8àt°â€ÞkÈ"‹ S‡/«Ø8ìøŠ=øã0¾¸ç/ëíoL¯¯Ì>8ðî0û¯2ë>³ûGÁ»ú€óïóàZÄì'êQõ‡§PÓ‰¤÷ª•EP xZÞýqGQ¸ê£¢x»‚‘ônà»ƒ—�÷Ž”ûYS£èHëRHˆü¸¢!î	y¿´ªKH�VîM$’¶-ºi­&¦^�QXY´D„Az*hÓ¦X)Õ•5“mÕ«ueMª«xÚä¢
‹Õíµ‰ºm‹ƒ¹9¿?åï?oÏù{ÏÒ¼ý®óýƒ‡–ª©©�ñ>ÃîšôRHAª��ŸÖ³ù’É§3;x<²©×,‡€Uþ§õélú4Ý¿ú³Óƒ%“Có|lþ¿](Í>X3ø“ÚS0Àˆ §3L‡Îw£Ó?“?;êÎ®¿¯«³Ü¬ðêÿ-Nål ïê@¯’:œ7ìÕW¡¤`­É =³ødpª³ú‡(÷+©d�îŠõx]í›°¹®¨jeª<jÃæ‚•V†¥ÕZ—1j—Uš\éÖ¤ Þ« V#0_ù¨©ª¹YÚÕ˜›ÎØGY†V:ÖY¡Ua&µÚ¼‹?¦¿ª£ª>¾*£·ôW8!RUáFÒ€�Ä€§l^ítÂQ¾„J¬ÿ\ªõ†z¥Jã˜ƒ0šoÉ¡ÏQ5ì,ógy®Ñaè(Iíô·ESnœ6%i�#�eöwŒ=zzá×—Îª�×`¶%ìú(ðNö}\q2÷ø“X5×(Í^ix1yéîN¿u¥‘à`CT£A÷®3°þ¼88*há•¸Â .Ì&Ú4Þ ô`”þü›$¦çqÀÐ×˜\VGFÎ��·†”Ú	x´}JžÙ™êF†¥^¶ªZ•A¹Q¾ªnØ}Vþ—!*²½á2Ñÿ$UT®„oª«ŒÉn8èÕÕU÷Î}0‚¢£ÁµŸ^ê¯%àëM4gÕž+®H“É)}ëQ{‰7Ï�Ÿ³¸å¾)ôƒŽ= ÆRÈº|
µd§Á”3L, 
ÈÝÓôè�ñxF~tÈNa3Üëñ@ECBo	L4ÐDÍÄëÐ\ø&êd¾,
³ß¸z,j¢ì´ 0O˜\ô×0ú©Â¾ª8>¨íØ®¯Œ¦ÿ†¸�—É§Ï¨HNó³Í<\|LÓÒ  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ,P<?xml version="1.0"?><Features nextValidID="27"><Feature recentID="26" name="Factor Xa site" directionality="2" translationMW="473.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5062" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="4679" maxFusedRunOn="4679" detectionMode="exactProteinMatch"><Segment range="5063-5074" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="25" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5917-5933" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="M13 rev" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="211-227" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="187-203" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="T7 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5889-5907" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="T3 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="248-266" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T3 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="CAP binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="113-134" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="lac promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-35" range="149-154" color="#ffffff" type="standard"/><Segment range="155-172" color="#ffffff" type="standard"/><Segment name="-10" range="173-179" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="AmpR promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6556-6660" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="cPPT/CTS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2095-2211" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;central polypurine tract and central termination sequence of HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5572-5693" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="HIV-1 Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="748-873" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of human immunodeficiency virus type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5733-5868" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3767-3902" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="521-701" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2538-2741" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="RSV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="294-520" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Rous sarcoma virus enhancer/promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="3' LTR (Î”U3)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5267-5500" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;self-inactivating&amp;nbsp;3' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="RRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1366-1599" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2234-2537" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3587-3916" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6075-6530" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7692-8280" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4592-5180" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3926-4525" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="1" name="EGFP" directionality="1" translationMW="26942.08" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="2818-2820" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="2821-2823" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="2824-3537" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="AmpR" directionality="1" translationMW="31558.16" type="CDS" allowSegmentOverlaps="0" cleavageArrows="6729" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment name="signal sequence" range="6661-6729" color="#ccffcc" type="standard" translated="1"/><Segment range="6730-7521" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>9d09dad2-79cd-4df1-af32-0dbde0e69422</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:9:52">2018.9.13</Created>
<LastModified UTC="3:0:29">2022.7.20</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  `ý7zXZ  æÖ´F !   t/å£ãÙr] Ë‡ØÎf‘ƒÊý{3Ôé·Ú(1v%f M*	mj÷)p80çÌ*iÚ	´NY]Qó“�úF7î&ƒÕ!Ò÷ÀnãýJ„<Ú§hc	€íÝS^Ð° ÔW=sÛRM–	�Á¦™?ZÉë-Z8H‚|Õ{×q�ö2+‘ïõÄŽâ4À´fäcl³ÜVbz»/úÌAŒJó—J¦!´wŽ¢§>²gíþœ´o)Ó9ˆûÀaÂúÔÿ�¥å�Ô š"É—�šl“ÐðÔ"ËdâÝöÝÜàÃ•p“#j×y`”�ñ^(iÞçšchãN‰œæ—øóÜÿp˜5›Ý¼iH‚Ó(Ö]ùØ×oÄ×lçµR\LÂé¿Xá·‚è¦þÉ–<÷ ûÉÎ!zœ˜ÌAÚ‰Š5µÝ`ò—}Î‘4‚4›»¦Bt:dƒº%ìÔ.RúóðÀJçbñ¬LÊ}b–¦85lÃƒ¨£F°B~aáMÅjå`¸e»e½Ë	'«EPÏ£G®oÍ`ëra“ïßdýä¸jdP¶u"1[1ŠŽõá‡ÆFÏ§ú	ÿ³ïr¸y·šÚÈjÚóÔ™àîDÒ®¿ªÖÇ3A­å«GMÔÆ“®¥óYÊ¢çÃÐßÂÍÃ{ÇZ‹¿ØOC."]Š¬
­]ÀÄ[åüec«'y·X9'›Ò2:êŸ¯˜\&„#Á)¼}~ÏŽ^4<4±jâÔ£eö¨ÅÎ¼•½£Mi�¬@9Y†uºÉºbÿöß4¢=ƒŽÍèm)¯ÓÂìŽ o`<Ÿ˜ŠàU¹Ý¸RËf^ÇpÄtË#Õ¤ÉÓ/—:ØŸÝ›[ ŠM½ª{ÖRrûÜœNtáañ£?jRM&Ÿ:=,K{£˜e­©kžŒ·7$ãF�vÿwOpdå¼³¦äRòE*ªv)Z˜gbªrÀ—¿1rÝta8ŸzAí¤ÄMóù÷c�ê.s…fà�¹×ÌÖƒHþøÓ{k�Q��zÖH@l˜¬tuèbû%5+ñÖ¿¦-Çš-"›wŸ2h@¯�YALA"`unA:¬H©Ä³^¥«¬lcÈÙ<á6¦]j
cîTÇßÍqXD»Àª´7�ù#æœÓËœFÁ²ŸÅ¾$/’ð¤fîÇ€ûþs4°#,¸!�Êc¦r¤LêQÜá±ÍÿŽ=xNîîë99Ýb "�“%Æy‡è4¬Ýg³€Kp½‚�)–ÀR”;nùÌŒÿ8¦¥
(_¦ØÒÖIYêopÄ9zRô}íû"ÚÝÔ…/ïpÝÇÓÓÉY=^ƒûÜãáHK7©@øòáR`h…3¾­Œp ½Z ¡äE`Þ¦/À��qYó¾¢Û×®{¡v ³»©Ÿ%�Žï‘Æe�·²üðdÌŽfS‹‹A6¯N(Ru*ØtÑæ@®ßÆ=ö`ªJH[dL÷uÛÎÀ‡•²Yý@þG}˜�ô'¾"‰_Á×ÐÜ3Ì€Û¾K8yK�!öÛCcýt² I2åª\¬…i¹,ð�H”ÿ–›Øéž†œ?ÄHNF	è¨h­ÜÕ�öÞ§tcÅ’æÊú GBôXš »gqðØ¼òLëóž’pWm”¤0ògþ?Ã®�3ÌPTÖqÛ¼À˜‡"ÜJ`ž
k±8üºÑneF1|y*‚¯jþs	�Å76Œc8riôí×G&CÃõ½ð…Ü%9Öx®ŠÆƒVÝökÉz‰HJuùÈm§`Ë³0ÝwŽß%‘/ç£= fê¹(ŒN{H}Ç÷üwrÖ%éÆ¿þó2Cì5£Xçó-ñ¾/BºŠÑ"Ç9_
›ÜW"WwòL.™'ÿ’:ÛI¯PÐF¸#íÞ&œWNòÀ[ñSÅyÅC³³Æ5ÝÛ>_ÜÇUO�I¤ØI9,Ùuq$Šß|*©åI‘¨ÒèX‚ÃçŠ/bÏì€——Îb šÅåÖÊÖV¾×…ÈzêÌ5nƒŸç•ØSUe.šÐ$íþ<Áš".Ø}:-tÃP·’�Ó|)¸a£´³§Q[q™võLà^ØúpŸ¸mõ#¦ÈŒš#GŠŸaVHŠ|óçÔÞLî;ZµM£)%±’{D5[ì—öjw¶ÇÆ¿Õ´H78èÔ‹¡…§ãÂfÇ6v2Nðš—éì^/ØB¶¬Ü.t¨ãìÝm½®#¨ÂëCÔ�'*�–xæ’]x«­·»hKúUÎÉ
ã¿·û \e®}íº€Üš0DG²0h�Fd>‰Tuú×ÕËƒM&4íÎP ”
ºwrƒä}Ê×kîß"qCc&Í?�à…Ä‹\DNt{¾kl@¨3¢æÂútd†³	¯êÄ#œð�K¤“¥zq`�™=‘ŸM¿Q ÷ö
.·×Q½Ge£C&/®áêºÍåÖ¦±'ŒÚŸÅ“�@†3ÖÿŠ‚¿€•RŠöº·pÞ×Õ«=lôçª z[õ|ô›çÑ/ÅåØÒep\dE±1×˜Õw?mórŒß«{Pc~åäßñÞŠ‹].ÒÃà¯K­eèt½\Óg'Åïb·ƒ/ÿbàÃ7`ßºG^ÕkÀ½gâxÊü¦ðçöîã,ÜÄ˜ÓM‡6:oý?Ã3�JØËh>vö#¥Uëy8ˆ’…ÆÀ¨‡1u_²ÑéÜ(=ŽòuIM27ŸZ0žŠöÜø^+&ó;5ÐCó[”µ‡~@þlx@9œã‘ü@@ÖÉ¿œ¿#äPTMsÿZŸ û,hÙæ‡b÷ÂZgw‚ñ>”�c¢pF¸dæÃ×€ÎšsÒ5{�¨yÃ	�Eó	íõRtÊþ `ì=4ú7öºÏ�†Yœ©²ß–j>Vj5î–ó�·¢aº‘Á�P^I-Ïj»p‚Âkm¦x'Vd,”µ¥H×— –K²Jó¸–/øj»y#Q$ç…å7IVØJýÐ¥Þó¥Îï‘w¥ã0Á|¾sbæ~}Š$—*Î¨º”z0MÔê)³‡i¯Ué”Ì8…z¨Û0@*'iAuî"&5?w$<¿\‰<*=_‹w@åýÅ+Ê�$SgîÑíØîÃ5=›úêû´©¯OÜ½®'í‘fEóú¡:bÕ;’H†×‚Ó“½[JkO5ŽEu0"Ôi{¾ÑÄQ�4Ñ“ýËÐI½óqè
V“] ø‰„yN§OÝÐ…ŠÃ-ë(˜qaœÅy' ˆØ“5€4Æ…Õ_WÝ@÷Uf¶½~fÙ[33K¨Œ¢ë,nSÂ" X×†:à3-OþuIu5^¾Îþ¥¦s¸‚Ì8‚B`~Ê—Ù*Ê=.·ÛpUˆW$Óô÷¾ó)>“Ø%�ƒ(&¿7f„9L0;þÜNÏ+1ž¾*ò�’àñ34Ê‡,¡Ø7ñ²\â´˜¬¿wi°öÛZ:Ó'…qUÝª'¬EçøvÊbX¤ƒ¨±ªGm«©�í÷¡wÄkm$�çÎm��j®ê Ðå‹6^Ú,úJNñ6ÕC8˜;Î¸„×zbæÁÿŠØÅýŒ7´bÖŽ%k»B‚[Ïî>¿è¹åš3Å¯zØ­Ó¦Õn(¹ø?šåm.’ÍŠ¨F2°Ê#	, ÄÁ›œ*«O;Ê‹/ŠM'2OdÁ4.ÿF´þ�y}e;nøm?6ÜISY=ûÈ…<R˜S‹çÕ4ÀéŒv@¡Ümqòÿ§YR-MòÀ�	1Zß5• kƒåp®t¦O'´dNÉúÍ=2#æÖ+¸£öqùÚÝ«Â¾®é¢ ÌR÷-©�!Â5áí1ºöººVa�…±cêø0�u8
ÕsÄL¬š¼kDÊ‰M
.Aîé^�€'/~ÿˆ’ïí|Û­ÐÀÔÂ]ÏÖu´6ç7ÝðÊÒ†£OQ‡„^Üž§Õ�¶ìgÆê1{ô‘†"™fõý+oW¬�¼W_7A=� ‰qdh�Äl‹ó|ï DÖ^Uý›Ñ×€9ñsàMãv”{I)Þü x¥aÕ®ËÃ‹›©ü’Â�µ£=ÖŒ²Q·Äy‘Ä#1ÈPÄVêÂ™½¤gùGÊo9[�
TÇFÈŸ¬<y¬àisÕ$óQ~Êì&×«ßdÀÀtw÷u‘¡Óü¯¨¦©”5„%lÚê4^‘ðC……<)æ)DåA¿0eBú=÷Ä»/Bž^�Š@dç½I~íw7nÙÊaªøm™¦%ƒ…žQÒO]úŸ6îô›a_¨¥+<}^²Í�ˆÚðs!ZUT2]óË�6­Eèw]J¦ŸÀÆŽëjhÜa‘ÀSŽyB’
iùÒto&‚6b¢V4ºs­ròŠoãá„«*W@¦]–QÉ�ÿ¬Sýhÿ`�µµk§ÀÎ�¢î,ÕHé8Á§¸É4vÛ»ôþÜÞ¦z×}¦Dn‚"Ý§5�àóI¿bqOA¡R0JzÖ~y yä¢uAÃuçÍãzR±±»ð¼ù4¸Î·O‡æÌyÖ“BÖŽ2÷ÉûmI9Èðàì-`³ÚYcQñoDVçˆ6Ör7‡L?õ²ä9
§Ž"\/¸ëÞ‰wœ&(…]FøZf¿°FPírÇ@†€^4fqG*nd¨JØcÓÅîøñA0P¢b?•ÀjÒ9X|èº.o :gAZ‚ç‚÷Jûme\µá“5èB ˆ~Zñ[�àÂåÜ4=[$�ÝóŽñs£ QË@ç}`[p‰Ì�Àì;âH±D“\¯Ì·ðÎ­—š°©æÓ3sË:CxZÌ‘a“Uø·_89[¯OWÏ"gÁ Æ0ü�KÂpéà)ÿAæ³(©0at#ŒðlFËïJ´û^ŽäÎ¥rî¡ÙøýY ŸÍþÝÇá'À"Ñƒböc	›8Ý,vX1—Q}x½mPÍ.Yöb×¯Ü]e« ñÉ2É¨«¦šÔô*.ƒD´ai0˜§ct ²‘ü>´¤m•am<£dÊßx$#*lLÏ¦ª%³ÐÁÞ»‡Ÿw.²�˜É°¯ª¥ZÂky½©)}ùTÑª³ñ;Ì—Pü]À¶>-çueŸ�¬j=Œ€¹«âhNë6ü�Vn,_ã|ð¼<°QI“'ÍF¶íëVÍº�-9qâ×‚)±—¼¯}–æ^òkËÆ—à˜ÅòÔÍs©Å°M�ÿÀCtîtW`b|MöÖNgøI@½èj9™�êˆñÏò©l	ßz³WmÑíÛ¸>Áj ¹Õwí1+ËóX½4€.gùÔò9#÷¨¸Ü®¨ð¶òG9@{õ*&¤3®&A+aˆ�©ž®œ�ŸÛ§�k™Gó34­ÂÄû>uÀyÚ‹eÝ›5gf_;»ø�â½vÇ<eê|?ˆIHj&Ûm5f<\µî:öhö ¨ü‹¼žèž“¯Ð2¨4‘Æ�e¦^=Òî­‘çÓ;èðh¡º·3À´É(þÙÂà#Ò×²¢Ò¼À¦—m½*‚¹†ø‘ØJküë$ñ,¬‹tòÞÞ?ÅY#guŠÂ!æŒ¨Íç)T7W^ùyÖÐ¨' ý¸Ž…œÕ·ÑòvòéOß¿fâï;à,^#“¤Ñ‹�>ñ­®'\+G�¬÷I¾qF:*txŒüë1nâù\eBow,"Í4ñwFIŽiVåÍ½§>§¦4ÏÔ oì#ñ�|èÃî	Ýï%Ÿž¤?|¡�[tžÑešjq™k‚ÿ<­m¶Pu1ñ�ÁÛäµLrO®±Õ)�xñË¡Ç©4Å¯ø’åˆ¡s¼¢.˜-�ÃfÌC`câ¾À›"gÈm�6÷Ô4(0<±aºt>°Ç~ãÞ­²¬ØÈ.5(=‰¶£*T‘‹N=Éø,0©0ƒ{ôE¥Pi"Nm¹&°<Òû ²;‘™½ˆÌt†a'éè~ûTB3ÑÜHÅ¾"¹¡±£+y™�M"@ú€¾Î—¤çvi¬0ˆØ«58ÞbŸ®¹…ÊIû¼ûn1 ŒÐz"r3ÑÚÎ‚8+ýéñóÿ¨xY§•ñÚSÄgÖÊ§tœ;w×WaŒº/:‚þ¿�hÚ¾Ãm«öC‡0ÊAÔÃ
|¨emcxÏo†{ã8äûµŽü¢YÝ[dô—¯@x<ìàƒžúÎ›Nr	Þ6ÊüŠŒP®«üé¨ÿØ÷s éüvóS¸œ-lÔlª=1îAñö:§ü;±ÖýïÂ6
¦üXŠ¢‡[Š%¬/%	…�Ëµ!™úÝæ�s ¡,s…‰h‘€äÑ·î>ÕZXû3×xÿBB“1¼5SÆF®m)8G/ÛÇŠ‹ó�¢~©ÙGÌVXau¬[´‘’²ßº	*§P9Ò‡3‚ŸC"LtB³@f²Y*9ýÀç‘b Aã¢x¢æAÎ™ç’‹‹L(kÏ2Xý,kËJÇ4.º¸³»kÔ´û—GßCÃ�~‘€â£¦ò•è8FÃ­œý™U“ô¿Ú,<Óß"Pù™vñÉTÍ:Ê�‰‚‘vg?œÇYuo¨ÏqÝß`®×ƒF[�Ä¦»�ê¸©mÃ°a”xãù-ë,ËÙR~Úi�Xó1°¾7Ë§�'îÊ'´|H3°âÖñSØµ¢ÂÖ"·O‹¾nN;¿¤ŠF†%™Àõ˜‰Ì.»¯KªÿN{¦B�Oµ33Xz`Í˜*ñ`´éÁ¸¢1ÃâAø’ÍôâÏë¸Zöd@D%�mBö.ËÌL¨EÊ9™”o®•·Üožéï°§aÅ‹ô†Ó¨l%âö:ºö";•°[ËÕ ¯§·"D£œ‰£e�)ÄP‰¨XXx^fç7ÛHUaË‡Ø �§‚ÄUTOŠƒ_ªßïdí²t¾î¡¦Isñ¾fàãµZ†¬rXï92ÒâeØ/fZýý�ÌsòÉŽo#ûO†²f´Ìd`žH¨Ê#ÒXá Y·ÿEó½t ÚˆË�ÿGN¤(!ëÑ6M½
êç^Û“®ãaŒ§)=Ë/YY:Ùž„$oJÆ¦p5ZsÅ·„ÐÔ I™q>‹K/%±ÇŒaõª-l`EßTtåõîC"½F˜ø÷Ôj½±•%.TûF°Â6Þ@ý5 ý¦­~ ÛMn;T^MRD­	 ©I¡!\ÇêçºØ	U£Ûm,P�cÌ‚W+¤µ
ñÉ¤þƒ@V²]iõ´z„}Þ8¼ ¬†€8DVr«€*Zª×&ÍN·OÝCm†žN ){•Ãß£�@ìAÏè¥Ì¨ÚËž#mA9²º¡ŠKÑãJQŸASKÁY ÞŠÐ½Tšâ$Ó¼B$‡AˆTÑ•©¸¡w»´Ï²o]Oã2¾i‡®ÇöDtÊ«¿>,‡RÎòÌ›=>~ÓëHúQþÛNíˆªh‘Ì&ý.¿Ù…#Š„�$”ðKDâ)¼£FÝ<tbWuÙ•µ¹•Þø®XðÊørÊ(Þ4&1µdôçš·Ã€¶pPÍ-{‰ã¹¤\u*eÆ¬¼µ‘Šú/fþ,.ácÖ‡ô#)ã¬Ó;Ù«Ø‰U‰È#õ!² A=Aj¹ºh9úöcœµpÛË0$3«G¿3ý@Q9N.ïøy}¡`Cºí9tšc6GZ“”-þ`IRi«S=„R¶fa#IÀ>‘¨û$,P±&‚wÚÎø±ðpÏã~ý¾‡P˜Üš‚j�¤ëQé®aH$kÿDs�Ö\Ö(„d
O/~o/÷kýÊüøJ2)ßEgÃ_y¨…G‰”µY‹Æç¼v1ÕDý“zúŸ»™—kHÿß2"8!ð&³Û3¹Âˆ˜ÖC< Ù›úŸŸêQt�Îón!VÌ8€¥Vq°€Šá¾× isÌÿº×)£±‹oÇC—äÍ8z¤0±ßs!—q&øÆ²S3‚NÊõ¸ƒ¿%ÿàžá€Þu!(åüæ¿Ãvpx¤;ìò—Lc•F¥ÿ’L]êþº3sÓa�á½•=ËÍð•„aj$ ƒËíY³"X21¸Z[J+N ?…·»Èùß{çîÆ|l]…¨ BvXó)Þ¾â§¨¯‡‚)ÙY-¿‘f¢! ;r“Nû¶ç¦îy½ƒN”Y�uRÑé¡5cw3}dœÛôã¯‡Ÿ“¿DÇG`~ä1í@¦!u†êÓwûöÆ�!Ž¦¥7OÊ^jIº4Ïê)óÖ#{DŠ�_–Ð7°‡uWQ¬òþHŸþkØ±oØT¦4})ÀSÖ´#ÔˆD™¦X ÜÁÃÐ¼<ZyþåMèí$ç °æÎ·Ç4e3Ýþ/9áÏ½M+3I„¹+B¦Ý_t¸Zq¸$f™NªãyÇ|Ê.?¢óUÑ.©‰QmŠgSCJ¼_yã¾kŽº½§Í0lh¦ïg³l¢�;ç,¥,:SL’Î¤ÞSÝÞÇši¶=äÅ).ÆêõàœS‚,XlÍG1—ŠÛ½Ú—&õ"FLIºház»´[>rGúÞµh»©�ñÓ×9¸5bÔãora¶võùn·7mx¹í@8šÔž_qù�Ttâó'à¬Í•Ù/—Hö(;sÛ†WRÌ‹âèôß*jùðœ;ÞÞ7]Ïþ=	Ãºig£¿ç<“?jñËß¯Ï˜¯ÌÕÞ!-›~p‰;vÖsßÃ€Rxßš7˜Æ*:×¹Ü ,ÃZŸqÔO"2ÐƒÕ¤n¬É$q"Óíy–«§åÊŽG/:¬Ú’/@$Øm{$°ð,cßd]NÑ45Š�Eº°ögdí2b íÆPì%KÆMõ–¦Ú¸¦ZRÈ1”¸kúL
9YÕxFEÝÁ®„h·­·Ò|i³Ëøná¬xòºÞÇ¿Cáf?§�‹ŒKÀP´Ï}ë –Œ,Z÷`WôÒS'í)è	ÌÙæ´õg«1   H?¶W“=ù¡ »4ó² Õ¦h2±Ägû    YZ   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  5ü<?xml version="1.0"?><Features nextValidID="30"><Feature recentID="0" name="AmpR" directionality="1" translationMW="31558.16" type="CDS" allowSegmentOverlaps="0" cleavageArrows="8013" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment name="signal sequence" range="7945-8013" color="#ccffcc" type="standard" translated="1"/><Segment range="8014-8805" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"/></Q></Feature><Feature recentID="1" name="EGFP" directionality="1" translationMW="26942.08" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="4102-4104" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="4105-4107" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="4108-4821" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK*"/></Q></Feature><Feature recentID="2" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="5210-5809" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"/></Q></Feature><Feature recentID="3" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5876-6464" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8976-9564" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7359-7814" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4871-5200" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2234-2537" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="RRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1366-1599" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="3' LTR (Î”U3)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6551-6784" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;self-inactivating&amp;nbsp;3' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="RSV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="294-520" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Rous sarcoma virus enhancer/promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2538-2741" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="521-701" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5051-5186" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7017-7152" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="HIV-1 Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="748-873" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of human immunodeficiency virus type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6856-6977" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="cPPT/CTS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2095-2211" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;central polypurine tract and central termination sequence of HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="AmpR promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7840-7944" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="lac promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-35" range="149-154" color="#ffffff" type="standard"/><Segment range="155-172" color="#ffffff" type="standard"/><Segment name="-10" range="173-179" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="CAP binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="113-134" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="T3 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="248-266" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T3 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="T7 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7173-7191" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="187-203" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="M13 rev" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="211-227" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="25" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7201-7217" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="26" name="Factor Xa site" directionality="2" translationMW="473.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="6346" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="5963" maxFusedRunOn="5963" detectionMode="exactProteinMatch"><Segment range="6347-6358" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="IEGR"/></Q></Feature><Feature recentID="27" name="linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4821" maxFusedRunOn="4821"><Segment range="4072-4086" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="GGGGS"/></Q></Feature><Feature recentID="28" name="linker" directionality="1" translationMW="333.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4821" maxFusedRunOn="4821"><Segment range="4087-4101" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="GGGGS"/></Q></Feature><Feature recentID="29" name="TARDBP" directionality="1" translationMW="44741.00" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4821" maxFusedRunOn="4821"><Segment range="2824-4065" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSEYIRVTEDENDEPIEIPSEDDGTVLLSTVTAQFPGACGLRYRNPVSQCMRGVRLVEGILHAPDAGWGNLVYVVNYPKDNKRKMDETDASSAVKVKRAVQKTSDLIVLGLPWKTTEQDLKEYFSTFGEVLMVQVKKDLKTGHSKGFGFVRFTEYETQVKVMSQRHMIDGRWCDCKLPNSKQSQDEPLRSRKVFVGRCTEDMTEDELREFFSQYGDVMDVFIPKPFRAFAFVTFADDQIAQSLCGEDLIIKGISVHISNAEPKHNSNRQLERSGRFGGNPGGFGNQGGFGNSRGGGAGLGNNQGSNMGGGMNFGAFSINPAMMAAAQAALQSSWGMMGMLASQQNQSGPSGNNQNQGNMQREPNQAFGSGNNSYSGSNSGAAIGWGSASNAGSGSGFNGGFGSSMDSKSSGWGM"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ¬<Notes>
<UUID>405fb08c-114c-411e-be3e-1395441a7d06</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pLV3-CMV-TARDBP(human)-EGFP-Puro</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="0:9:52">2018.9.13</Created>
<LastModified UTC="23:50:57">2026.3.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y     K         
?????                                                                                                       ÿ d      DO  d              E              ÿÿÿÿYô? P                                                                                                                                                             c<StrandColors><TopStrand><ColorRange range="0..2816" colors="black"/><ColorRange range="2817..2822" colors="blue"/><ColorRange range="4065..9738" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..2816" colors="black"/><ColorRange range="2817..2822" colors="blue"/><ColorRange range="4065..9738" colors="black"/></BottomStrand></StrandColors>   3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
